37 results on '"Ping Wei"'
Search Results
2. Simultaneous resection of coexisting pulmonary and mediastinal lesions by video-assisted thoracic surgery: a case-series study
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Zhang, Jiaheng, Gao, Yi, Zou, Wenbing, Ping, Wei, Zhu, Yunpeng, Fu, Xiangning, and Fu, Shengling
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- 2022
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3. DEPDC1B collaborates with GABRD to regulate ESCC progression
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Yuan, Yunfeng, Ping, Wei, Zhang, Ruijie, Hao, Zhipeng, and Zhang, Ni
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- 2022
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4. Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae
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Ho, Ping-Wei, Piampongsant, Supinya, Gallone, Brigida, Del Cortona, Andrea, Peeters, Pieter-Jan, Reijbroek, Frank, Verbaet, Jules, Herrera, Beatriz, Cortebeeck, Jeroen, Nolmans, Robbe, Saels, Veerle, Steensels, Jan, Jarosz, Daniel F., and Verstrepen, Kevin J.
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- 2021
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- View/download PDF
5. A new minimally invasive technique for correction of pectus carinatum
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Ping, Wei, Fu, Shengling, Li, Yangkai, Yu, Jun, Zhang, Ni, Fu, Xiangning, and Cai, Yixin
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- 2021
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- View/download PDF
6. Surgical outcomes of one-stage resection for synchronous multiple primary lung adenocarcinomas with no less than three lesions
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Qu, Rirong, Tu, Dehao, Ping, Wei, Cai, Yixin, Zhang, Ni, and Fu, Xiangning
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- 2021
- Full Text
- View/download PDF
7. Correction to: Tumor-derived exosomal miR-934 induces macrophage M2 polarization to promote liver metastasis of colorectal cancer
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Dawei Li, Zhiyu Chen, Xuefeng He, Bingjie Guan, Ye Xu, Xinyang Zhong, Zhengxiang Zhang, Senlin Zhao, Binbin Zheng, Sanjun Cai, Guichao Li, Ping Wei, Yanzi Gu, Yushuai Mi, and Xinxiang Li
- Subjects
Cancer Research ,medicine.medical_specialty ,Colorectal cancer ,Exosomes ,lcsh:RC254-282 ,Metastasis ,Text mining ,Internal medicine ,medicine ,Macrophage ,Humans ,Molecular Biology ,Hematology ,lcsh:RC633-647.5 ,business.industry ,Macrophages ,Liver Neoplasms ,Correction ,lcsh:Diseases of the blood and blood-forming organs ,M2 polarization ,Tumor-Derived ,Macrophage Activation ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Up-Regulation ,MicroRNAs ,Oncology ,Cancer research ,business ,Colorectal Neoplasms - Abstract
Mounting evidence has demonstrated the vital importance of tumor-associated macrophages (TAMs) and exosomes in the formation of the premetastatic niche. However, the molecular mechanisms by which tumor-derived exosomal miRNAs interact with TAMs underlying premetastatic niche formation and colorectal cancer liver metastasis (CRLM) remain largely unknown.Transmission electron microscopy and differential ultracentrifugation were used to verify the existence of exosomes. In vivo and in vitro assays were used to identify roles of exosomal miR-934. RNA pull-down assay, dual-luciferase reporter assay, etc. were applied to clarify the mechanism of exosomal miR-934 regulated the crosstalk between CRC cells and M2 macrophages.In the present study, we first demonstrated the aberrant overexpression of miR-934 in colorectal cancer (CRC), especially in CRLM, and its correlation with the poor prognosis of CRC patients. Then, we verified that CRC cell-derived exosomal miR-934 induced M2 macrophage polarization by downregulating PTEN expression and activating the PI3K/AKT signaling pathway. Moreover, we revealed that hnRNPA2B1 mediated miR-934 packaging into exosomes of CRC cells and then transferred exosomal miR-934 into macrophages. Interestingly, polarized M2 macrophages could induce premetastatic niche formation and promote CRLM by secreting CXCL13, which activated a CXCL13/CXCR5/NFκB/p65/miR-934 positive feedback loop in CRC cells.These findings indicate that tumor-derived exosomal miR-934 can promote CRLM by regulating the crosstalk between CRC cells and TAMs. These findings reveal a tumor and TAM interaction in the metastatic microenvironment mediated by tumor-derived exosomes that affects CRLM. The present study also provides a theoretical basis for secondary liver cancer.
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- 2021
8. Microbial fuel cell-assisted utilization of glycerol for succinate production by mutant of Actinobacillus succinogenes
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Ping Wei, Tianwen Zheng, Fengxue Xin, Yaliang Ji, Bin Xu, Wenming Zhang, Weiliang Dong, Jiangfeng Ma, and Min Jiang
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0106 biological sciences ,Neutral red ,Microbial fuel cell ,Succinate ,lcsh:Biotechnology ,Management, Monitoring, Policy and Law ,01 natural sciences ,Applied Microbiology and Biotechnology ,lcsh:Fuel ,Glycerol utilization ,03 medical and health sciences ,chemistry.chemical_compound ,lcsh:TP315-360 ,010608 biotechnology ,lcsh:TP248.13-248.65 ,Glycerol ,Food science ,Actinobacillus succinogenes ,ARTP mutagenesis ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Biodiesel ,biology ,Renewable Energy, Sustainability and the Environment ,Research ,Membrane transport ,Electron acceptor ,biology.organism_classification ,General Energy ,chemistry ,NAD+ kinase ,Biotechnology - Abstract
Background The global production of glycerol is increasing year by year since the demands of biodiesel is rising. It is benefit for high-yield succinate synthesis due to its high reducing property. A. succinogenes, a succinate-producing candidate, cannot grow on glycerol anaerobically, as it needs a terminal electron acceptor to maintain the balance of intracellular NADH and NAD+. Microbial fuel cell (MFC) has been widely used to release extra intracellular electrons. However, A. succinogenes is a non-electroactive strain which need the support of electron shuttle in MFC, and pervious research showed that acid-tolerant A. succinogenes has higher content of unsaturated fatty acids, which may be beneficial for the transmembrane transport of lipophilic electron shuttle. Results MFC-assisted succinate production was evaluated using neutral red as an electron shuttle to recover the glycerol utilization. First, an acid-tolerant mutant JF1315 was selected by atmospheric and room temperature plasma (ARTP) mutagenesis aiming to improve transmembrane transport of neutral red (NR). Additionally, MFC was established to increase the ratio of oxidized NR to reduced NR. By combining these two strategies, ability of JF1315 for glycerol utilization was significantly enhanced, and 23.92 g/L succinate was accumulated with a yield of 0.88 g/g from around 30 g/L initial glycerol, along with an output voltage above 300 mV. Conclusions A novel MFC-assisted system was established to improve glycerol utilization by A. succinogenes for succinate and electricity production, making this system as a platform for chemicals production and electrical supply simultaneously.
