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Your search keyword '"Möller-Krull Maren"' showing total 2 results
Start Over Author "Möller-Krull Maren" Publisher biomed central
2 results on '"Möller-Krull Maren"'

1. Establishment and characterization of a newhuman pancreatic adenocarcinoma cell line withhigh metastatic potential to the lung.

Author

Kalinina, Tatyana, Güngör, Cenap, Thieltges, Sabrina, Möller-Krull, Maren, Penas, Eva Maria Murga, Wicklein, Daniel, Streichert, Thomas, Schumacher, Udo, Kalinin, Viacheslav, Simon, Ronald, Otto, Benjamin, Dierlamm, Judith, Schwarzenbach, Heidi, Effenberger, Katharina E., Bockhorn, Maximilian, Izbicki, Jakob R., and Yekebas, Emre F.

Subjects
PANCREATIC cancer, PROGNOSIS, CELL lines, CHROMOSOME abnormalities, RNA
Abstract

Background: Pancreatic cancer is still associated with devastating prognosis. Real progress in treatment options has still not been achieved. Therefore new models are urgently needed to investigate this deadly disease. As a part of this process we have established and characterized a new human pancreatic cancer cell line. Methods: The newly established pancreatic cancer cell line PaCa 5061 was characterized for its morphology, growth rate, chromosomal analysis and mutational analysis of the K-ras, EGFR and p53 genes. Gene-amplification and RNA expression profiles were obtained using an Affymetrix microarray, and overexpression was validated by IHC analysis. Tumorigenicity and spontaneous metastasis formation of PaCa 5061 cells were analyzed in pfp-/-/rag2-/- mice. Sensitivity towards chemotherapy was analysed by MTT assay. Results: PaCa 5061 cells grew as an adhering monolayer with a doubling time ranging from 30 to 48 hours. M-FISH analyses showed a hypertriploid complex karyotype with multiple numerical and unbalanced structural aberrations. Numerous genes were overexpressed, some of which have previously been implicated in pancreatic adenocarcinoma (GATA6, IGFBP3, IGFBP6), while others were detected for the first time (MEMO1, RIOK3). Specifically highly overexpressed genes (fold change > 10) were identified as EGFR, MUC4, CEACAM1, CEACAM5 and CEACAM6. Subcutaneous transplantation of PaCa 5061 into pfp-/-/rag2-/- mice resulted in formation of primary tumors and spontaneous lung metastasis. Conclusion: The established PaCa 5061 cell line and its injection into pfp-/-/rag2-/- mice can be used as a new model for studying various aspects of the biology of human pancreatic cancer and potential treatment approaches for the disease. [ABSTRACT FROM AUTHOR]

Published
2010
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2. Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung.

Author

Kalinina T, Güngör C, Thieltges S, Möller-Krull M, Penas EM, Wicklein D, Streichert T, Schumacher U, Kalinin V, Simon R, Otto B, Dierlamm J, Schwarzenbach H, Effenberger KE, Bockhorn M, Izbicki JR, and Yekebas EF

Subjects
Adenocarcinoma drug therapy, Adenocarcinoma genetics, Adenocarcinoma metabolism, Animals, Antineoplastic Agents pharmacology, Cell Culture Techniques, Cell Line, Tumor, Cell Proliferation, Cell Separation, Cell Shape, DNA-Binding Proteins deficiency, DNA-Binding Proteins genetics, Dose-Response Relationship, Drug, Female, Gene Expression Profiling methods, Gene Expression Regulation, Neoplastic, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Karyotyping, Lung Neoplasms drug therapy, Lung Neoplasms genetics, Lung Neoplasms pathology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Middle Aged, Mutation, Oligonucleotide Array Sequence Analysis, Pancreatic Neoplasms drug therapy, Pancreatic Neoplasms genetics, Pancreatic Neoplasms metabolism, Pore Forming Cytotoxic Proteins deficiency, Pore Forming Cytotoxic Proteins genetics, Time Factors, Tumor Burden, Xenograft Model Antitumor Assays, Adenocarcinoma secondary, Lung Neoplasms secondary, Pancreatic Neoplasms pathology
Abstract

Background: Pancreatic cancer is still associated with devastating prognosis. Real progress in treatment options has still not been achieved. Therefore new models are urgently needed to investigate this deadly disease. As a part of this process we have established and characterized a new human pancreatic cancer cell line., Methods: The newly established pancreatic cancer cell line PaCa 5061 was characterized for its morphology, growth rate, chromosomal analysis and mutational analysis of the K-ras, EGFR and p53 genes. Gene-amplification and RNA expression profiles were obtained using an Affymetrix microarray, and overexpression was validated by IHC analysis. Tumorigenicity and spontaneous metastasis formation of PaCa 5061 cells were analyzed in pfp-/-/rag2-/- mice. Sensitivity towards chemotherapy was analysed by MTT assay., Results: PaCa 5061 cells grew as an adhering monolayer with a doubling time ranging from 30 to 48 hours. M-FISH analyses showed a hypertriploid complex karyotype with multiple numerical and unbalanced structural aberrations. Numerous genes were overexpressed, some of which have previously been implicated in pancreatic adenocarcinoma (GATA6, IGFBP3, IGFBP6), while others were detected for the first time (MEMO1, RIOK3). Specifically highly overexpressed genes (fold change > 10) were identified as EGFR, MUC4, CEACAM1, CEACAM5 and CEACAM6. Subcutaneous transplantation of PaCa 5061 into pfp-/-/rag2-/- mice resulted in formation of primary tumors and spontaneous lung metastasis., Conclusion: The established PaCa 5061 cell line and its injection into pfp-/-/rag2-/- mice can be used as a new model for studying various aspects of the biology of human pancreatic cancer and potential treatment approaches for the disease.

Published
2010
Full Text
View/download PDF

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