16 results on '"Na, Yang"'
Search Results
2. Thymosin β4 reverses phenotypic polarization of glial cells and cognitive impairment via negative regulation of NF-κB signaling axis in APP/PS1 mice
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Yanbing Ma, Zi-Long Li, Shengfeng Ji, Ke-Wei Chang, Pengbo Yang, Meng Wang, Li-Rong Feng, Kai-Ge Ma, Yi-Hua Qian, John Bosco Ruganzua, Hua Han, Xinlin Chen, and Wei-Na Yang
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Genetically modified mouse ,Male ,Phenotypic polarization of glial cells and neuroinflammation ,Immunology ,Mice, Transgenic ,Biology ,Immunofluorescence ,Cellular and Molecular Neuroscience ,symbols.namesake ,Amyloid beta-Protein Precursor ,Mice ,Downregulation and upregulation ,Alzheimer Disease ,Memory ,mental disorders ,medicine ,Presenilin-1 ,Animals ,Cognitive Dysfunction ,RC346-429 ,NF-κB signaling pathway ,Neurons ,Microglia ,medicine.diagnostic_test ,General Neuroscience ,Research ,NF-kappa B ,Brain ,Cell biology ,Thymosin ,Disease Models, Animal ,medicine.anatomical_structure ,Phenotype ,Neurology ,nervous system ,Thymosin β4 ,Astrocytes ,Cognition and emotion ,Nissl body ,symbols ,TLR4 ,Immunohistochemistry ,Female ,Neurology. Diseases of the nervous system ,Alzheimer’s disease ,Neuroglia ,Astrocyte ,Signal Transduction - Abstract
Background Thymosin β4 (Tβ4) is the most abundant member of the β-thymosins and plays an important role in the control of actin polymerization in eukaryotic cells. While its effects in multiple organs and diseases are being widely investigated, the safety profile has been established in animals and humans, currently, little is known about its influence on Alzheimer’s disease (AD) and the possible mechanisms. Thus, we aimed to evaluate the effects and mechanisms of Tβ4 on glial polarization and cognitive performance in APP/PS1 transgenic mice. Methods Behavior tests were conducted to assess the learning and memory, anxiety and depression in APP/PS1 mice. Thioflavin S staining, Nissl staining, immunohistochemistry/immunofluorescence, ELISA, qRT-PCR, and immunoblotting were performed to explore Aβ accumulation, phenotypic polarization of glial cells, neuronal loss and function, and TLR4/NF-κB axis in APP/PS1 mice. Results We demonstrated that Tβ4 protein level elevated in all APP/PS1 mice. Over-expression of Tβ4 alone alleviated AD-like phenotypes of APP/PS1 mice, showed less brain Aβ accumulation and more Insulin-degrading enzyme (IDE), reversed phenotypic polarization of microglia and astrocyte to a healthy state, improved neuronal function and cognitive behavior performance, and accidentally displayed antidepressant-like effect. Besides, Tβ4 could downregulate both TLR4/MyD88/NF-κB p65 and p52-dependent inflammatory pathways in the APP/PS1 mice. While combination drug of TLR4 antagonist TAK242 or NF-κB p65 inhibitor PDTC exerted no further effects. Conclusions These results suggest that Tβ4 may exert its function by regulating both classical and non-canonical NF-κB signaling and is restoring its function as a potential therapeutic target against AD.