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- 2021
9. Isobavachalcone inhibits Pseudorabies virus by impairing virus-induced cell-to-cell fusion
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Tong-Yun Wang, Yan-Dong Tang, Yu Wang, Ping Wei, and Tian-Xin Liu
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0301 basic medicine ,Swine ,animal diseases ,viruses ,030106 microbiology ,Cell ,Short Report ,Pseudorabies ,Kidney ,Virus Replication ,Antiviral Agents ,Virus ,lcsh:Infectious and parasitic diseases ,PRV ,Cell Line ,Cell Fusion ,03 medical and health sciences ,Chalcones ,In vivo ,Virology ,medicine ,Animals ,lcsh:RC109-216 ,Antiviral ,skin and connective tissue diseases ,Fusion ,Cell fusion ,biology ,RNA virus ,biology.organism_classification ,Porcine reproductive and respiratory syndrome virus ,Herpesvirus 1, Suid ,030104 developmental biology ,Infectious Diseases ,medicine.anatomical_structure ,Viral replication ,Isobavachalcone - Abstract
Pseudorabies virus (PRV) is an important pathogen that threatens the global swine industry. Currently, there is no effective drug that can clinically prevent or treat PRV infections. Isobavachalcone (IBC), a natural chalcone compound derived from Psoralea corylifolia, displays multiple biological activities, such as antibacterial, antifungal, and anticancer activities. Recently, it was found that IBC exhibited antiviral activity against an RNA virus, porcine reproductive and respiratory syndrome virus (PRRSV), in vitro. In the current study, we further demonstrated for the first time that IBC has a strong inhibitory effect on PRV. Through a viral luciferase expression assay, we showed that the inhibition step occurs mainly in the late stage of viral replication. Finally, via a cell-to-cell fusion assay, we demonstrated that IBC inhibits PRV by blocking virus-mediated cell fusion. Thus, IBC may be a candidate for further therapeutic evaluation against PRV infection in vivo.
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- 2020
10. Simultaneous occurrence of splenic diffuse large B cell lymphoma and gastrointestinal stromal tumor in the stomach: a case report
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Guang-Zhong Yang, Yuan Jian, Ying Wang, Jing Chang, Qian-Mei Sun, Ping Wei, Xiang-Yang Fang, and Qing Chen
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Pathology ,medicine.medical_specialty ,Histology ,Lymphoma ,Gastrointestinal Stromal Tumors ,medicine.medical_treatment ,Biopsy ,Splenectomy ,Spleen ,Case Report ,Malignancy ,Pathology and Forensic Medicine ,Neoplasms, Multiple Primary ,03 medical and health sciences ,0302 clinical medicine ,Stomach Neoplasms ,Positron Emission Tomography Computed Tomography ,medicine ,lcsh:Pathology ,Biomarkers, Tumor ,Humans ,Stromal tumor ,Lymph node ,Aged ,business.industry ,Splenic Neoplasms ,General Medicine ,Diffuse large B-cell lymphoma ,medicine.disease ,Immunohistochemistry ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,Lymphoma, Large B-Cell, Diffuse ,Gastrointestinal stromal tumor ,Splenic Lymphoma ,business ,lcsh:RB1-214 - Abstract
Background Although the primary malignant spleen tumor is relatively rare, lymphoma is the most common splenic malignancy. It can have quite different clinical manifestations that usually lead to relatively poor outcomes, and thus early and accurate diagnosis are of utmost importance. Case presentation The present study reports a case of a 67-year-old female with high fever, abnormal spleen (diagnosed by PET/CT) and no obvious lymph node enlargement. After being subjected to splenectomy, the patient was diagnosed with splenic diffuse large B cell lymphoma coexisting with gastrointestinal stromal tumor in the stomach. Conclusions To our knowledge, splenic lymphoma accompanied by gastrointestinal stromal tumor in the stomach is rarely reported. This case report discusses the diagnosis and case management of a patient referring to the existing literature.
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- 2018
11. Knockdown of five trehalase genes using RNA interference regulates the gene expression of the chitin biosynthesis pathway in Tribolium castaneum
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Mengmeng Yang, Shigui Wang, Lina Zhao, Bin Tang, Qida Shen, Guoqiang Xie, Zuo-Kun Shi, and Ping Wei
- Subjects
0106 biological sciences ,0301 basic medicine ,Chitin ,Biology ,01 natural sciences ,Chitin biosynthesis pathway ,03 medical and health sciences ,Tribolium castaneum ,RNA interference ,Multienzyme Complexes ,Gene expression ,Gene silencing ,Animals ,Trehalase ,Gene ,Regulation of gene expression ,Gene knockdown ,Tribolium ,fungi ,Phenotype ,Molecular biology ,Cell biology ,010602 entomology ,030104 developmental biology ,Gene Expression Regulation ,Metabolic Engineering ,Gene Knockdown Techniques ,Gene function ,Biotechnology ,Research Article ,Signal Transduction - Abstract
Background RNA interference is a very effective approach for studies on gene function and may be an efficient method for controlling pests. Trehalase is a key gene in the chitin biosynthesis pathway in insects. Five trehalase genes have been cloned in Tribolium castaneum, though it is not known whether the detailed functions of these trehalases can be targeted for pest control. Results The functions of all five trehalase genes were studied using RNAi, and the most important results showed that the expression of all 12 genes decreased significantly from 12 to 72 h compared with the control groups, except GP1 at 72 h, when the expression of the TcTre2 gene was suppressed. The results also revealed different abnormal phenotypes, and the observed mortality rates ranged from 17 to 42 %. The qRT-PCR results showed that the expression of TPS, GS, two GP, CHS1a and CHS1b genes decreased significantly, while that of the CHS2 gene decreased or increased after RNAi after the five trehalases were silenced at 48 h. In addition, TPS gene expression decreased from 12 to 72 h after dsTcTre injection. Conclusions These results demonstrate that silencing of any individual trehalase gene, especially Tre1-4 and Tre2 gene can lead to moulting deformities and a high mortality rate through the regulation of gene expression in the chitin biosynthesis pathway and may be a potential approach for pest control in the future. Electronic supplementary material The online version of this article (doi:10.1186/s12896-016-0297-2) contains supplementary material, which is available to authorized users.
