34 results on '"Long, Yang"'
Search Results
2. The role of oxidative stress in diabetes mellitus-induced vascular endothelial dysfunction
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An, Ying, Xu, Bu-tuo, Wan, Sheng-rong, Ma, Xiu-mei, Long, Yang, Xu, Yong, and Jiang, Zong-zhe
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- 2023
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3. Risk factors for postoperative recurrence in patients with stage II colorectal cancer
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Xiong, Zhi-Zhong, Xie, Ming-Hao, Li, Xian-Zhe, Jin, Long-Yang, Zhang, Feng-Xiang, Yin, Shi, Chen, Hua-Xian, and Lian, Lei
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- 2023
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4. Effects of SGLT2 inhibitors on cardiac function and health status in chronic heart failure: a systematic review and meta-analysis
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Chen, Jiao, Jiang, Chunxia, Guo, Man, Zeng, Yan, Jiang, Zongzhe, Zhang, Dongmin, Tu, Mengqin, Tan, Xiaozhen, Yan, Pijun, Xu, XunMei, Long, Yang, and Xu, Yong
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- 2024
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5. Correction to: KDM6B promotes ESCC cell proliferation and metastasis by facilitating C/EBPβ transcription
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Qin, Mei, Han, Fei, Wu, Jian, Gao, Feng-xia, Li, Yuan, Yan, De-xin, He, Xue-mei, Long, Yang, Tang, Xiao-ping, Ren, De-lian, Gao, Yan, and Dai, Tian-yang
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- 2021
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6. KDM6B promotes ESCC cell proliferation and metastasis by facilitating C/EBPβ transcription
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Qin, Mei, Han, Fei, Wu, Jian, Gao, Feng-xia, Li, Yuan, Yan, De-xin, He, Xue-mei, Long, Yang, Tang, Xiao-ping, Ren, De-lian, Gao, Yan, and Dai, Tian-yang
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- 2021
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7. Resistant starch ameliorated insulin resistant in patients of type 2 diabetes with obesity: a systematic review and meta-analysis
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Gao, Chenlin, Rao, Mingyue, Huang, Wei, Wan, Qin, Yan, Pijun, Long, Yang, Guo, Man, Xu, Youhua, and Xu, Yong
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- 2019
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8. Circular RNA FOXP1 promotes tumor progression and Warburg effect in gallbladder cancer by regulating PKLR expression
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Wang, Shouhua, Zhang, Yongjie, Cai, Qiang, Ma, Mingzhe, Jin, Long Yang, Weng, Mingzhe, Zhou, Di, Tang, Zhaohui, Wang, Jian Dong, and Quan, Zhiwei
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- 2019
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9. IL-6 and IL-8 secreted by tumour cells impair the function of NK cells via the STAT3 pathway in oesophageal squamous cell carcinoma
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Wu, Jian, Gao, Feng-xia, Wang, Chao, Qin, Mei, Han, Fei, Xu, Tao, Hu, Zhi, Long, Yang, He, Xue-mei, Deng, Xin, Ren, De-lian, and Dai, Tian-yang
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- 2019
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10. A 5G-powered robot-assisted teleultrasound diagnostic system in an intensive care unit
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Shaobo Duan, Luwen Liu, Shuaiyang Wang, Liuwei Hao, Yongqing Chen, Ye Zhang, Lianzhong Zhang, and Long Yang
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Adult ,Male ,medicine.medical_specialty ,Intestinal gas ,020205 medical informatics ,Image quality ,Pleural effusion ,Vital signs ,02 engineering and technology ,Critical Care and Intensive Care Medicine ,Diagnostic system ,030218 nuclear medicine & medical imaging ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Robotic Surgical Procedures ,law ,0202 electrical engineering, electronic engineering, information engineering ,medicine ,Humans ,Bedside ultrasound ,Critical care medicine ,Medical diagnosis ,Telemedical ,Aged ,Ultrasonography ,Aged, 80 and over ,Internet ,business.industry ,Research ,lcsh:Medical emergencies. Critical care. Intensive care. First aid ,Remote critical care ultrasound ,lcsh:RC86-88.9 ,Middle Aged ,Robot-assisted ,medicine.disease ,Intensive care unit ,Telemedicine ,Intensive Care Units ,Teleultrasound ,Emergency medicine ,Remote critical care medicine ,Female ,business ,5G - Abstract
Background Teleultrasound provides an effective solution to problems that arise from limited medical resources, a lack of local expertise, and scenarios where the risk of infection is high. This study aims to explore the feasibility of the application of a 5G-powered robot-assisted teleultrasound diagnostic system in an intensive care unit. Methods In this study, the robot-assisted teleultrasound diagnostic system MGIUS-R3 was used. Using 5G network technology, the doctor manipulates the robotic arm to perform teleultrasound examination. The doctor can adjust parameters via the teleultrasound control panel, and real-time transmission of audio, video and ultrasound images can facilitate simultaneous communication between both parties. All patients underwent robot-assisted teleultrasound examination and bedside ultrasound examination of the liver, gallbladder, pancreas, spleen, kidney, as well as assessment for pleural effusion and abdominal effusion. We evaluated the feasibility of the application of the robot-assisted teleultrasound diagnosis system in the intensive care unit in terms of consultation duration, image quality, and safety. We also compared diagnostic consistency and differences. Results Apart from one patient who was excluded due to severe intestinal gas interference and poor image quality, a total of 32 patients were included in this study. Every patient completed all relevant examinations. Among them, 20 patients were male; 12 were female. The average age of the patients was 61 ± 20 years. The average duration of teleultrasound diagnosis was 17 ± 7 min. Of the 32 patients, 26 had positive results, 6 had negative results, and 5 had inconsistent diagnoses. The overall diagnostic results were basically the same, and there were no differences in diagnostic levels between the two. The overall average image quality score was 4.73 points, which represented a high-quality image. After robot-assisted teleultrasound examination, no significant changes were observed in the vital signs of patients as compared to before examination, and no examination-related complications were found. Conclusion The 5G-powered robot-assisted teleultrasound diagnostic system was associated with the benefits of clear images, simple operation, relatively high levels of consistency in terms of diagnostic results, higher levels of safety, and has considerable application value in the intensive care unit.