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- 2021
3. Long-term effects of mixed planting on arbuscular mycorrhizal fungal communities in the roots and soils of Juglans mandshurica plantations
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Yan Zhang, Lixue Yang, Na Yang, Depeng Zhang, Li Ji, and Yuchun Yang
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0106 biological sciences ,Microbiology (medical) ,Larix gmelinii ,China ,Conservation of Natural Resources ,Microbial Consortia ,lcsh:QR1-502 ,Arbuscular mycorrhizal fungi ,Juglans ,Larix ,010603 evolutionary biology ,01 natural sciences ,Microbiology ,lcsh:Microbiology ,Mixed plantation ,03 medical and health sciences ,Soil ,Juglans mandshurica ,Mycology ,Mycorrhizae ,Botany ,Acaulospora ,Humans ,DNA, Fungal ,Mycological Typing Techniques ,Glomus ,Soil Microbiology ,030304 developmental biology ,0303 health sciences ,Rhizosphere ,biology ,fungi ,Fungi ,Illumina MiSeq sequencing ,High-Throughput Nucleotide Sequencing ,Interspecific competition ,biology.organism_classification ,Soil fertility ,Research Article - Abstract
Background Establishing mixed plantations is an effective way to improve soil fertility and increase forest productivity. Arbuscular mycorrhizal (AM) fungi are obligate symbiotic fungi that can promote mineral nutrient absorption and regulate intraspecific and interspecific competition in plants. However, the effects of mixed plantations on the community structure and abundance of AM fungi are still unclear. Illumina MiSeq sequencing was used to investigate the AM fungal community in the roots and soils of pure and mixed plantations (Juglans mandshurica × Larix gmelinii). The objective of this study is to compare the differential responses of the root and rhizosphere soil AM fungal communities of Juglans mandshurica to long-term mixed plantation management. Results Glomus and Paraglomus were the dominant genera in the root samples, accounting for more than 80% of the sequences. Compared with that in the pure plantation, the relative abundance of Glomus was higher in the mixed plantation. Glomus, Diversispora and Paraglomus accounted for more than 85% of the sequences in the soil samples. The relative abundances of Diversispora and an unidentified genus of Glomeromycetes were higher and lower in the pure plantation, respectively. The Root_P samples (the roots in the pure plantation) had the highest number of unique OTUs (operational taxonomic units), which belonged mainly to an unidentified genus of Glomeromycetes, Paraglomus, Glomus and Acaulospora. The number of unique OTUs detected in the soil was lower than that in the roots. In both the root and soil samples, the forest type did not have a significant effect on AM fungal diversity, but the Sobs value and the Shannon, Chao1 and Ace indices of AM fungi in the roots were significantly higher than those in the soil. Conclusions Mixed forest management had little effect on the AM fungal community of Juglans mandshurica roots and significantly changed the community composition of the soil AM fungi, but not the diversity.
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- 2020
4. Admission fasting plasma glucose is associated with in-hospital outcomes in patients with acute coronary syndrome and diabetes: findings from the improving Care for Cardiovascular Disease in China - Acute Coronary Syndrome (CCC-ACS) project
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Nan Ye, Lijiao Yang, Guoqin Wang, Weijing Bian, Fengbo Xu, Changsheng Ma, Dong Zhao, Jing Liu, Yongchen Hao, Jun Liu, Na Yang, Hong Cheng, and on behalf of the CCC-ACS
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Cardiovascular disease in China ,lcsh:Diseases of the circulatory (Cardiovascular) system ,medicine.medical_specialty ,Acute coronary syndrome ,endocrine system diseases ,Anemia ,030209 endocrinology & metabolism ,030204 cardiovascular system & hematology ,Stress hyperglycemia ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Diabetes mellitus ,medicine ,Adverse effect ,business.industry ,Diabetes ,Fasting plasma glucose ,nutritional and metabolic diseases ,Glycosylated hemoglobin ,medicine.disease ,Cardiac surgery ,lcsh:RC666-701 ,Heart failure ,Cardiology and Cardiovascular Medicine ,business ,Research Article - Abstract
Background The discrepancy between glycosylated hemoglobin (HbA1c) and fasting plasma glucose (FPG) in clinical practice may be related to factors such as acute stress, renal dysfunction, and anemia, and its relationship with in-hospital outcomes is uncertain. The aim of this study was to investigate the association between the type of discrepancy between HbA1c and FPG and in-hospital outcomes in patients with acute coronary syndrome (ACS) and diabetes. Methods The Improving Care for Cardiovascular Disease in China - Acute Coronary Syndrome (CCC-ACS) project is a national, hospital-based quality improvement project with an ongoing database. Patients with ACS, diabetes and complete HbA1c and FPG values at admission were included. The consistent group included patients with HbA1c 1c ≥ 6.5% and FPG ≥ 7.0 mmol/L. The discrepancy group included patients with HbA1c ≥ 6.5% and FPG 1c group) or HbA1c Results A total of 7762 patients were included in this study. The numbers of patients in the consistent and discrepancy groups were 5490 and 2272 respectively. In the discrepancy group, increased HbA1c accounted for 77.5% of discrepancies, and increased FPG accounted for 22.5% of discrepancies. After adjusting for confounders, patients in the increased FPG group had a 1.6-fold increased risk of heart failure (OR, 1.62; 95% CI, 1.08–2.44), a 1.6-fold increased risk of composite cardiovascular death and heart failure (OR, 1.63; 95% CI, 1.09–2.43), and a 1.6-fold increased risk of composite major adverse cardiovascular and cerebrovascular events (MACCEs) and heart failure (OR, 1.56; 95% CI, 1.08–2.24) compared to patients in the increased HbA1c group. Conclusions Patients with an increased FPG but normal HbA1c had a higher risk of in-hospital adverse outcomes than those with increased HbA1c but normal FPG. This result may indicate that when HbA1c and FPG are inconsistent in patients with ACS and diabetes, the increased FPG that may be caused by stress hyperglycemia may have a more substantial adverse effect than increased HbA1c, which may be caused by chronic hyperglycemia. These high-risk patients should be given more attention and closer monitoring in clinical practice. Trial registry Clinicaltrial.gov, NCT02306616. Registered 29 November 2014.