- Published
- 2016
12. BRCA1-associated protein 1 deficiency in lung adenocarcinoma predicts poor outcome and increased tumor invasion
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Ping Wei, Xiang Du, Chen Shen, and Yiqin Wang
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0301 basic medicine ,Oncology ,Lung adenocarcinoma ,Male ,Cancer Research ,Lung Neoplasms ,Somatic cell ,medicine.disease_cause ,Immunoenzyme Techniques ,0302 clinical medicine ,Invasion ,Cell Movement ,Carcinoma, Non-Small-Cell Lung ,Gene expression ,BAP1 ,Tumor suppressor ,Cell cycle ,Middle Aged ,Prognosis ,Gene Expression Regulation, Neoplastic ,Survival Rate ,Cell Transformation, Neoplastic ,030220 oncology & carcinogenesis ,Lymphatic Metastasis ,Adenocarcinoma ,Immunohistochemistry ,Female ,Ubiquitin Thiolesterase ,Research Article ,Adult ,medicine.medical_specialty ,03 medical and health sciences ,Internal medicine ,Genetics ,medicine ,Biomarkers, Tumor ,Humans ,Neoplasm Invasiveness ,Survival rate ,Aged ,Neoplasm Staging ,business.industry ,Tumor Suppressor Proteins ,Cell Cycle Checkpoints ,medicine.disease ,030104 developmental biology ,Neoplasm Recurrence, Local ,business ,Carcinogenesis ,Follow-Up Studies - Abstract
Background The major pathological type of non-small cell lung cancer is lung adenocarcinoma (LAC), which has a poor prognosis. BRCA1-associated protein-1 (BAP1) is a newly identified tumor suppressor that regulates a number of cellular functions in somatic malignancies. However, the impact of BAP1 expression in LAC has not been investigated. Methods A total of 112 cases of LAC and 101 cases of non-neoplastic lung diseases were included in this study. The study focused on BAP1 expression in lung tissues and its relationship to patients’ clinical and pathological features. BAP1 expression was detected by immunohistochemistry. A human LAC cell line NCI-H1299 was transfected with lipofectamine p3xFLAG-BAP1. BAP1 gene expression was silenced in another LAC cell line NCI-H1650, in order to test the inhibitory effect of BAP1 on cell migration and invasion, as well as cell cycle regulation. Results BAP1 expression showed a negative correlation with tumorigenesis of LAC (p
- Published
- 2016
13. Effects of long-term use of macrolides in patients with non-cystic fibrosis bronchiectasis: a meta-analysis of randomized controlled trials
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Jin-Fu Xu, Shuo Liang, Ping Wei, Hai-Wen Lu, Li Chao Fan, and Xiao Bin Ji
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Diarrhea ,Male ,medicine.medical_specialty ,law.invention ,Randomized controlled trial ,Quality of life ,law ,Fibrosis ,Internal medicine ,Medicine ,Humans ,In patient ,Intensive care medicine ,Randomized Controlled Trials as Topic ,Bronchiectasis ,business.industry ,Non cystic fibrosis bronchiectasis ,medicine.disease ,Infectious Diseases ,Meta-analysis ,Quality of Life ,Female ,Macrolides ,business ,Research Article - Abstract
Background The purpose of this study was to evaluate the clinical benefits and safety of the long-term use of macrolides in patients with non-cystic fibrosis (non-CF) bronchiectasis. Methods Embase, Pubmed, the Cochrane Library and Web of Science databases were searched from inception up to March 2014. The primary outcome was the improvement of exacerbations of bronchiectasis. Secondary endpoints included changes of microbiology, lung function, quality of life, sputum volume, adverse events and macrolide resistance. Results The literature search yielded 139 studies, ten of which containing 601 patients were included in this meta-analysis. Macrolides showed a statistically-significant improvement in reducing acute exacerbations per patient during follow-up treatment (RR = 0.55, 95% CI: 0.47, 0.64, P
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- 2015
14. Patient-derived xenograft in zebrafish embryos: a new platform for translational research in gastric cancer.
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Jia-Qi Wu, Jing Zhai, Chong-Yong Li, Ai-Min Tan, Ping Wei, Li-Zong Shen, and Ming-Fang He
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STOMACH cancer treatment ,GENETIC translation ,XENOGRAFTS ,CELL proliferation ,CANCER chemotherapy ,LABORATORY zebrafish - Abstract
Background: Gastric cancer (GC) is among the most commonly cancer occurred in Asian, especially in China. With its high heterogeneity and few of validated drug targets, GC remains to be one of the most under explored areas of precision medicine. In this study, we aimed to establish an in vivo patient-derived xenograft (PDX) model based on zebrafish (Danio rerio) embryos, allowing for a rapid analysis of the angiogenic and invasive potentials, as well as a fast drug sensitivity testing. Methods: Two human gastric cancer cell lines (AGS and SGC-7901) were xenografted into zebrafish embryos, their sensitivity to 5-FU were tested both in vitro and in vivo. Fourteen human primary cells from gastric cancer tissue were xenografted into zebrafish embryos, their proliferating, angiogenic and metastatic activities were evaluated in vivo. Sensitivity to 5-FU, docetaxel, and apatinib were also tested on primary samples from four patients. Results: SGC-7901 showed higher sensitivity to 5-FU than AGS both in vitro (6.3 ± 0.9 μM vs.10.5 ± 1.8 μM) and in vivo. Nine out of fourteen patient samples were successfully transplanted in zebrafish embryos and all showed proliferating, angiogenic and metastatic potentials in the living embryos. Four cases showed varied sensitivity to the selected three chemotherapeutic drugs. Conclusions: Our zebrafish PDX (zPDX) model is a preclinically reliable in vivo model for GC. The zPDX model is also a promising platform for the translational research and personalized treatment on GC. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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15. Improvement of yeast tolerance to acetic acid through Haa1 transcription factor engineering: towards the underlying mechanisms.