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- 2021
11. KDM6B promotes ESCC cell proliferation and metastasis by facilitating C/EBPβ transcription.
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Mei Qin, Fei Han, Jian Wu, Feng-xia Gao, Yuan Li, De-xin Yan, Xue-mei He, Yang Long, Xiao-ping Tang, De-lian Ren, Yan Gao, Tian-yang Dai, Qin, Mei, Han, Fei, Wu, Jian, Gao, Feng-Xia, Li, Yuan, Yan, De-Xin, He, Xue-Mei, and Long, Yang
- Abstract
Background: As an H3K27me3 demethylase and counteracts polycomb-mediated transcription repression, KDM6B has been implicated in the development and malignant progression in various types of cancers. However, its potential roles in esophageal squamous cell carcinoma (ESCC) have not been explored.Methods: The expression of KDM6B in human ESCC tissues and cell lines was examined using RT-qPCR, immunohistochemical staining and immunoblotting. The effects of KDM6B on the proliferation and metastasis of ESCC were examined using in vitro and in vivo functional tests. RNA-seq and ChIP-seq assay were used to demonstrate the molecular biological mechanism of KDM6B in ESCC.Results: We show that the expression level of KDM6B increased significantly in patients with lymph node metastasis. Furthermore, we confirmed that KDM6B knockdown reduces proliferation and metastasis of ESCC cells, while KDM6B overexpression has the opposite effects. Mechanistically, KDM6B regulates TNFA_SIGNALING_VIA_NFκB signalling pathways, and H3K27me3 binds to the promoter region of C/EBPβ, leading to the promotion of C/EBPβ transcription. Besides, we show that GSK-J4, a chemical inhibitor of KDM6B, markedly inhibits proliferation and metastasis of ESCC cells.Conclusions: The present study demonstrated that KDM6B promotes ESCC progression by increasing the transcriptional activity of C/EBPβ depending on its H3K27 demethylase activity. [ABSTRACT FROM AUTHOR]- Published
- 2021
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12. Comparative genome-wide characterization leading to simple sequence repeat marker development for Nicotiana
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Qingshi Zhao, Xuewen Wang, Long Yang, Yulong Gao, Shuai Yang, Jeffrey L. Bennetzen, Shumeng Zhang, Meng Li, and Yongdui Chen
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0301 basic medicine ,Genetic Markers ,lcsh:QH426-470 ,Genotype ,lcsh:Biotechnology ,Computational biology ,Genotyping technology ,Genome ,03 medical and health sciences ,Tandem repeat ,lcsh:TP248.13-248.65 ,Tobacco ,Databases, Genetic ,Genetics ,Genotyping ,Nicotiana ,Molecular breeding ,Marker polymorphism ,Comparative Genomic Hybridization ,Polymorphism, Genetic ,biology ,fungi ,food and beverages ,Amplicon ,biology.organism_classification ,SSR ,Marker database ,lcsh:Genetics ,030104 developmental biology ,Genetic marker ,Microsatellite ,Genome, Plant ,Software ,Biotechnology ,Research Article ,Internet Access ,Microsatellite Repeats - Abstract
Background Simple sequence repeats (SSRs) are tandem repeats of DNA that have been used to develop robust genetic markers. These molecular markers are powerful tools for basic and applied studies such as molecular breeding. In the model plants in Nicotiana genus e.g. N. benthamiana, a comprehensive assessment of SSR content has become possible now because several Nicotiana genomes have been sequenced. We conducted a genome-wide SSR characterization and marker development across seven Nicotiana genomes. Results Here, we initially characterized 2,483,032 SSRs (repeat units of 1–10 bp) from seven genomic sequences of Nicotiana and developed SSR markers using the GMATA® software package. Of investigated repeat units, mono-, di- and tri-nucleotide SSRs account for 98% of all SSRs in Nicotiana. More complex SSR motifs, although rare, are highly variable between Nicotiana genomes. A total of 1,224,048 non-redundant Nicotiana (NIX) markers were developed, of which 99.98% are novel. An efficient and uniform genotyping protocol for NIX markers was developed and validated. We created a web-based database of NIX marker information including amplicon sizes of alleles in each genome for downloading and online analysis. Conclusions The present work constitutes the first deep characterization of SSRs in seven genomes of Nicotiana, and the development of NIX markers for these SSRs. Our online marker database and an efficient genotyping protocol facilitate the application of these markers. The NIX markers greatly expand Nicotiana marker resources, thus providing a useful tool for future research and breeding. We demonstrate a novel protocol for SSR marker development and utilization at the whole genome scale that can be applied to any lineage of organisms. The Tobacco Markers & Primers Database (TMPD) is available at http://biodb.sdau.edu.cn/tmpd/index.html Electronic supplementary material The online version of this article (10.1186/s12864-018-4878-4) contains supplementary material, which is available to authorized users.
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- 2018
13. The modified pancreatic stent system for prevention of post-ERCP pancreatitis: a case-control study.
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Cheng Zhang, Yu-long Yang, Yue-feng Ma, Hong-wei Zhang, Jing-yi Li, Mei-ju Lin, Li-jun Shi, Chun-chun Qi, Zhang, Cheng, Yang, Yu-Long, Ma, Yue-Feng, Zhang, Hong-Wei, Li, Jing-Yi, Lin, Mei-Ju, Shi, Li-Jun, and Qi, Chun-Chun
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PANCREATITIS , *SURGICAL stents , *ENDOSCOPIC retrograde cholangiopancreatography , *TREATMENT effectiveness , *SURGICAL complications , *LIPASES , *PROSTHETICS , *TIME , *PILOT projects , *RETROSPECTIVE studies , *CASE-control method , *ACUTE diseases , *MEDICAL device removal , *PREVENTION - Abstract
Background: Prophylactic pancreatic stents after endoscopic retrograde cholangiopancreatography (ERCP) can help prevent post-ERCP pancreatitis. However most of the pancreatic stents need to be removed by another ERCP. The aim of this observational study was to investigate the feasibility and effectiveness of the modified pancreatic stent system for prevention of post-ERCP pancreatitis.Methods: From November 2013 to November 2015, a total of 230 patients who had prophylactic pancreatic stent placed for prevention of post-ERCP pancreatitis at a single institution were identified and stratified. In this case-control design, 150 patients received an ordinary pancreatic stent, and 80 patients received the modified pancreatic stent. The main outcome measures were the difficulty level and complications of pancreatic stent placement and extraction between the two groups.Results: In ordinary group, the average time of pancreatic stent and nasal biliary drainage placement was 3.5 ± 0.6 min. There were 13 cases of stent proximal migration (8.7%), 20 cases of stent spontaneous abscission (13.3%), 5 cases of acute pancreatitis (3.3%) (2 cases for stent abscission) and 7 cases of hyperamylasemia (4.7%) after ERCP. One hundred thirty patients received extra duodenoscope (86.7%) to remove the stent, and 4 cases had acute pancreatitis and 5 patients had hyperamylasemia after removing the proximal migratory stents. In modified group, the average time of pancreatic stent system placement was 4.9 ± 0.7 min, but there was only one case of stent abscission (1.3%), 2 cases of acute pancreatitis (2.5%) and 3 cases of hyperamylasemia (3.8%). The new pancreatic stents were removed directly under x-ray without complication.Conclusions: The modified pancreatic stent system has the same effect of preventing post-ERCP pancreatitis, lower rate of stents proximal migration and spontaneous abscission, and the advantage of easier removed compared with ordinary pancreatic stent. [ABSTRACT FROM AUTHOR]- Published
- 2017
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14. Chinese version of the Constant-Murley questionnaire for shoulder pain and disability: a reliability and validation study.