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- 2020
5. Identification and characterization of DNA endonucleases in Plasmodium falciparum 3D7 clone
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Qijun Chen, Zhiwei Tu, Ying Feng, Yiwei Zhang, Na Yang, Xiaoyu Sang, Ning Jiang, and Jianping Li
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0301 basic medicine ,lcsh:Arctic medicine. Tropical medicine ,Erythrocytes ,lcsh:RC955-962 ,DNA repair ,Plasmodium falciparum ,Schizonts ,DNA endonuclease ,Protozoan Proteins ,Catalysis ,lcsh:Infectious and parasitic diseases ,law.invention ,03 medical and health sciences ,Endonuclease ,chemistry.chemical_compound ,0302 clinical medicine ,Plasmid ,law ,Deoxyribonuclease I ,lcsh:RC109-216 ,Gene ,biology ,Chemistry ,Merozoites ,Research ,Sequence Analysis, DNA ,biology.organism_classification ,Recombinant Proteins ,Malaria ,030104 developmental biology ,Infectious Diseases ,Biochemistry ,biology.protein ,Recombinant DNA ,Parasitology ,030217 neurology & neurosurgery ,DNA - Abstract
Background Plasmodium falciparum is the most virulent parasite of the five Plasmodium species that cause human malaria, and biological analysis of the parasite is critical for the development of novel strategies for disease control. DNA endonucleases are important for maintaining the biological activity, gene stability of the parasite and interaction with host immune systems. In this study, ten sequences of DNA endonucleases were found in the genome of P. falciparum 3D7 clone, seven of them were predicted to contain an endonuclease/exonuclease/phosphatase (IPR005135) domain which plays an important role in DNA catalytic activity. The seven DNA endonucleases of P. falciparum were systematically investigated. Methods Plasmodium falciparum 3D7 clone was cultured in human O+ RBCs, RNA was extracted at 8, 16, 24, 32, 40, and 48 h post invasion and real-time quantitative PCR was carried out to analyse the transcription of the seven DNA endonuclease genes in asexual stages. Immunofluorescence assay was carried out to confirm the location of the encoded proteins expressed in the erythrocytic stages. Finally, the catalytic activity of the DNA nucleases were tested. Results Of the seven proteins analysed, two proteins were not soluble. Fragments derived from the rest five endonuclease sequences were successfully expressed as soluble proteins, and which were used to generate antisera for protein localization. The proteins were all located in the nucleus at ring and trophozoite stages. While at schizont stage, proteins encoded by PF3D7_1238600, PF3D7_0107200 and PF3D7_0319200 were in the punctuated forms in the parasite most likely around nuclei of the merozoites. But the proteins encoded by PF3D7_0305600 and PF3D7_1363500 were distributed around the infected erythrocyte membrane. The enzymatic activity of the recombinant GST-PF3D7_1238600 was very efficient without divalent iron, while the activity of the rest four enzymes was iron dependent. Further, divalent irons did not show any specific enhancement on the activity of GST-PF3D7_1238600, but the activity of GST-PF3D7_0107200, GST-PF3D7_1363500 and GST-PF3D7_0319200 were Cu2+ dependent. The activity of GST-PF3D7_0305600 was dependent on Mg2+ and Mn2+. Except GST-PF3D7_1363500, four of the GST tagged recombinant proteins hydrolysed the supercoiled circular plasmid DNA with or without divalent metal ions. The GST-PF3D7_1363500 protein only changed the supercoiled circular plasmid DNA into nicked plasmids, even with Cu2+. Conclusions Fragments derived from five of the endonuclease sequences of P. falciparum 3D7 clone were successfully expressed. The proteins displayed diverse cell distribution, biochemical and enzymatic activities, which indicated that they carried different biological function in the development of the parasite in the erythrocytes. The DNA repair and DNA degradation capacity of the DNA endonucleases in the biology of the parasite remained further studied. Electronic supplementary material The online version of this article (10.1186/s12936-018-2388-0) contains supplementary material, which is available to authorized users.