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Swinnen, Steve, Henriques, Sílvia F., Shrestha, Ranjan, Ping-Wei Ho, Sá-Correia, Isabel, and Nevoigt, Elke
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YEAST ,ACETIC acid ,GENETIC transcription ,GENETIC engineering ,SACCHAROMYCES cerevisiae ,ALLELES ,AMINO acids - Abstract
Background: Besides being a major regulator of the response to acetic acid in Saccharomyces cerevisiae, the transcription factor Haa1 is an important determinant of the tolerance to this acid. The engineering of Haa1 either by overexpression or mutagenesis has therefore been considered to be a promising avenue towards the construction of more robust strains with improved acetic acid tolerance. Results: By applying the concept of global transcription machinery engineering to the regulon-specific transcription factor Haa1, a mutant allele containing two point mutations could be selected that resulted in a significantly higher acetic acid tolerance as compared to the wild-type allele. The level of improvement obtained was comparable to the level obtained by overexpression of HAA1, which was achieved by introduction of a second copy of the native HAA1 gene. Dissection of the contribution of the two point mutations to the phenotype showed that the major improvement was caused by an amino acid exchange at position 135 (serine to phenylalanine). In order to further study the mechanisms underlying the tolerance phenotype, Haa1 translocation and transcriptional activation of Haa1 target genes was compared between Haa1 mutant, overproduction and wild-type strains. While the rapid Haa1 translocation from the cytosol to the nucleus in response to acetic acid was not affected in the Haa1
S135F mutant strain, the levels of transcriptional activation of four selected Haa1-target genes by acetic acid were significantly higher in cells of the mutant strain as compared to cells of the wild-type strain. Interestingly, the time-course of transcriptional activation in response to acetic acid was comparable for the mutant and wild-type strain whereas the maximum mRNA levels obtained correlate with each strain's tolerance level. Conclusion: Our data confirms that engineering of the regulon-specific transcription factor Haa1 allows the improvement of acetic acid tolerance in S. cerevisiae. It was also shown that the beneficial S135F mutation identified in the current work did not lead to an increase of HAA1 transcript level, suggesting that an altered protein structure of the Haa1S135F mutant protein led to an increased recruitment of the transcription machinery to Haa1 target genes. [ABSTRACT FROM AUTHOR]- Published
- 2017
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- View/download PDF
16. The sole introduction of two single-point mutations establishes glycerol utilization in Saccharomyces cerevisiae CEN.PK derivatives.
- Author
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Ping-Wei Ho, Swinnen, Steve, Duitama, Jorge, and Nevoigt, Elke
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GLYCERIN derivatives , *SACCHAROMYCES cerevisiae , *BIODIESEL fuels , *NUCLEOTIDE sequencing , *REVERSE engineering - Abstract
Background: Glycerol is an abundant by-product of biodiesel production and has several advantages as a substrate in biotechnological applications. Unfortunately, the popular production host Saccharomyces cerevisiae can barely metabolize glycerol by nature. Results: In this study, two evolved derivatives of the strain CEN.PK113-1A were created that were able to grow in synthetic glycerol medium (strains PW-1 and PW-2). Their growth performances on glycerol were compared with that of the previously published evolved CEN.PK113-7D derivative JL1. As JL1 showed a higher maximum specific growth rate on glycerol (0.164 h-1 compared to 0.119 h-1 for PW-1 and 0.127 h-1 for PW-2), its genomic DNA was subjected to whole-genome resequencing. Two point mutations in the coding sequences of the genes UBR2 and GUT1 were identified to be crucial for growth in synthetic glycerol medium and subsequently verified by reverse engineering of the wild-type strain CEN.PK113-7D. The growth rate of the resulting reverse-engineered strain was 0.130 h-1. Sanger sequencing of the GUT1 and UBR2 alleles of the above-mentioned evolved strains PW-1 and PW-2 also revealed one single-point mutation in these two genes, and both mutations were demonstrated to be also crucial and sufficient for obtaining a maximum specific growth rate on glycerol of ~0.120 h-1. Conclusions: The current work confirmed the importance of UBR2 and GUT1 as targets for establishing glycerol utilization in strains of the CEN.PK family. In addition, it shows that a growth rate on glycerol of 0.130 h-1 can be established in reverse-engineered CEN.PK strains by solely replacing a single amino acid in the coding sequences of both Ubr2 and Gut1. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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17. Knockdown of five trehalase genes using RNA interference regulates the gene expression of the chitin biosynthesis pathway in Tribolium castaneum.
- Author
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Bin Tang, Ping Wei, Lina Zhao, Zuokun Shi, Qida Shen, Mengmeng Yang, Guoqiang Xie, and Shigui Wang
- Subjects
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RNA interference , *CHITIN biodegradation , *GENE expression , *DNA analysis , *BIOSYNTHESIS , *PEST control - Abstract
Background: RNA interference is a very effective approach for studies on gene function and may be an efficient method for controlling pests. Trehalase is a key gene in the chitin biosynthesis pathway in insects. Five trehalase genes have been cloned in Tribolium castaneum, though it is not known whether the detailed functions of these trehalases can be targeted for pest control. Results: The functions of all five trehalase genes were studied using RNAi, and the most important results showed that the expression of all 12 genes decreased significantly from 12 to 72 h compared with the control groups, except GP1 at 72 h, when the expression of the TcTre2 gene was suppressed. The results also revealed different abnormal phenotypes, and the observed mortality rates ranged from 17 to 42 %. The qRT-PCR results showed that the expression of TPS, GS, two GP, CHS1a and CHS1b genes decreased significantly, while that of the CHS2 gene decreased or increased after RNAi after the five trehalases were silenced at 48 h. In addition, TPS gene expression decreased from 12 to 72 h after dsTcTre injection. Conclusions: These results demonstrate that silencing of any individual trehalase gene, especially Tre1-4 and Tre2 gene can lead to moulting deformities and a high mortality rate through the regulation of gene expression in the chitin biosynthesis pathway and may be a potential approach for pest control in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