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Min Yao, Long Yang, Zuo-yuan Cao, Shao-dan Cheng, Shuang-lin Tian, Yue-li Sun, Jing Wang, Bao-ping Xu, Xiao-chun Hu, Yong-jun Wang, Ying Zhang, Xue-jun Cui, Yao, Min, Yang, Long, Cao, Zuo-Yuan, Cheng, Shao-Dan, Tian, Shuang-Lin, Sun, Yue-Li, Wang, Jing, and Xu, Bao-Ping
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SHOULDER pain , *QUESTIONNAIRES , *MUSCULOSKELETAL system , *CHINESE language , *DISABILITIES , *PUBLIC health , *COMPARATIVE studies , *FUNCTIONAL assessment , *FACTOR analysis , *HEALTH surveys , *RESEARCH methodology , *MEDICAL cooperation , *HEALTH outcome assessment , *QUALITY of life , *RESEARCH , *SHOULDER , *TRANSLATIONS , *EVALUATION research , *PAIN measurement , *PSYCHOLOGY ,RESEARCH evaluation - Abstract
Background: Shoulder pain is a common musculoskeletal disorder in Chinese population, which affects more than 1,3 billion individuals. To the best of our knowledge, there has been no available Chinese-language version of measurements of shoulder pain and disability so far. Moreover, the Constant-Murley score (CMS) questionnaire is a universally recognized patient-reported questionnaire for clinical practice and research. The present study was designed to evaluate a Chinese translational version of CMS and subsequently assess its reliability and validity.Methods: The Chinese translational version of CMS was formulated by means of forward-backward translation. Meanwhile, a final review was carried out by an expert committee, followed by conducting a test of the pre-final version. Therefore, the reliability and validity of the Chinese translational version of CMS could be assessed using the internal consistency, construct validity, factor analysis, reliability and floor and ceiling effects. Specifically, the reliability was assessed by testing the internal consistency (Cronbach's α) and test-retest reliability (intraclass coefficient correlation [ICC]), while the construct validity was evaluated via comparison between the Chinese translational version of CMS with visual analog scale (VAS) score and the 36-Item Short Form Health Survey (SF-36, Spearman correlation).Results: The questionnaire was verified to be acceptable after distribution among 120 subjects with unilateral shoulder pain. Factor analysis had revealed a two-factor and 10-item solution. Moreover, the assessment results indicated that the Chinese translational version of CMS questionnaire harbored good internal consistency (Cronbach's α = 0.739) and test-retest reliability (ICC = 0.827). In addition, the Chinese translational version of CMS was moderately correlated with VAS score (r = 0.497) and SF-36 (r = 0.135). No obvious floor and ceiling effects were observed in the Chinese translational version of CMS questionnaire.Conclusion: Chinese translational version of CMS exhibited good reliability, which is relatively acceptable and is likely to be widely used in this population. [ABSTRACT FROM AUTHOR]- Published
- 2017
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15. Modified eggshell procedure via posterior approach for sclerosing thoracic disc herniation: a preliminary study.
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Si-Dong Yang, Qian Chen, Sheng-Hua Ning, Wen-Yuan Ding, and Da-Long Yang
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MENINGES ,ANALYSIS of variance ,COMPUTED tomography ,INTERNAL fixation in fractures ,INTERVERTEBRAL disk displacement ,NEUROLOGIC manifestations of general diseases ,ORTHOPEDIC surgery ,PROBABILITY theory ,RESEARCH funding ,SPINAL fusion ,SUBDURAL hematoma ,SURGICAL complications ,THORACIC vertebrae ,WOUNDS & injuries ,SURGICAL equipment ,PILOT projects ,TREATMENT effectiveness ,PRE-tests & post-tests ,REPEATED measures design ,RETROSPECTIVE studies ,DATA analysis software ,MUSCLE weakness ,DESCRIPTIVE statistics ,MANN Whitney U Test ,SURGERY - Abstract
Background: Clinically, sclerosing thoracic disc herniation is a disease with high surgical risk and various complications. Eggshell procedure is a surgical method used by surgeons to treat sclerosing thoracic disc herniation. The aim of this study was to report a modified eggshell procedure to treat sclerosing thoracic disc herniation. Methods: Medical records of 25 patients with sclerosing thoracic disc herniation were collected between 2007/01 and 2010/08, including 14 males and 11 females, with an average age of 51.7 years old. Modified eggshell procedure was performed to treat the patients with sclerosing thoracic disc herniation. All patients were followed up. Japanese Orthopaedic Association (JOA) score was used to evaluate the clinical outcomes. Results: All operations were performed successfully with complication rate of 12 %. There were 2 cases of dural laceration and 1 subdural hematoma. All included patients were followed up for at least 5 years, with the median of 6 years. JOA score of preoperation was 5 (IQR = 1) while it was 8 (IQR = 2) at final follow-up, with significant difference (Mann-Whitney U test, Z= -4.891, P<0.001).The improvement rate of neurological status was 51.5 ±23. 1 %. According to the classification of improvement rate, there were 15 cases at good level, 8 cases at moderate level, and 2 cases without any improvement. Conclusions: Modified eggshell procedure is a safe and effective surgical method when performed to treat sclerosing thoracic disc herniation in the clinical practice. [ABSTRACT FROM AUTHOR]
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- 2016
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16. Effects of social support, hope and resilience on quality of life among Chinese bladder cancer patients: a cross-sectional study.
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Meng-Yao Li, Yi-Long Yang, Li Liu, Lie Wang, Li, Meng-Yao, Yang, Yi-Long, Liu, Li, and Wang, Lie
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BLADDER cancer treatment , *QUALITY of life , *REGRESSION analysis , *SOCIAL support , *CANCER risk factors , *MENTAL health , *HOPE , *PSYCHOLOGICAL resilience , *ASIANS , *CROSS-sectional method , *PSYCHOLOGY ,BLADDER tumors - Abstract
Background: Improvement of quality of life has been one of goals in health care for people living with bladder cancer. Meanwhile, positive psycho-social variables in oncology field have increasingly received attention. However, the assessment of quality of life of bladder cancer patients and the integrative effects of positive psycho-social variables has limited reporting. The aim of this study was to assess quality of life as well as the integrative effects of social support, hope and resilience on quality of life among Chinese bladder cancer patients.Methods: A cross-sectional study was conducted at the First Hospital of China Medical University in Liaoning Province, China. A total of 365 bladder cancer patients eligible for this study completed questionnaires on demographic variables, FACT-BL, Perceived Social Support Scale, Adult Hope Scale, and Resilience Scale-14 during July 2013 to July 2014.Results: The average score of FACT-BL was 87.60 ± 16.27 (Mean ± SD). Hierarchical regression analyses indicated that social support, hope and resilience as a whole accounted for 30.3 % variance of quality of life. Under standardized estimate (β) sequence, social support, hope and resilience significantly and positively associated with quality of life, respectively.Conclusions: Quality of life for bladder cancer patients was at a low level in China, which should receive more attention in Chinese medical institutions. More importantly, efforts to increase social support, hope and resilience might be useful to support the quality of life among Chinese bladder cancer patients. [ABSTRACT FROM AUTHOR]- Published
- 2016
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17. Choledochoscopic high-frequency needle-knife electrotomy for treatment of anastomotic strictures after Roux-en-Y hepaticojejunostomy.