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- 2018
6. Semaphorin 3A attenuates cardiac autonomic disorders and reduces inducible ventricular arrhythmias in rats with experimental myocardial infarction
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Xinran Li, Wenjuan Cheng, Jie Yin, Yugen Shi, Hesheng Hu, Ye Wang, Na Yang, Mei Xue, Xiaolu Li, Yongli Xuan, and Suhua Yan
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0301 basic medicine ,Tachycardia ,Male ,Sympathetic nervous system ,Sympathetic Nervous System ,Myocardial Infarction ,030204 cardiovascular system & hematology ,Electrocardiography ,Norepinephrine ,0302 clinical medicine ,Myocardial infarction ,Chromatography, High Pressure Liquid ,Heart ,Immunohistochemistry ,Recombinant Proteins ,medicine.anatomical_structure ,Gene Knockdown Techniques ,Ventricular Fibrillation ,Cardiology ,cardiovascular system ,Ventricular arrhythmia ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,Research Article ,Cardiac function curve ,medicine.medical_specialty ,animal structures ,Blotting, Western ,Cardiac autonomic nerve ,Autonomic disorder ,Autonomic Nervous System ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Semaphorin ,Internal medicine ,medicine ,Animals ,cardiovascular diseases ,Semaphorin 3A ,Rats, Wistar ,business.industry ,Myocardium ,Semaphorin-3A ,medicine.disease ,Rats ,Autonomic nervous system ,030104 developmental biology ,Ventricular fibrillation ,Tachycardia, Ventricular ,business - Abstract
Background To investigate the effects of semaphorin 3A (sema 3A) on cardiac autonomic regulation and subsequent ventricular arrhythmias (VAs) in post-infarcted hearts. Method and results In order to explore the functions of sema 3A in post-infarcted hearts, lentivirus-Sema 3A-shRNA and negative control vectors were delivered to the peri-infarcted myocardium rats respectively. Meanwhile, recombinant sema 3A and control (0.9 % NaCl solution) were injected intravenously into infarcted rats to test the therapeutic potential of sema 3A. Results indicated that levels of sema 3A were higher in post-infarcted hearts compared with sham rats. However, sema 3A silencing leaded to sympathetic hyperinnervation, increased myocardial norepinephrine (NE) content and inducible VAs. Conversely, the intravenous administration of sema 3A to infarcted rats reduced sympathetic nerve sprouting, improved cardiac autonomic regulation, and decreased the incidence of inducible VAs. However, both infarct size and cardiac function were similar among infarcted hearts. Conclusions The upregulation and administration of sema 3A exerted beneficial effects on infarction-induced cardiac autonomic disorders by increasing cardiac electrical stability and reducing VAs. Sema 3A might be a potential therapeutic agent for cardiac autonomic abnormalities induced arrhythmias.
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- 2016
7. Expression and functional analysis of the TatD-like DNase of Plasmodium knowlesi.
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Yapan Zhou, Ning Jiang, Xiaoyu Sang, Na Yang, Ying Feng, Qijun Chen, Bo Xiao, and Lubin Jiang
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PLASMODIUM ,SURFACE area ,DNA ,HYDROLYSIS ,MICROBIAL virulence - Abstract
Background: In recent years, human infection by the simian malaria parasite Plasmodium knowlesi has increased in Southeast Asia, leading to growing concerns regarding the cross-species spread of the parasite. Consequently, a deeper understanding of the biology of P. knowlesi is necessary in order to develop tools for control of the emerging disease. TatD-like DNase expressed at the surface of P. falciparum has recently been shown to counteract host innate immunity and is thus a potential malaria vaccine candidate. Methods: The expression of the TatD DNase of P. knowlesi (PkTatD) was confirmed by both Western-blot and immunofluorescent assay. The DNA catalytic function of the PkTatD was confirmed by digestion of DNA with the recombinant PkTatD protein in the presence of various irons. Results: In the present study, we investigated the expression of the homologous DNase in P. knowlesi. The expression of TatD-like DNase in P. knowslesi (PkTatD) was verified by Western blot and indirect immunofluorescence assays. Like that of the P. falciparum parasite, PkTatD was also found to be located on the surface of erythrocytes infected by the parasites. Biochemical analysis indicated that PkTatD can hydrolyze DNA and this activity is magnesium-dependent. Conclusions: We identified that PkTatD expressed on the surface of P. knowlesi-infected RBCs is a Mg
2+ -dependent DNase and exhibits a stronger hydrolytic capacity than TatD from P. falciparum. The data support our previous findings that TatD-like DNase is a unanimously expressed virulence factor of Plasmodium parasites. [ABSTRACT FROM AUTHOR]- Published
- 2018
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8. Are there too many screw holes in plates for fracture fixation?