18. BRCA1-associated protein 1 deficiency in lung adenocarcinoma predicts poor outcome and increased tumor invasion.
- Author
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Chen Shen, Yiqin Wang, Ping Wei, Xiang Du, Shen, Chen, Wang, Yiqin, Wei, Ping, and Du, Xiang
- Subjects
BRCA proteins ,ADENOCARCINOMA ,CANCER invasiveness ,CELL lines ,NEOPLASTIC cell transformation ,TUMOR suppressor proteins ,PROTEIN metabolism ,CANCER relapse ,CELL cycle ,CELL motility ,ESTERASES ,GENES ,IMMUNOENZYME technique ,LONGITUDINAL method ,LUNG cancer ,LUNG tumors ,METASTASIS ,PROGNOSIS ,PROTEINS ,SURVIVAL ,TUMOR classification ,METABOLISM - Abstract
Background: The major pathological type of non-small cell lung cancer is lung adenocarcinoma (LAC), which has a poor prognosis. BRCA1-associated protein-1 (BAP1) is a newly identified tumor suppressor that regulates a number of cellular functions in somatic malignancies. However, the impact of BAP1 expression in LAC has not been investigated.Methods: A total of 112 cases of LAC and 101 cases of non-neoplastic lung diseases were included in this study. The study focused on BAP1 expression in lung tissues and its relationship to patients' clinical and pathological features. BAP1 expression was detected by immunohistochemistry. A human LAC cell line NCI-H1299 was transfected with lipofectamine p3xFLAG-BAP1. BAP1 gene expression was silenced in another LAC cell line NCI-H1650, in order to test the inhibitory effect of BAP1 on cell migration and invasion, as well as cell cycle regulation.Results: BAP1 expression showed a negative correlation with tumorigenesis of LAC (p <0.001) and lymph node metastasis (p = 0.010). High expression of BAP1 predicted longer disease free survival (p = 0.040) and overall survival (p = 0.021) of LAC patients. In functional assays, BAP1 was found to inhibit the migration and invasion of LAC cells, and promoted their apoptosis and necrosis.Conclusions: We identify BAP1 as a LAC precursor as well as a robust prognostic indicator in LAC patients. This study provides in vitro rationale for the further investigation of BAP1 in preclinical studies. [ABSTRACT FROM AUTHOR]- Published
- 2016
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- View/download PDF
19. Markedly improving asymmetric oxidation of 1-(4-methoxyphenyl) ethanol with Acetobacter sp. CCTCC M209061 cells by adding deep eutectic solvent in a two-phase system.
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Ping Wei, Jing Liang, Jing Cheng, Min-Hua Zong, and Wen-Yong Lou
- Subjects
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OXIDATION , *ETHANOL , *ACETOBACTER , *EUTECTICS , *SOLVENTS , *INDOLE compounds - Abstract
Background: Enantiopure (S)-1-(4-methoxyphenyl) ethanol {(S)-MOPE} can be employed as an important synthon for the synthesis of cycloalkyl [b] indoles with the treatment function for general allergic response. To date, the biocatalytic resolution of racemic MOPE through asymmetric oxidation in the biphasic system has remained largely unexplored. Additionally, deep eutectic solvents (DESs), as a new class of promising green solvents, have recently gained increasing attention in biocatalysis for their excellent properties and many successful examples in biocatalytic processes. In this study, the biocatalytic asymmetric oxidation of MOPE to get (S)-MOPE using Acetobacter sp. CCTCC M209061 cells was investigated in different two-phase systems, and adding DES in a biphasic system was also explored to further improve the reaction efficiency of the biocatalytic oxidation. Results: Of all the examined water-immiscible organic solvents and ionic liquids (ILs), 1-butyl-3-methylimidazolium hexafluorophoshpate ([C4MIM][PF6]) afforded the best results, and consequently was selected as the second phase of a two-phase system for the asymmetric oxidation of MOPE with immobilized Acetobacter sp. CCTCC M209061 cells. For the reaction performed in the [C4MIM][PF6]/buffer biphasic system, under the optimized conditions, the initial reaction rate, the maximum conversion and the residual substrate e.e. recorded 97.8 μmol/min, 50.5 and >99.9 % after 10 h reaction. Furthermore, adding the DES [ChCl][Gly] (10 %, v/v) to the aqueous phase, the efficiency of the biocatalytic oxidation was rose markedly. The optimal substrate concentration and the initial reaction rate were significantly increased to 80 mmol/L and 124.0 μmol/min, respectively, and the reaction time was shortened to 7 h with 51.3 % conversion. The immobilized cell still retained over 72 % of its initial activity after 9 batches of successive reuse in the [C4MIM][PF6]/[ChCl][Gly]-containing buffer system. Additionally, the efficient biocatalytic process was feasible up to a 500-mL preparative scale. Conclusion: The biocatalytic asymmetric oxidation of MOPE with Acetobacter sp. CCTCC M209061 cells was successfully conducted in the [C4MIM][PF6]-containing biphasic system with high conversion and enantioselectivity, and the reaction efficiency was further enhanced by adding [ChCl][Gly] to the reaction system. The efficient biocatalytic process was promising for the preparation of enantiopure (S)-MOPE. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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20. Rapid detection of the common avian leukosis virus subgroups by real-timeloop-mediated isothermal amplification.