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Yu-long Yang, Cheng Zhang, Ping Wu, Yue-feng Ma, Jing-yi Li, Hong-wei Zhang, Li-jun Shi, Mei-ju Lin, Ying Yu, Yang, Yu-Long, Zhang, Cheng, Wu, Ping, Ma, Yue-Feng, Li, Jing-Yi, Zhang, Hong-Wei, Shi, Li-Jun, Lin, Mei-Ju, and Yu, Ying
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JEJUNOSTOMY , *SURGICAL complications , *SURGICAL anastomosis , *SURGICAL stents ,BILIARY tract surgery - Abstract
Background: Anastomotic stricture is a complex and substantial complication following Roux-en-Y hepaticojejunostomy. Initially, endoscopic and percutaneous approaches are often attempted, but the gold standard remains surgical biliary reconstruction, especially for refractory stricture. However, this solution leaves much room for improvement, due to the challenging nature of the biliary reconstruction procedure, in which anastomotic stricture may still occur.Aims: To investigate the feasibility and effectiveness of choledochoscopic high-frequency needle-knife electrotomy as an intervention in the treatment of anastomotic strictures following Roux-en-Y hepaticojejunostomy.Methods: From February 2010 to October 2014, clinical data was collected and retrospectively compared for patients who underwent balloon dilation or/and choledochoscopic high-frequency needle-knife electrotomy for the treatment of anastomotic strictures after Roux-en-Y hepaticojejunostomy.Results: A total of 38 patients underwent successful choledochoscopic treatment and all the anastomotic strictures were removed successfully, 19 of which were treated with electrotomy, 7 with balloon dilation, and 12 with both electrotomy and balloon dilation. Among these groups,the average operating times were 6.9 ± 2.4 min,10.1 ± 6.8 min, and 20.2 ± 13.5 min, respectively. The average stent supporting times were 6.3 ± 0.7 months, 6.5 ± 0.6 months, and 6.1 ± 0.4 respectively. The mean follow-up after stent removal was 42.1 ± 27.4 months, and in 26.3 % (5/19), 28.5 % (2/7) and 16.7 % (2/12) of cases, recurrent anastomotic stricture occurred. Of these 9 total patients with recurrent anastomotic, two patients were successfully rescued by full-covered self-expanding removable metal stents and 7 patients by electrotomy combined with balloon dilation.Conclusions: Choledochoscopic high-frequency needle-knife electrotomy is both feasible and safe in the treatment of anastomotic stricture after Roux-en-Y hepaticojejunostomy, with a similar long-term outcome to balloon dilation in treating anastomotic stricture after Roux-en-Y hepaticojejunostomy. A combination of choledochoscopic electrotomy concurrent with balloon dilation should be recommended based on the low rate of recurrence. [ABSTRACT FROM AUTHOR]- Published
- 2016
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18. Identification of genomic sites for CRISPR/ Cas9-based genome editing in the Vitis vinifera genome.
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Yi Wang, Xianju Liu, Chong Ren, Gan-Yuan Zhong, Long Yang, Shaohua Li, and Zhenchang Liang
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GENOME editing ,HUMAN genome ,PLANT genomes ,MICROBIAL genes ,ANIMALS - Abstract
Background: CRISPR/Cas9 has been recently demonstrated as an effective and popular genome editing tool for modifying genomes of humans, animals, microorganisms, and plants. Success of such genome editing is highly dependent on the availability of suitable target sites in the genomes to be edited. Many specific target sites for CRISPR/Cas9 have been computationally identified for several annual model and crop species, but such sites have not been reported for perennial, woody fruit species. In this study, we identified and characterized five types of CRISPR/Cas9 target sites in the widely cultivated grape species Vitis vinifera and developed a user-friendly database for editing grape genomes in the future. Results: A total of 35,767,960 potential CRISPR/Cas9 target sites were identified from grape genomes in this study. Among them, 22,597,817 target sites were mapped to specific genomic locations and 7,269,788 were found to be highly specific. Protospacers and PAMs were found to distribute uniformly and abundantly in the grape genomes. They were present in all the structural elements of genes with the coding region having the highest abundance. Five PAM types, TGG, AGG, GGG, CGG and NGG, were observed. With the exception of the NGG type, they were abundantly present in the grape genomes. Synteny analysis of similar genes revealed that the synteny of protospacers matched the synteny of homologous genes. A user-friendly database containing protospacers and detailed information of the sites was developed and is available for public use at the Grape-CRISPR website (http://biodb.sdau.edu.cn/gc/index.html). Conclusion: Grape genomes harbour millions of potential CRISPR/Cas9 target sites. These sites are widely distributed among and within chromosomes with predominant abundance in the coding regions of genes. We developed a publicly-accessible Grape-CRISPR database for facilitating the use of the CRISPR/Cas9 system as a genome editing tool for functional studies and molecular breeding of grapes. Among other functions, the database allows users to identify and select multi-protospacers for editing similar sequences in grape genomes simultaneously. [ABSTRACT FROM AUTHOR]
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- 2016
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19. Traumatic neuroma in a patient with breast cancer after mastectomy: a case report and review of the literature
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Xiao qu Hu, Quan Li, Hong ye Hu, Er li Gao, and Yin long Yang
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Adult ,medicine.medical_specialty ,medicine.medical_treatment ,Breast surgery ,lcsh:Surgery ,Case Report ,Breast Neoplasms ,Traumatic neuroma ,lcsh:RC254-282 ,Neuroma ,Breast cancer ,Postoperative Complications ,Biopsy ,medicine ,Carcinoma ,otorhinolaryngologic diseases ,Humans ,Neoplasms, Post-Traumatic ,Mastectomy ,Recurrent Breast Carcinoma ,medicine.diagnostic_test ,business.industry ,Carcinoma, Ductal, Breast ,lcsh:RD1-811 ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Prognosis ,Surgery ,Review Literature as Topic ,Oncology ,Female ,business - Abstract
The incidence of traumatic neuroma is extremely low, especially in those patients with breast cancer after mastectomy. There are only 10 cases reported in the literature. We report a patient who developed a palpable nodular mass near the mastectomy scar. The result of excisional biopsy was traumatic neuroma. Review of the literature reveal 10 cases with breast cancer of traumatic neuromas after mastectomy. Traumatic neuroma is a benign lesion and a reparative response of the nerve to injury, either direct/indirect trauma or chronic inflammation. Benign lesions as traumatic neuromas are more rarely seen after mastectomy. However, in order to manage patients' treatment, the most critical problem is to distinguish it from recurrent breast carcinoma. Although assistant examination methods such as ultrasound and computed tomography are valuable to a certain extent, the final diagnosis can only be confirmed on pathologic examination.
- Published
- 2012
20. Population pharmacokinetic modeling of the Qishe pill in three major traditional Chinese medicine-defined constitutional types of healthy Chinese subjects: study protocol for a randomized controlled trial.