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Hongzhi Lv, Wenli Chang, Peizhi Yuwen, Na Yang, Xiaoli Yan, Yingze Zhang, Lv, Hongzhi, Chang, Wenli, Yuwen, Peizhi, Yang, Na, Yan, Xiaoli, and Zhang, Yingze
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FRACTURE fixation ,ARTIFICIAL implants ,INTERNAL fixation in fractures ,EXTREMITIES (Anatomy) ,SURGEONS ,BONE screws ,BONE fractures ,ORTHOPEDIC implants ,RETROSPECTIVE studies - Abstract
Background: Implant breakage after the fixation of traumatic fractures is rare; however, when it occurs, it is debilitating for the patients and a challenge for surgeons. The purpose of this study was to analyze and identify the independent risk factors for implant breakage of traumatic fractures treated with plate osteosynthesis.Methods: We reviewed the medical records of patients with a fracture to any part of their four extremities, clavicle, hand or foot, who underwent surgical plate osteosynthesis from January 2005 to January 2015, and who sustained a subsequent implant breakage. Kaplan-Meier univariate and multivariate Cox regressions were performed to identify independent associations of potential risk factors for implant breakage in this cohort.Results: We identified 168 patients who underwent plate osteosynthesis surgery and had subsequent internal fixator breakage. The mean patient age was 40.63 ± 16.71 years (range, 3 to 78 years), with 72.0% (121) males and 28.0% (47) females. The average time between surgery and implant breakage was 12.85 ± 12.42 months (range, 1 to 60 months). In the final regression model, we show that inserting screws close to the fracture line is an independent predictive risk factor for implant breakage (HR, 2.165, 95%CI, 1.227 to 3.822; P = 0.008).Conclusions: We found that inserting screws close to the fracture line is related to an increased risk of internal fixator breakage in patients treated with plate osteosynthesis after fracture. Plates with additional holes likely lead to an increased risk of implant breakage, presumably because surgeons cannot resist inserting extra screws into the holes adjacent to the fracture line, which reduces the stiffness of the plate. We have addressed this problem by designing a plate without holes adjacent to the fracture line. [ABSTRACT FROM AUTHOR]- Published
- 2017
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9. Seroprevalence and genotypes of Toxoplasma gondii isolated from pigs intended for human consumption in Liaoning province, northeastern China.
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Dawei Wang, Yan Liu, Tiantian Jiang, Guoxin Zhang, Gaoming Yuan, Jianbin He, Chunlei Su, and Na Yang
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TOXOPLASMA gondii ,SEROPREVALENCE ,GENOTYPES ,SWINE - Abstract
Background: Genetic information for Toxoplasma gondii isolates from pigs in eastern, south, and southwestern regions of China has been reported previously. However, there are no data from pigs in the northeastern area of the country. To better understand the epidemiology of T. gondii, we determined the seroprevalence and genotypes of T. gondii from pigs slaughtered for human consumption in Liaoning province, northeastern China. Findings: Out of 2063 pigs examined, 233 (11.26 %) were seropositive for T. gondii by the modified agglutination test (MAT), and viable parasites were isolated by bioassay in mice from 23 (9.87 %) of the 233 seropositive pigs. Fifteen out of 23 isolates were genotyped using 10 PCR-restriction fragment length polymorphism (RFLP) genetic markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico. One isolate was identified as ToxoDB genotype #3 (type II-variant), and one was genotype #1 or #3. The other 13 isolates were ToxoDB #9 (type Chinese 1). Conclusions: To our knowledge, this is the first report of T. gondii isolation and genotyping from pigs in northeastern China. This study indicates that pigs are a potential source for transmission of T. gondii to humans, therefore poses a potential public health concern. The genotyping results revealed the presence of genotype Chinese 1 in northeastern China, enriching the scope of T. gondii genotypes distribution in eastern Asia. [ABSTRACT FROM AUTHOR]
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- 2016
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10. Semaphorin 3A attenuates cardiac autonomic disorders and reduces inducible ventricular arrhythmias in rats with experimental myocardial infarction.