- Author
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Hao Peng, Lili Qin, Yuyu Bi, Peikun Wang, Guangzhen Zou, Jun Li, Yongli Yang, Xingfu Zhong, and Ping Wei
- Subjects
AVIAN leukosis ,POULTRY industry ,POLYMERASE chain reaction ,VIRUS isolation ,DNA primers ,DIAGNOSIS - Abstract
Background: Subgroups A, B, E and J are the major subgroups of avian leukosis virus (ALV) infecting chickens. ALV infection has become endemic in China and has a significant negative effect on the poultry industry. Consequently, there is an urgent need for a specific, sensitive and rapid method for diagnosis and eradication of ALV. Therefore we developed a simple and rapid real-time loop-mediated isothermal amplification (LAMP) reaction for the timely detection of the common ALV subgroups, whereby the amplification can be obtained in 35 min under isothermal conditions at 63 °C, ability to specific, sensitive and rapid detect all the common ALV subgroups. Methods: A set of four specific primers was designed to target the sequences of the pol gene of ALV, and the loop-mediated isothermal amplification (LAMP) assay were developed and compared with PCR and virus isolation methods. Results: The results from specificity of the LAMP assay showed that only target ALVs DNA was amplified. The LAMP assay demonstrated a sensitivity of 20 copies/reaction of ALV DNA, which was 10 times higher than the conventional PCR measurement. To further evaluate the reliability of the method, the assay was evaluated with ALV DNA from a panel of 81 clinical samples suspected of ALV infection. The results verify that the LAMP method was more sensitive than the conventional PCR and virus isolation method. Conclusion: In conclusion, the developed LAMP assay was a simple, inexpensive, sensitive method for the rapid detection of the most common subgroups of ALV, and it provided a useful and practical tool in the eradication program for ALV in the poultry industry. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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21. Low expression of LOC285194 is associated with poor prognosis in colorectal cancer
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Peng Qi, Xiang Du, Cong Tan, Midie Xu, Xiao Yan Zhou, Shu Juan Ni, Ping Wei, and Dan Huang
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Male ,Pathology ,medicine.medical_specialty ,Survival ,Colorectal cancer ,Genetic enhancement ,Kaplan-Meier Estimate ,Real-Time Polymerase Chain Reaction ,General Biochemistry, Genetics and Molecular Biology ,Disease-Free Survival ,law.invention ,Intestinal mucosa ,law ,Cell Line, Tumor ,Medicine ,Humans ,Intestinal Mucosa ,Polymerase chain reaction ,Medicine(all) ,Regulation of gene expression ,Biochemistry, Genetics and Molecular Biology(all) ,business.industry ,Research ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Gene Expression Regulation, Neoplastic ,Real-time polymerase chain reaction ,Cell culture ,Multivariate Analysis ,Cancer research ,Long non-coding RNAs ,LOC285194 ,Osteosarcoma ,Female ,RNA, Long Noncoding ,business ,Colorectal Neoplasms - Abstract
Background The long non-coding RNAs (lncRNAs) study has gradually become one of the hot topics in the field of RNA biology. One lncRNA which has attracted attention is LOC285194, a lncRNA demonstrated the potential tumor-suppressor role in osteosarcoma. The aim of this study was to examine the expression of LOC285194 in colorectal cancer (CRC) patients and to investigate the relationship between this lncRNA levels and existing clinicopathologic parameters and patient survival. Methods The expression of LOC285194 was detected by quantitative real-time polymerase chain reaction in pairs of tumorous and adjacent normal tissues of 81 colorectal cancer patients with a follow-up of 5 years, as well as in three colorectal cancer cell lines and normal intestinal mucous cell line. Then, we analyzed the potential relationship between this lncRNA levels in tumor tissues and existing clinicopathological features of CRC, and clinical outcome. Results The relative expression levels of LOC285194 was significantly lower in tumor tissues (p p = 0.015), higher tumor stage (p = 0.034), and more distant metastasis (p = 0.046). Kaplan-Meier analysis indicated that patients with low LOC285194 expression had a poor disease free survival (p = 0.010). Moreover, multivariate analysis showed that decreased expression of LOC285194 was an independent predictor of disease-specific survival. Conclusion Our data indicate that LOC285194 might be a novel prognostic indicator in colorectal cancer and may be a potential target for diagnosis and gene therapy.
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- 2013
22. Effects of long-term use of macrolides in patients with non-cystic fibrosis bronchiectasis: a meta-analysis of randomized controlled trials.
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Li-Chao Fan, Hai-Wen Lu, Ping Wei, Xiao-Bin Ji, Shuo Liang, and Jin-Fu Xu
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BRONCHIECTASIS ,MACROLIDE antibiotics ,PHYSIOLOGICAL effects of antibiotics ,QUALITY of life ,SPUTUM ,DRUG resistance in bacteria ,DRUG side effects ,THERAPEUTICS - Abstract
Background: The purpose of this study was to evaluate the clinical benefits and safety of the long-term use of macrolides in patients with non-cystic fibrosis (non-CF) bronchiectasis. Methods: Embase, Pubmed, the Cochrane Library and Web of Science databases were searched from inception up to March 2014. The primary outcome was the improvement of exacerbations of bronchiectasis. Secondary endpoints included changes of microbiology, lung function, quality of life, sputum volume, adverse events and macrolide resistance. Results: The literature search yielded 139 studies, ten of which containing 601 patients were included in this meta-analysis. Macrolides showed a statistically-significant improvement in reducing acute exacerbations per patient during follow-up treatment (RR = 0.55, 95% CI: 0.47, 0.64, P < 0.001), increasing the number of patients free from exacerbations (OR = 2.81, 95% CI: 1.85, 4.26, P < 0.001), and prolonging time to a first exacerbation (HR = 0.38, 95% CI: 0.28, 0.53, P < 0.001). Macrolides maintenance treatment was superior to control with respect to attenuating FEV1 decline (p = 0.02), improving sputum volume (p = 0.009) and SGRQ total scores (p = 0.02), but showed a higher risk of adverse events, especially diarrhea (OR = 5.36; 95% CI: 2.06, 13.98, P = 0.0006). Eradication of pathogens was improved in the macrolide group (OR = 1.76, 95% CI: 0.91, 3.41, P = 0.09), while pathogen resistance caused by macrolides dramatically increased (OR = 16.83, 95% CI: 7.26, 38.99, P< 0.001). The new appearance of a microbiologic profile or participant withdrawal due to adverse events showed no significant differences between the two groups. Conclusion: In patients with non-CF bronchiectasis, macrolide maintenance treatment can effectively reduce frequency of exacerbations, attenuate lung function decline, decrease sputum volume, improve quality of life, but may be accompanied with increased adverse events (especially diarrhea) and pathogen resistance. [ABSTRACT FROM AUTHOR]
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- 2015
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23. A molecular signature for the prediction of recurrence in colorectal cancer.