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Yue-li Sun, Ting Hou, Shu-fen Liu, Zhong-liang Zhang, Ning Zhang, Min Yao, Long Yang, Qi Shi, Xue-jun Cui, and Yong-jun Wang
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PHARMACOKINETICS ,DRUG metabolism ,HERBAL medicine ,PATHOLOGY ,METABOLOMICS - Abstract
Background: High incidences of neck pain morbidity are challenging in various situations for populations based on their demographic, physiological and pathological characteristics. Chinese proprietary herbal medicines, as Complementary and Alternative Medicine (CAM) products, are usually developed from well-established and long-standing recipes formulated as tablets or capsules. However, good quantification and strict standardization are still needed for implementation of individualized therapies. The Qishe pill was developed and has been used clinically since 2009. The Qishe pill's personalized medicine should be documented and administered to various patients according to the ancient TCM system, a classification of personalized constitution types, established to determine predisposition and prognosis to diseases as well as therapy and life-style administration. Therefore, we describe the population pharmacokinetic profile of the Qishe pill and compare its metabolic rate in the three major constitution types (Qi-Deficiency, Yin-Deficiency and Blood-Stasis) to address major challenges to individualized standardized TCM. Methods/Design: Healthy subjects (N = 108) selected based on constitutional types will be assessed, and standardized pharmacokinetic protocol will be used for assessing demographic, physiological, and pathological data. Laboratory biomarkers will be evaluated and blood samples collected for pharmacokinetics(PK) analysis and second-generation gene sequencing. In single-dose administrations, subjects in each constitutional type cohort (N = 36) will be randomly divided into three groups to receive different Qishe pill doses (3.75, 7.5 and 15 grams). Multiomics, including next generation sequencing, metabolomics, and proteomics, will complement the Qishe pill's multilevel assessment, with cytochrome P450 genes as targets. In a comparison with the general population, a systematic population pharmacokinetic (PopPK) model for the Qishe pill will be established and verified. [ABSTRACT FROM AUTHOR]
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- 2015
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21. Genome-wide distribution comparative and composition analysis of the SSRs in Poaceae.
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Yi Wang, Chao Yang, Qiaojun Jin, Dongjie Zhou, Shuangshuang Wang, Yuanjie Yu, and Long Yang
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GRASS genetics ,PLANT gene mapping ,COMPARATIVE genomics ,GRASS breeding ,GENETIC markers in plants - Abstract
Background: The Poaceae family is of great importance to human beings since it comprises the cereal grasses which are the main sources for human food and animal feed. With the rapid growth of genomic data from Poaceae members, comparative genomics becomes a convinent method to study genetics of diffierent species. The SSRs (Simple Sequence Repeats) are widely used markers in the studies of Poaceae for their high abundance and stability. Results: In this study, using the genomic sequences of 9 Poaceae species, we detected 11,993,943 SSR loci and developed 6,799,910 SSR primer pairs. The results show that SSRs are distributed on all the genomic elements in grass. Hexamer is the most frequent motif and AT/TA is the most frequent motif in dimer. The abundance of the SSRs has a positive linear relationship with the recombination rate. SSR sequences in the coding regions involve a higher GC content in the Poaceae than that in the other species. SSRs of 70-80 bp in length showed the highest AT/GC base ratio among all of these loci. The result shows the highest polymorphism rate belongs to the SSRs ranged from 30 bp to 40 bp. Using all the SSR primers of Japonica, nineteen universal primers were selected and located on the genome of the grass family. The information of SSR loci, the SSR primers and the tools of mining and analyzing SSR are provided in the PSSRD (Poaceae SSR Database, http://biodb.sdau.edu.cn/pssrd/). Conclusions: Our study and the PSSRD database provide a foundation for the comparative study in the Poaceae and it will accelerate the study on markers application, gene mapping and molecular breeding. [ABSTRACT FROM AUTHOR]
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- 2015
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22. The effects of psychological interventions on depression and anxiety among Chinese adults with cancer: a meta-analysis of randomized controlled studies.
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Yi-Long Yang, Guo-Yuan Sui, Guang-Cong Liu, De-Sheng Huang, Si-Meng Wang, and Lie Wang
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CANCER patients , *MENTAL depression , *ANXIETY disorders , *DISEASE complications , *CHINESE people , *DISEASE prevalence , *MEDICAL databases , *RANDOMIZED controlled trials , *DISEASES - Abstract
Background Our previous studies found the high prevalence of depression and anxiety among Chinese cancer patients, and many empirical studies have been conducted to evaluate the effects of psychological interventions on depression and anxiety among Chinese cancer patients. This study aimed to conduct a meta-analysis in order to assess the effects of psychological interventions on depression and anxiety in Chinese adults with cancer. Methods The four most comprehensive Chinese academic database- CNKI, Wanfang, Vip and CBM databases-were searched from their inception until January 2014. PubMed and Web of Science (SCIE) were also searched from their inception until January 2014 without language restrictions, and an internet search was used. Randomized controlled studies assessing the effects of psychological interventions on depression and anxiety among Chinese adults with cancer were analyzed. Study selection and appraisal were conducted independently by three authors. The pooled random-effects estimates of standardized mean difference (SMD) and 95% confidence intervals (CI) were calculated. Moderator analysis (meta-regression and subgroup analysis) was used to explore reasons for heterogeneity. Results We retrieved 147 studies (covering 14,039 patients) that reported 253 experimental-control comparisons. The random effects model showed a significant large effect size for depression (SMD = 1.199, p < 0.001; 95% CI = 1.095-1.303) and anxiety (SMD = 1.298, p < 0.001; 95% CI = 1.187-1.408). Cumulative meta-analysis indicated that sufficient evidence had accumulated since 2000-2001 to confirm the statistically significant effectiveness of psychological interventions on depression and anxiety in Chinese cancer patients. Moderating effects were found for caner type, patients' selection, intervention format and questionnaires used. In studies that included lung cancer, preselected patients with clear signs of depression/anxiety, adopted individual intervention and used State-Trait Anxiety Inventory (STAI), the effect sizes were larger. Conclusions We concluded that psychological interventions in Chinese cancer patients have large effects on depression and anxiety. The findings support that an adequate system should be set up to provide routine psychological interventions for cancer patients in Chinese medical settings. However, because of some clear limitations (heterogeneity and publication bias), these results should be interpreted with caution. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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23. The association between occupational stress and depressive symptoms and the mediating role of psychological capital among Chinese university teachers: a cross-sectional study.