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Hesheng Hu, Yongli Xuan, Mei Xue, Wenjuan Cheng, Ye Wang, Xinran Li, Jie Yin, Xiaolu Li, Na Yang, Yugen Shi, Suhua Yan, Hu, Hesheng, Xuan, Yongli, Xue, Mei, Cheng, Wenjuan, Wang, Ye, Li, Xinran, Yin, Jie, Li, Xiaolu, and Yang, Na
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VENTRICULAR arrhythmia ,MYOCARDIAL infarction complications ,MYOCARDIAL infarction treatment ,SEMAPHORINS ,MICE behavior ,LABORATORY mice ,BLOOD circulation disorders ,INNERVATION of the heart ,HEART metabolism ,PROTEIN metabolism ,ANIMAL experimentation ,ANIMALS ,AUTONOMIC nervous system ,ELECTROCARDIOGRAPHY ,GENETIC techniques ,HEART ,HIGH performance liquid chromatography ,IMMUNOHISTOCHEMISTRY ,MYOCARDIAL infarction ,MYOCARDIUM ,NORADRENALINE ,POLYMERASE chain reaction ,PROTEINS ,RATS ,RECOMBINANT proteins ,SYMPATHETIC nervous system ,VENTRICULAR fibrillation ,VENTRICULAR tachycardia ,WESTERN immunoblotting ,PHARMACODYNAMICS ,INNERVATION - Abstract
Background: To investigate the effects of semaphorin 3A (sema 3A) on cardiac autonomic regulation and subsequent ventricular arrhythmias (VAs) in post-infarcted hearts.Method and Results: In order to explore the functions of sema 3A in post-infarcted hearts, lentivirus-Sema 3A-shRNA and negative control vectors were delivered to the peri-infarcted myocardium rats respectively. Meanwhile, recombinant sema 3A and control (0.9% NaCl solution) were injected intravenously into infarcted rats to test the therapeutic potential of sema 3A. Results indicated that levels of sema 3A were higher in post-infarcted hearts compared with sham rats. However, sema 3A silencing leaded to sympathetic hyperinnervation, increased myocardial norepinephrine (NE) content and inducible VAs. Conversely, the intravenous administration of sema 3A to infarcted rats reduced sympathetic nerve sprouting, improved cardiac autonomic regulation, and decreased the incidence of inducible VAs. However, both infarct size and cardiac function were similar among infarcted hearts.Conclusions: The upregulation and administration of sema 3A exerted beneficial effects on infarction-induced cardiac autonomic disorders by increasing cardiac electrical stability and reducing VAs. Sema 3A might be a potential therapeutic agent for cardiac autonomic abnormalities induced arrhythmias. [ABSTRACT FROM AUTHOR]- Published
- 2016
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11. Two-step synthesis of fatty acid ethyl ester from soybean oil catalyzed by Yarrowia lipolytica lipase
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Ying Li, Yuejuan Li, Xiaomei Liang, Yonghong Meng, Zhiqi Zhou, Jilun Li, Guili Wang, Jinnan Chen, and Na Yang
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Acid value ,food.ingredient ,lcsh:Biotechnology ,Triacylglycerol lipase ,Management, Monitoring, Policy and Law ,Applied Microbiology and Biotechnology ,lcsh:Fuel ,Soybean oil ,chemistry.chemical_compound ,food ,lcsh:TP315-360 ,lcsh:TP248.13-248.65 ,Glycerol ,Lipase ,Biodiesel ,Chromatography ,biology ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Research ,Transesterification ,General Energy ,Biochemistry ,Biodiesel production ,biology.protein ,Biotechnology - Abstract
Background Enzymatic biodiesel production by transesterification in solvent media has been investigated intensively, but glycerol, as a by-product, could block the immobilized enzyme and excess n-hexane, as a solution aid, would reduce the productivity of the enzyme. Esterification, a solvent-free and no-glycerol-release system for biodiesel production, has been developed, and two-step catalysis of soybean oil, hydrolysis followed by esterification, with Yarrowia lipolytica lipase is reported in this paper. Results First, soybean oil was hydrolyzed at 40°C by 100 U of lipase broth per 1 g of oil with approximately 30% to 60% (vol/vol) water. The free fatty acid (FFA) distilled from this hydrolysis mixture was used for the esterification of FFA to fatty acid ethyl ester by immobilized lipase. A mixture of 2.82 g of FFA and equimolar ethanol (addition in three steps) were shaken at 30°C with 18 U of lipase per 1 gram of FFA. The degree of esterification reached 85% after 3 hours. The lipase membranes were taken out, dehydrated and subjected to fresh esterification so that over 82% of esterification was maintained, even though the esterification was repeated every 3 hours for 25 batches. Conclusion The two-step enzymatic process without glycerol released and solvent-free demonstrated higher efficiency and safety than enzymatic transesterification, which seems very promising for lipase-catalyzed, large-scale production of biodiesel, especially from high acid value waste oil.