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Lisha Wang, Xiaohan Shen, Zhimin Wang, Xiuying Xiao, Ping Wei, Qifeng Wang, Fei Ren, Yiqin Wang, Zebing Liu, Weiqi Sheng, Wei Huang, Xiaoyan Zhou, and Xiang Du
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COLON cancer prognosis ,HEALTH risk assessment ,COLON cancer patients ,GENE expression ,MULTIVARIATE analysis - Abstract
Background: Several clinical and pathological factors have an impact on the prognosis of colorectal cancer (CRC), but they are not yet adequate for risk assessment. We aimed to identify a molecular signature that can reliably identify CRC patients at high risk for recurrence. Results: Two hundred eighty-one CRC samples (stage II/III) were included in this study. A two-step gene expression profiling study was conducted. First, gene expression measurements from 81 fresh frozen CRC samples were obtained using Affymetrix Human Genome U133 Plus 2.0 Arrays. Second, a focused gene expression assay, including prognostic genes and genes of interest from literature reviews, was performed using 200 fresh frozen samples and a Taqman low-density array (TLDA) analysis. An optimal 31-gene expression classifier for the prediction of recurrence among patients with stage II/III CRC was developed using logistic regression analysis. This gene expression signature classified 58.5% of patients as low-risk and 41.5% as high-risk (P < 0.001). The signature was the strongest independent prognostic factor in the multivariate analysis. The five-year relapse-free survival (RFS) rates for the low-risk patients and the high-risk patients were 88.5% and 41.3% (P < 0.001), respectively. Conclusion: We identified a 31-gene expression signature that is closely associated with the clinical outcome of stage II/III CRC patients. [ABSTRACT FROM AUTHOR]
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- 2015
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24. Biocatalytic anti-Prelog reduction of prochiral ketones with whole cells of Acetobacter pasteurianus GIM1.158.
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Peng-Xuan Du, Ping Wei, Wen-Yong Lou, and Min-Hua Zong
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KETONES , *ALCOHOLS (Chemical class) , *ACETOBACTER pasteurianus , *AGRICULTURAL chemicals , *BIOCATALYSIS - Abstract
Background Enantiomerically pure alcohols are important building blocks for production of chiral pharmaceuticals, flavors, agrochemicals and functional materials and appropriate whole-cell biocatalysts offer a highly enantioselective, minimally polluting route to these valuable compounds. At present, most of these biocatalysts follow Prelog's rule, and thus the (S)- alcohols are usually obtained when the smaller substituent of the ketone has the lower CIP priority. Only a few anti-Prelog (R)-specific whole cell biocatalysts have been reported. In this paper, the biocatalytic anti-Prelog reduction of 2-octanone to (R)-2-octanol was successfully conducted with high enantioselectivity using whole cells of Acetobacter pasteurianus GIM1.158. Results Compared with other microorganisms investigated, Acetobacter pasteurianus GIM1.158 was shown to be more effective for the reduction reaction, affording much higher yield, product enantiomeric excess (e.e.) and initial reaction rate. The optimal temperature, buffer pH, cosubstrate and its concentration, substrate concentration, cell concentration and shaking rate were 35°C, 5.0, 500 mmol/L isopropanol, 40 mmol/L, 25 mg/mL and 120 r/min, respectively. Under the optimized conditions, the maximum yield and the product e.e. were 89.5% and >99.9%, respectively, in 70 minutes. Compared with the best available data in aqueous system (yield of 55%), the yield of (R)-2-octanol was greatly increased. Additionally, the efficient whole-cell biocatalytic process was feasible on a 200-mL preparative scale and the chemical yield increased to 95.0% with the product e.e. being >99.9%. Moreover, Acetobacter pasteurianus GIM1.158 cells were proved to be capable of catalyzing the anti- Prelog bioreduction of other prochiral carbonyl compounds with high efficiency. Conclusions Via an effective increase in the maximum yield and the product e.e. with Acetobacter pasteurianus GIM1.158 cells, these results open the way to use of whole cells of this microorganism for challenging enantioselective reduction reactions on laboratory and commercial scales. [ABSTRACT FROM AUTHOR]
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- 2014
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25. Antiviral potency and functional analysis of tetherin orthologues encoded by horse and donkey.
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Xin Yin, Miaomiao Guo, Qinyong Gu, Xingliang Wu, Ping Wei, and Xiaojun Wang
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Background: Tetherin is an interferon-inducible host cell factor that blocks the viral particle release of the enveloped viruses. Most knowledge regarding the interaction between tetherin and viruses has been obtained using the primate lentiviral system. However, much less is known about the functional roles of tetherin on other lentiviruses. Equine infectious anemia virus (EIAV) is an important macrophage-tropic lentivirus that has been widely used as a practical model for investigating the evolution of the host-virus relationship. The host range of EIAV is reported to include all members of the Equidae family. However, EIAV has different clinical responses in horse and donkey. It’s intriguing to investigate the similarities and differences between the tetherin orthologues encoded by horse and donkey. Results: We report here that there are two equine tetherin orthologues. Compared to horse tetherin, there are three valine amino acid deletions within the transmembrane domain and three distinct mutations within the ectodomain of donkey tetherin. However, the antiviral activity of donkey tetherin was not affected by amino acid deletion or substitution. In addition, both tetherin orthologues encoded by horse and donkey are similarly sensitive to EIAV Env protein, and equally activate NF-κB signaling. Conclusion: Our data suggest that both tetherin orthologues encoded by horse and donkey showed similar antiviral activities and abilities to induce NF-κB signaling. In addition, the phenomenon about the differential responses of horses and donkeys to infection with EIAV was not related with the differences in the structure of the corresponding tetherin orthologues. [ABSTRACT FROM AUTHOR]
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- 2014
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26. Serological report of pandemic (H1N1) 2009 infection among cats in Northeastern China in 2012-02 and 2013-03.