- Author
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Xue Shen, Yi-Long Yang, Yang Wang, Li Liu, Shu Wang, and Lie Wang
- Abstract
Background: Depression is a major public health problem that affects both individuals and society. Previous studies report that university teachers are particularly susceptible to high levels of occupational stress and depressive symptoms. The aims of this study were to explore the association between occupational stress and depressive symptoms in a group of university teachers, and assess the mediating role of psychological capital between these variables. Methods: A cross-sectional study was performed between November 2013 and January 2014. Teachers from six universities were randomly sampled in Shenyang. The Center for Epidemiologic Studies Depression Scale, effort-reward imbalance scale, and psychological capital questionnaire (PCQ-24), as well as questions about demographic and working factors, were administered in questionnaires distributed to 1,500 university teachers. Completed questionnaires were received from 1,210 participants. Hierarchical linear regression analysis was used to examine the mediating role of psychological capital. Results: In the present study, 58.9% (95% CI (Confidence Intervals): 56.1% to 61.7%) of university teachers had a CES-D score equal to or above the cut-off of 16. Both effort–reward ratio (ERR) and scores of over-commitment were positively associated with depressive symptoms, whereas psychological capital was negatively associated with depressive symptoms among university teachers. Psychological capital partially mediated the relationship between occupational stress and depressive symptoms. Conclusions: Among Chinese university teachers, occupational stress may be a risk factor for depressive symptoms, whereas psychological capital might be protective against depressive symptoms. Our results suggest that college administrators could support the development of psychological capital in their staff to alleviate depressive symptoms. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
24. Resampling methods to reduce the selection bias in genetic effect estimation in genome-wide scans
- Author
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Sophia S. F. Lee, Lei Sun, Shelley B. Bull, Haijiang Steven Shi, and Long Yang Wu
- Subjects
Shrinkage estimator ,lcsh:QH426-470 ,media_common.quotation_subject ,Locus (genetics) ,Biology ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,Resampling ,Genetics ,Test statistic ,False positive paradox ,Humans ,Genetics(clinical) ,Genetics (clinical) ,Selection Bias ,030304 developmental biology ,media_common ,Selection bias ,0303 health sciences ,030305 genetics & heredity ,Estimator ,Chromosome Mapping ,lcsh:Genetics ,Proceedings ,Microsatellite ,Genome-Wide Association Study ,Microsatellite Repeats - Abstract
Using the simulated data of Problem 2 for Genetic Analysis Workshop 14 (GAW14), we investigated the ability of three bootstrap-based resampling estimators (a shrinkage, an out-of-sample, and a weighted estimator) to reduce the selection bias for genetic effect estimation in genome-wide linkage scans. For the given marker density in the preliminary genome scans (7 cM for microsatellite and 3 cM for SNP), we found that the two sets of markers produce comparable results in terms of power to detect linkage, localization accuracy, and magnitude of test statistic at the peak location. At the locations detected in the scan, application of the three bootstrap-based estimators substantially reduced the upward selection bias in genetic effect estimation for both true and false positives. The relative effectiveness of the estimators depended on the true genetic effect size and the inherent power to detect it. The shrinkage estimator is recommended when the power to detect the disease locus is low. Otherwise, the weighted estimator is recommended.
- Published
- 2005
25. Cold hardiness and biochemical response to low temperature of the unfed bush tick Haemaphysalis longicornis (Acari: Ixodidae).
- Author
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Zhi-Jun Yu, Yu-Lan Lu, Xiao-Long Yang, Jie Chen, Hui Wang, Duo Wang, and Jing-Ze Liu
- Abstract
Background: The survival of overwintering ticks, is critical for their subsequent population dynamics in the spring, and consequent transmission of tick-borne diseases. Survival is largely influenced by the severity of the winter temperatures and their degree of cold hardiness at the overwintering stage. The bush tick Haemaphysalis longicornis, is widely distributed in China, and can transmit various pathogens that pose serious medical/veterinary problems. In the present study we investigated the effect of low temperature stress to tick survival, super-cooling point and body content of water, glycerol and total protein. Methods: After various temperature acclimations, the super-cooling point was measured by Ni/CrNi-thermocouples with a precision temperature recorder. Water content was determined from weight loss of the sample exposed to 60°C for 48 h. Glycerol content was determined using Free Glycerol Reagent as directed by the manufacturer, and total protein was determined using the Bradford assay. Results: The 50% mortality temperatures for the adults and nymphs were −13.7°C and −15.2°C, respectively; and the discriminating temperatures for the adults and nymphs were −16.0°C and −17.0°C, respectively. The super-cooling points of the adults and nymphs were −19.0°C and −22.7°C, respectively. The water content of adult H. longicornis decreased substantially after acclimation at 0°C for 10 d, whereas the nymphs decreased after acclimation at 0°C for 20 d, and the glycerol and proteins of both nymphs and adults were significantly increased (p < 0.01) when stressed at 0°C for 10 d. Conclusions: In H. longicornis, low temperature stress can enhance its cold hardiness and trigge [ABSTRACT FROM AUTHOR]
- Published
- 2014
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26. The prevalence of depression and anxiety among Chinese adults with cancer: a systematic review and meta-analysis.
- Author
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Yi-Long Yang, Li Liu, Yang Wang, Hui Wu, Xiao-Shi Yang, Jia-Na Wang, and Lie Wang
- Subjects
- *
ANXIETY , *CHINESE people , *CANCER patient psychology , *META-analysis , *DISEASES - Abstract
Background: A lot of empirical studies have been conducted to evaluate the prevalence of depression and anxiety among Chinese adults with cancer. We aimed to conduct a meta-analysis in order to evaluate the prevalence and odds ratios of depression and anxiety in Chinese adults with cancer compared with those without. Methods: The three most comprehensive computerized Chinese academic databases-CNKI, Wangfang and Vip databases-were systematically screened through September 2012. PubMed and Web of Science (SCIE) were also searched from their inception until September 2012 without language restrictions, and an internet search was also used. Case-control studies assessing the prevalence of depression and anxiety among Chinese adults with cancer were analyzed. Study selection and appraisal were conducted independently by three authors. The non-weighted prevalence, pooled random-effects estimates of odds ratio (OR) and 95% confidence intervals (CI) were all calculated. Results: Seventeen eligible studies with a total of 3497 subjects were included. The prevalence of depression and anxiety were significantly higher in adults with cancer compared with those without (Depression: 54.90% vs. 17.50%, OR = 7.85, 95% CI = 5.56-11.07, P = 0.000; Anxiety: 49.69% vs. 18.37%, OR = 6.46, 95% CI = 4.36-9.55, P = 0.000), the same situation was also observed in subgroup of control groups, assessment methods and cancer types. Although no difference of depression was observed in studies utilizing clinical diagnosis compared with self-report, the OR of anxiety in adults with cancer compared with those without was higher in studies utilizing clinical diagnosis (OR = 8.42, 95% CI = 4.83-14.70) than self-reports (OR = 5.83, 95% CI = 3.64-9.34). The ORs of depression and anxiety in cancer patients compared with disease group (Depression: OR = 6.03, 95% CI = 4.23-8.61; Anxiety: OR = 4.40, 95% CI = 3.05-6.36) were lower than in those compared with normal group (Depression: OR = 13.58, 95% CI = 6.26-29.46; Anxiety: OR = 15.47, 95% CI = 10.00-23.95). Conclusions: We identified high prevalence rates of depression and anxiety among Chinese adults with cancer. The findings support that the prevalence of depression and anxiety among adults with cancer should receive more attention in Chinese medical settings. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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- View/download PDF
27. Nuclear β-catenin accumulation is associated with increased expression of Nanog protein and predicts poor prognosis of non-small cell lung cancer.