- Published
- 2011
12. Variant RONΔ160 of the RON receptor tyrosine kinase promotes the growth and invasion in vitro and in vivo in gastric cancer cell lines.
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Dong-Hui Zhou, Chao Li, and Li-Na Yang
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PROTEIN-tyrosine kinases ,AMINO acids ,ONCOLOGY ,CARCINOGENS ,CANCER cells - Abstract
Background: Recepteur d'origine nantais (RON) is a receptor tyrosine kinase whose overexpression has been observed in human gastric cancers. This study aimed to determine whether overexpression of the variant RONΔ160 could induce tumorigenicity of gastric cancer cells in vitro or in vivo, and whether its specific small molecule inhibitor (Compound I) could inhibit the effect of RONΔ160. Methods: We constructed human gastric cancer cell line MGC-803 that was stably transfected with a recombinant plasmid expressing RONΔ160, and the effect of RONΔ160 overexpression and macrophage-stimulating protein (MSP) activation on proliferation, migration and invasion abilities of MGC-803 cells were evaluated. Tumor-bearing mice with gastric cancer cells were used to analyze the effects of RONΔ160 overexpression and Compound I on implanted tumor growth. Results: In vitro, overexpression of RONΔ160 in MGC-803 cells resulted changes to their cell morphology, and promoted cell proliferation, migration and invasion. In addition, overexpression of RONΔ160 increased the proportion of cells in the S phase. The effect of RONΔ160 was significantly enhanced by induction of MSP inducing (p < 0.05). In vivo, RONΔ160 promoted the growth of MGC-803 cells in nude mice, including increased tumor size and weight, and lower tumor incubation period. The Compound I inhibited the tumorigenic abilities of RONΔ160 (p <0.05). Conclusions: The results indicate that overexpression of the variant RONΔ160 altered the phenotype and tumorigenicity of MGC-803 cells. Its specific small molecule inhibitor could inhibit the effect of RONΔ160. Therefore, the variant RONΔ160 may become a potential therapeutic target for gastric cancer. [ABSTRACT FROM AUTHOR]
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- 2015
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13. Common rs7138803 variant of FAIM2 and obesity in Han Chinese.
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Cong Li, Xueping Qiu, Na Yang, Jiajia Gao, Yuan Rong, Chenling Xiong, and Fang Zheng
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OBESITY ,CARDIOVASCULAR diseases ,DIABETES ,SINGLE nucleotide polymorphisms ,HEALTH of Chinese people ,HARDY-Weinberg formula - Abstract
Background: Obesity causes severe healthcare problem worldwide leading to numerous diseases, such as cardiovascular diseases and diabetes mellitus. Previous Genome-Wide Association Study (GWAS) identified an association between a single nucleotide polymorphism (SNP) rs7138803, on chromosome 12q13 and obesity in European Caucasians. Since the genetic architecture governing the obesity may vary among different populations, we investigate the variant rs7138803 in Chinese population to find out whether it is associated with obesity. Methods: A population-based cohort association study was carried out using the High Resolution Melt (HRM) method with 1851 participants. The association between rs7138803 genotypes and body mass index (BMI) was modeled with a general linear model, and a case-control study for the association between rs7138803 genotypes and obesity was performed using Pearson's x
2 test. There was no indication of a deviation from Hardy-Weinberg equilibrium (HWE p value = 0.51) in our sample. Results: No association was detected between SNP rs7138803 and BMI in our Chinese Han population with a P value of 0.51. SNP rs7138803 was found to be not associated with common forms of obesity after adjusting for age and sex in the Chinese population. SNP rs7138803 was not associated with other obesity related traits, including T2DM, hypertension, lipid profiles, and ischemic stroke. Conclusion: Our data suggest that the rs7138803 exerts no significant effect on obesity in Chinese Han population. Larger cohorts may be more appropriate to detect an effect of this SNP on common obesity. [ABSTRACT FROM AUTHOR]- Published
- 2013
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14. Seroprevalence of Toxoplasma gondii in slaughtered horses and donkeys in Liaoning province, northeastern China.