- Author
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Xi-Qing Li, Xing-Long Yang, Gong Zhang, Si-Pei Wu, Xu-Bing Deng, Sheng-Jun Xiao, Qiu-Zhen Liu, Kai-Tai Yao, and Guang-Hui Xiao
- Subjects
- *
CATENINS , *PROGNOSIS , *SMALL cell lung cancer , *PROTEINS , *IMMUNOHISTOCHEMISTRY , *CYTOPLASM , *IMMUNOFLUORESCENCE , *EPIDERMAL growth factor receptors - Abstract
Background: Although the prognostic roles of β-catenin expression in non-small cell lung cancer (NSCLC) have been reported in several immunohistochemical (IHC) studies, the results were not consistent because some studies lack sufficient number of the positive cases or did not evaluate the subcellular localization features of the protein. Method: In this study, we have evaluated the expression levels and subcellular localization of β-catenin and Nanog proteins IHC staining in tissue specimens from 309 patients with NSCLC, and explored their association with clinicopathological features and patient outcome. Results: We showed that patients with negative expression of membranous beta-catenin had a trend towards shorter survival (p=0.064) than those with positive expression. In contrast to previous studies, we found that increased expression of either cytoplasmic or nuclear β-catenin was strongly associated with poor prognosis and was an independent prognosticator for overall survival (p <0.01). We further found that NSCLC cells frequently exhibited an abundance of nuclear Nanog protein which was significantly correlated with nuclear β-catenin expression (p <0.01) and poor prognosis (p <0.01). Interestingly, immunofluorescent staining results revealed that increased expression of Nanog and nuclear translocation of β-catenin occurred concomitantly in response to epidermal growth factor receptor (EGFR) signaling in A549 and H23 cells. Furthermore, western blot analysis show that nuclear β-catenin rather than cytoplasmic β-catenin expression in the A549 and H23 cells can be enhanced by adding EGF, Nanog expression in the A549 and H23 cells with knockdown of β-catenin can not be obviously enhanced by adding EGF. Conclusion: We propose that evaluation of subcellular localization of β-catenin and Nanog expression is of clinical significance for patients with NSCLC. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
28. ECM-related gene expression profile in vascular smooth muscle cells from human saphenous vein and internal thoracic artery.
- Author
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Tian-xiang Zhu, Bin Lan, Ling-ying Meng, Yan-long Yang, Rui-xiong Li, En-min Li, Shao-yi Zheng, and Li-yan Xu
- Subjects
VASCULAR smooth muscle ,CORONARY restenosis ,GENE expression ,EXTRACELLULAR matrix ,SAPHENOUS vein ,INTERNAL thoracic artery - Abstract
Currently, Saphenous vein (SV) and internal thoracic artery (ITA) are still the most common graft materials in Coronary Artery Bypass Grafting (CABG) whereas SV graft have a lower long-term patency than ITA. Vascular smooth muscle cells (VSMCs) phenotype conversion, proliferation and migration may play a key role in mechanism of vein graft restenosis. To explore differential gene expression profile in VSMCs from SV and ITA will help to further elucidate the mechanism of VSMCs in vein graft restenosis after CABG and to provide new thread of gene therapy. Methods: VSMCs from paired SV and ITA were cultured for experiments of Affymetrix microarrays and verification using FQ RT-PCR, while the database for annotation, visualization and integrated discovery bioinformatics resources (DAVID 2.0) was utilized for bioinformatics analysis of differential gene expression profile between SV VSMCs and ITA VSMCs. RNA of tunica media from SV and ITA segments were extracted for FQ RT-PCR to display differential expression of PLAT Results: 54,613 probe sets were examined by gene microarray experiments. In SV VSMCs, 1,075 genes were up-regulated and 406 of them were higher than two-fold; 1,399 genes were down-regulated and 424 of them were lower than two-fold as compare with ITA VSMCs.14 ECM-related genes differentially expressed were verificated and listed as following: COL4A4, COL11A1, FN1, TNC, THBS, FBLN, MMP3, MMP9, TIMP3, WNT5A, SGCD were higher whereas COL14A1, ELN, PLAT lower in SV VSMCs than ITA VSMCs. In addition, PLAT was lower in tunica media from SV segments than ITA. Conclusion: VSMCs from SV and ITA have distinct phenotypes characteristics. Both promoting and inhibiting migration ECM-related genes were higher in VSMCs from SV as compared with ITA, suggesting that VSMCs from SV have more potential migrating capability whereas less PLAT both in SV VSMCs and vascular tissue,implying that SV may prone to be restenosis after CABG. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
29. Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions.
- Author
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Jin-Long Yang, Su-Hui Zhang, Zuo-Hua Liu, Rui Yang, Yong Huang, and Ming Wen
- Subjects
- *
CYTOMEGALOVIRUS diseases , *GENES , *POLYMERASE chain reaction , *TISSUES , *DNA - Abstract
Background: Porcine cytomegalovirus (PCMV) induces silent infection in adult pigs but more frequently causes fatal, generalized infection in newborn piglets. This study aimed to develop a new loop-mediated isothermal amplification (LAMP) method for the sensitive, rapid, and inexpensive detection of PCMV under field conditions. Methods: Tissue obtained from nine-week-old PCMV-free Landrace pigs or pig samples from postmortem examinations were analyzed. The samples were found to have clinical signs and lesions consistent with inclusion body rhinitis. Six specific primers were designed by targeting the PCMV DNA polymerase (DPOL) DNA. The LAMP reaction was optimized in a water bath. The sensitivity and specificity of LAMP and polymerase chain reaction (PCR) were compared. Results: PCMV DNA was amplified at 65°C, and the result could be detected as early as 30 min into the reaction. Positive reactions could be visualized by the naked eye as a color change brought on by the addition of SYBR Green. The sensitivity and specificity of LAMP were found to be similar to those of the PCR. Conclusions: LAMP is a high-throughput technique for the detection of PCMV and has a high specificity, sensitivity and simplicity; these factors make it suitable for detection of PCMV under field conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
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30. Expression of Estrogen Receptor-α and Ki67 in Relation to Pathological and Molecular Features in Early-Onset Infiltrating Ductal Carcinoma.
- Author
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Shian-ling Ding, Lai-Fa Sheu, Jyh-Cherng Yu, Tseng-Long Yang, BeFong Chen, Fur-jiang Leu, and Chen-Yang Shen
- Subjects
ESTROGEN receptors ,BREAST cancer ,IMMUNOHISTOCHEMISTRY ,CARCINOGENESIS ,POLYMORPHISM (Crystallography) - Abstract
Estrogen causes breast cancer by triggering proliferation via an estrogen receptor (ER)-mediated mechanism. However, paradoxically, ERα, one of the two known ER subtypes, and the proliferation marker, Ki67, are not usually expressed in the same breast tumor. To explore whether ERα-positive tumors and proliferating (Ki67-positive) tumors have different tumorigenic characteristics, we performed an immunohistochemical study on 74 early-onset infiltrating ductal carcinomas of the breast. To test this hypothesis, we examined whether ERα-positive and Ki67-positive tumors showed differences in (i) pathological grade, (ii) three indices of tumor grade (tubule formation, nuclear pleomorphism, and mitotic number), and (iii) expression of important proteins implicated in breast tumorigenesis (cyclin D1, ErbB2, ATM, BRCA1, Rb, p53, and p21). The results of the multigenic analysis showed that ERα and Ki67 were the only two important markers significantly and independently associated with tumor grade, consistent with the above hypothesis. ERα- positive, Ki67-negative tumors frequently displayed a low tumor grade (i.e. being well differentiated), whereas Ki67-positive, ERα-negative tumors were more likely to exhibit a high tumor grade. In addition, positive ERα expression (46 of 74 cases, 62%) correlated well with positive cyclin D1 expression (p < 0.005), less nuclear pleomorphism (p < 0.001), and a low mitotic count (p < 0.005), whereas positive Ki67 expression (36 of 74 cases, 49%) correlated with reduced BRCA1 expression (p < 0.01) and high mitotic activity (p < 0.01). These findings suggest that the expressions of ERα and Ki67 might be involved in distinct pathological and molecular features during breast cancer development. Copyright © 2004 National Science Council, ROC and S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
31. "Best practices in risk reducing bilateral salpingo-oophorectomy: the influence of surgical specialty".