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Na Yang, Ming-Yang Mu, Gao-ming Yuan, Guo-xin Zhang, Hong-Kui Li, and Jian-Bin He
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TOXOPLASMA gondii , *SEROPREVALENCE , *SLAUGHTERING , *HORSES , *DONKEYS - Abstract
Background: Toxoplasma gondii is an important zoonotic pathogen infecting humans and almost all warm-blooded animals. The most common sources of human infection are ingestion of tissue cysts in raw or undercooked meat. However, limited information is available about T. gondii infection in horses and donkeys in China. In the present study, we report the seroprevalence of T. gondii infection in horses and donkeys in Liaoning province, northeastern China. Methods: Serum samples were collected from 711 and 738 slaughtered horses and donkeys, respectively, in 13 regions of Liaoning province. The modified agglutination test (MAT) was used to test the specific antibodies to T. gondii. Results: In this study, 178 of 711 (25.0%) horses were seropositive for T. gondii with titers of 1:25 in 81, 1:50 in 62, 1:100 in 33, and 1:200 in 2, and seroprevalence of T. gondii infection from 13 regions ranged from 8.2% to 37.0%. Antibodies to T. gondii were found in 174 of 738 (23.6%) donkeys with titers of 1:25 in 66, 1:50 in 54, 1:100 in 49, and 1:200 in 5, and seroprevalence varied in 13 different regions, ranging from 9.1% to 32.6%. Conclusions: The results of the present study indicated that the rate of infection with T. gondii in horses and donkeys is a little high in Liaoning province, northeastern China in comparison to other surveys in China, which suggests that consumption of horse and donkey meat in this area may represent a potential source for human infection with T. gondii. [ABSTRACT FROM AUTHOR]
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- 2013
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15. Seroprevalence of Toxoplasma gondii infection in slaughtered chickens, ducks, and geese in Shenyang, northeastern China.
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Na Yang, Ming-Yang Mu, Hong-Kui Li, Miao Long, and Jian-Bin He
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TOXOPLASMA gondii , *FRESHWATER animals , *CHICKENS , *DUCKS , *POULTRY , *TOXOPLASMA - Abstract
Background: In recent years, investigations of Toxoplasma gondii infection in poultry (chickens, ducks, and geese) have been reported worldwide, including China. However, little is known about the prevalence of T. gondii infection in poultry in northeastern China. Therefore, the present study was performed to determine the prevalence of T. gondii infection in slaughtered chickens, ducks, and geese in Shenyang, northeastern China. Methods: In the present study, the seroprevalence of T. gondii in 502 adult chickens, 268 adult ducks, and 128 adult geese was surveyed using the modified agglutination test (MAT). Results: The seroprevalences of T. gondii were 5.8%, 7.8%, and 4.7% in chickens, ducks, and geese, respectively. Prevalence was higher in free-range groups (11.2%, 12.3%, and 8.9%) than caged groups (4.7%, 7.5%, and 6.0%), and there was a statistically significant difference only between free-range chickens and caged chickens, but no significant difference was found between free-range ducks, geese and caged ducks, geese. Conclusions: The present study shows the prescence of T. gondii infection in slaughtered chickens, ducks, and geese in Shenyang, northeastern China, which suggests that consumption of poultry meat in Shenyang may pose a potential threat to human health and should be given attention. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
16. Two-step synthesis of fatty acid ethyl ester from soybean oil catalyzed by Yarrowia lipolytica lipase.
- Author
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Yonghong Meng, Guili Wang, Na Yang, Zhiqi Zhou, Yuejuan Li, Xiaomei Liang, Jinnan Chen, Ying Li, and Jilun Li
- Subjects
BIODIESEL fuels ,TRANSESTERIFICATION ,GLYCERIN ,SOY oil ,LIPASES ,ESTERS - Abstract
Background: Enzymatic biodiesel production by transesterification in solvent media has been investigated intensively, but glycerol, as a by-product, could block the immobilized enzyme and excess n-hexane, as a solution aid, would reduce the productivity of the enzyme. Esterification, a solvent-free and no-glycerol-release system for biodiesel production, has been developed, and two-step catalysis of soybean oil, hydrolysis followed by esterification, with Yarrowia lipolytica lipase is reported in this paper. Results: First, soybean oil was hydrolyzed at 40°C by 100 U of lipase broth per 1 g of oil with approximately 30% to 60% (vol/vol) water. The free fatty acid (FFA) distilled from this hydrolysis mixture was used for the esterification of FFA to fatty acid ethyl ester by immobilized lipase. A mixture of 2.82 g of FFA and equimolar ethanol (addition in three steps) were shaken at 30°C with 18 U of lipase per 1 gram of FFA. The degree of esterification reached 85% after 3 hours. The lipase membranes were taken out, dehydrated and subjected to fresh esterification so that over 82% of esterification was maintained, even though the esterification was repeated every 3 hours for 25 batches. Conclusion: The two-step enzymatic process without glycerol released and solvent-free demonstrated higher efficiency and safety than enzymatic transesterification, which seems very promising for lipase-catalyzed, large-scale production of biodiesel, especially from high acid value waste oil. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
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