- Author
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Malacarne DR, Boyd LR, Long Y, and Blank SV
- Subjects
- Adult, Female, Guideline Adherence statistics & numerical data, Gynecology standards, Humans, Middle Aged, Practice Guidelines as Topic, Prognosis, Retrospective Studies, Salpingo-oophorectomy standards, Salpingo-oophorectomy statistics & numerical data, Surgical Oncology standards, Gynecology methods, Ovarian Neoplasms prevention & control, Salpingo-oophorectomy methods, Specialization, Surgical Oncology methods
- Abstract
Background: Risk-reducing bilateral salpingo-oophorectomy (RRBSO) increases survival in patients at high risk of developing ovarian cancer. While many general gynecologists perform this procedure, some argue it should be performed exclusively by specialists. In this retrospective observational study, we identified how often optimal techniques were used and whether surgeons' training impacted implementation., Methods: We used the ACOG guidelines highlighting various aspects of the procedure to determine which elements were consistent with best practices to maximize surgical prophylaxis. All cases of RRBSO from 2006 to 2010 were identified. We abstracted data from the operative and pathology reports to review the techniques employed. Fisher's exact test and chi-square were utilized to compare differences between groups (InStat, La Jolla, CA)., Results: Among 263 RRBSOs, 22 were performed by general gynecologists and 241 by gynecologic oncologists. Gynecologic oncologists were more likely to perform pelvic washings-217/241 vs. 10/22 (p < .0001). They were more likely to include a description of the upper abdomen-220/241 vs. 12/22 (p < .0001). Oncologists were more likely to utilize a retroperitoneal approach to skeletonize the infundibulopelvic ligaments-157/241 vs. 3/22 (p < .0001). When operations were performed by oncologists, the specimens were more often completely sectioned-217/241 vs. 16/22 (p = .003). The use of a retroperitoneal approach among gynecologic oncologists increased over the study period (chi-square for trend, p < .0001). There was no visible trend in performance improvement in any other area when looking at either group., Conclusion: Gynecologic oncologists are more likely to adhere to best practice techniques when performing RRBSO, though there was room for improvement for both groups.
- Published
- 2017
- Full Text
- View/download PDF
32. Prevalence of overweight and obesity among Chinese Yi nationality: a cross-sectional study.
- Author
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Gao Y, Ran XW, Xie XH, Lu HL, Chen T, Ren Y, Long Y, and Tian HM
- Subjects
- Adult, Aged, China epidemiology, Cross-Sectional Studies, Female, Humans, Male, Middle Aged, Obesity ethnology, Overweight ethnology, Prevalence, Young Adult, Obesity epidemiology, Overweight epidemiology
- Abstract
Background: Overweight and obesity are considered a serious health problem. There are little data on the prevalence of overweight and obesity among the Yi ethnic group in China. This study aimed to investigate the epidemiologic features of overweight/obesity among Chinese Yi nationality., Methods: A cross-sectional study, including 1255 subjects aged 20-75 years, was carried out in Liangshan Yi Autonomous Prefecture of Sichuan province from 2007 to 2008. Overweight/overall obesity was defined by World Health Organization (WHO) or the Working Group on Obesity in China., Results: Overall, the prevalence of overweight and obesity was 19.0% and 2.9%, respectively, based on the WHO definition, while it was 21.0% and 7.4%, respectively, according to the Working Group on Obesity in China, which is similar to data reported in the 2002 Chinese National Nutrition and Health Survey. Urban residents had a significantly higher prevalence of obesity (WHO criteria: 4.3% vs 1.7% p = 0.008; China criteria: 11.4% vs 3.7%, p < 0.001) and overweight (WHO criteria: 28.9% vs 8.9% p < 0.001; China criteria: 31.2% vs 10.4%, p < 0.001) than that in rural residents. Older age, a family history of obesity, higher income, drinking and urban residence were significantly associated with an increased risk of overweight/obesity., Conclusions: The prevalence of overweight/obesity in the Yi nationality is similar to that in Chinese adults 5 years ago. However, urban residents have a much higher prevalence of overweight/obesity than their rural counterparts. Lifestyle and diet patterns associated with socioeconomic status may explain the difference between urban and rural residents. The prevention of overweight/obesity among urban inhabitants deserves more attention in national health education programs.
- Published
- 2011
- Full Text
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33. Comparison of family-based association tests in chromosome regions selected by linkage-based confidence intervals.
- Author
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Lewinger JP, Lee SS, Biernacka J, Wu LY, Shi HS, and Bull SB
- Subjects
- Confidence Intervals, Disease genetics, Humans, Microsatellite Repeats genetics, Physical Chromosome Mapping, Polymorphism, Single Nucleotide genetics, Reproducibility of Results, Chromosomes, Human genetics, Family, Genetic Linkage, Genome-Wide Association Study
- Abstract
We use the Genetic Analysis Workshop 14 simulated data to explore the effectiveness of a two-stage strategy for mapping complex disease loci consisting of an initial genome scan with confidence interval construction for gene location, followed by fine mapping with family-based tests of association on a dense set of single-nucleotide polymorphisms. We considered four types of intervals: the 1-LOD interval, a basic percentile bootstrap confidence interval based on the position of the maximum Zlr score, and asymptotic and bootstrap confidence intervals based on a generalized estimating equations method. For fine mapping we considered two family-based tests of association: a test based on a likelihood ratio statistic and a transmission-disequilibrium-type test implemented in the software FBAT. In two of the simulation replicates, we found that the bootstrap confidence intervals based on the peak Zlr and the 1-LOD support interval always contained the true disease loci and that the likelihood ratio test provided further strong confirmatory evidence of the presence of disease loci in these regions.
- Published
- 2005
- Full Text
- View/download PDF
34. Resampling methods to reduce the selection bias in genetic effect estimation in genome-wide scans.
- Author
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Wu LY, Lee SS, Shi HS, Sun L, and Bull SB
- Subjects
- Chromosome Mapping, Humans, Microsatellite Repeats genetics, Polymorphism, Single Nucleotide genetics, Selection Bias, Genome-Wide Association Study methods, Genome-Wide Association Study statistics & numerical data
- Abstract
Using the simulated data of Problem 2 for Genetic Analysis Workshop 14 (GAW14), we investigated the ability of three bootstrap-based resampling estimators (a shrinkage, an out-of-sample, and a weighted estimator) to reduce the selection bias for genetic effect estimation in genome-wide linkage scans. For the given marker density in the preliminary genome scans (7 cM for microsatellite and 3 cM for SNP), we found that the two sets of markers produce comparable results in terms of power to detect linkage, localization accuracy, and magnitude of test statistic at the peak location. At the locations detected in the scan, application of the three bootstrap-based estimators substantially reduced the upward selection bias in genetic effect estimation for both true and false positives. The relative effectiveness of the estimators depended on the true genetic effect size and the inherent power to detect it. The shrinkage estimator is recommended when the power to detect the disease locus is low. Otherwise, the weighted estimator is recommended.
- Published
- 2005
- Full Text
- View/download PDF
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