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Fu-Rong Zhao, Chun-Guo Liu, Xin Yin, Dong-Hui Zhou, Ping Wei, and Hui-Yun Chang
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H1N1 influenza ,PANDEMICS ,SWINE influenza ,RESPIRATORY infections ,INFLUENZA viruses - Abstract
Background: Influenza A virus has a wide range of hosts. It has not only infected human, but also been reported interspecies transmission from humans to other animals, such as pigs, poultry, dogs and cats. However, prevalence of A (H1N1) pdm09 influenza virus infections in cats in northeastern China is unknown. Therefore, the prevalence of A (H1N1) pdm09 influenza virus infections was performed among cats in northeastern China in this study. Findings: Of all samples in this study, the overall seroprevalence of pandemic (H1N1) 2009 infection in cats was 21% (240/1140). It also showed a higher prevalence rate of pandemic(H1N1) 2009 infection in pet cats (30.6%) than roaming cats (11%) based on NT. In addition, the results also showed a trend of difference in term of species of cats and it was statistically significant. Conclusions: This is the first survey on the seroprevalence of pandemic (H1N1) 2009 infection among cats in northeastern China. This study has observed a relatively high seroprevalence of pandemic (H1N1) 2009 among different cat populations in northeastern China, similar seroprevalence studies should be conducted elsewhere. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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27. Abnormal expression of CDK11p58 in prostate cancer.
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Yayun Chi, Lisha Wang, Xiuying Xiao, Ping Wei, Yiqin Wang, and Xiaoyan Zhou
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PROSTATE cancer ,CELL cycle ,CANCER cells ,APOPTOSIS ,INTEGRINS - Abstract
Background CDK11
p58 is one of the large families of p34cdc2-related kinases whose functions are linked with cell cycle progression, tumorigenesis and apoptotic signaling. Our previous investigation demonstrated that CDK11p58 repressed androgen receptor (AR) transcriptional activity and was involved in the negative regulation of AR function. Methods CDK11p58 expression was examined in the prostate cancer tissues and adjacent tissues by IHC and qRT-PCR. Cell apoptosis was detected by flow cytometry. The metastasis of cancer cells was evaluated by the Transwell Assay. Finally we further investigated the underlying molecular mechanisms by examining expression levels of relevant proteins using western blot analysis. Results We found that both RNA and protein expression of CDK11p58 were low in prostate cancer tissues compared with its adjacent noncancerous tissues. CDK11p58 promoted the prostate cancer cell apoptosis and inhibited its metastasis in a kinase dependent way. And finally CDK11p58 could inhibit the metastasis of AR positive prostate cancer cells through inhibition of integrin β3 and MMP2. Conclusions These data indicate that CDK11p58 is an anti-metastasis gene product in prostate cancer. [ABSTRACT FROM AUTHOR]- Published
- 2014
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28. Frequent copy number variations of PI3K/AKT pathway and aberrant protein expressions of PI3K subunits are associated with inferior survival in diffuse large B cell lymphoma.
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Wenli Cui, Ying Cai, Weige Wang, Zebing Liu, Ping Wei, Rui Bi, Weixiang Chen, Menghong Sun, and Xiaoyan Zhou
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PHOSPHATIDYLINOSITOL 3-kinases ,PHOSPHOINOSITIDES ,B cells ,IMMUNOHISTOCHEMISTRY ,LYMPHOMAS ,LYMPHATIC diseases - Abstract
Background It has been reported that the PI3K/AKT signaling pathway is activated in diffuse large B-cell lymphoma (DLBCL), PI3K constitutive activation plays a crucial role in PI3K/AKT pathway. However, the copy number variations (CNVs) of PI3K subunits on gene level remain unknown in DLBCL. Therefore, the aim of the study is to investigate the CNV of PI3K subunits and their relationship with clinicopathological features exploring the possible mechanism underlying of PI3K activation in DLBCL. Methods CNV of 12 genes in the PI3K/AKT pathway was detected by NanoString nCounter in 60 de novo DLBCLs and 10 reactive hyperplasia specimens as controls. Meanwhile, immunohistochemistry (IHC) was performed to examine the expression of p110α, p110β, p110γ, p110δ, and pAKT on DLBCL tissue microarrays. Results All PI3K and AKT subunits, except for PIK3R1, had various CNVs in the form of copy number amplifications and copy number losses. Their rates were in the range of 8.3-20.0%. Of them PIK3CA and PIK3CB gene CNVs were significantly associated with decreased overall survival (P = 0.029 and P = 0.019, respectively). IHC showed that the frequency of strong positive expression of p110α, p110β, p110γ, and p110δ were 26.7%, 25.0%, 18.3%, and 25.0% respectively, and they were found to be associated with decreased survival (P = 0.022, P = 0.015, P = 0.015, and P = 0.008, respectively). Expression of p110α was not only significantly associated with CNVs of PIK3CA (P = 0.002) but also positively correlated with strong positive expression of pAKT (P = 0.026). Conclusions CNV of PIK3CA is highly associated with aberrant p110α protein expression and subsequent activation of PI3K/AKT pathway. CNVs of PIK3CA and PIK3CB, and aberrant protein expression of p110 isoforms are of great important value for predicting inferior prognosis in DLBCL. Frequent CNVs of PI3K/AKT subunits may play an important role in the tumorigenesis of DLBCL. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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29. Role of MUC20 overexpression as a predictor of recurrence and poor outcome in colorectal cancer.
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Xiuying Xiao, Lisha Wang, Ping Wei, Yayun Chi, Dali Li, Qifeng Wang, Shujuan Ni, Cong Tan, Weiqi Sheng, Menghong Sun, Xiaoyan Zhou, and Xiang Du
- Subjects
COLON cancer patients ,MICE genetics ,GENE expression ,GENETIC regulation ,DISEASE relapse ,IMMUNOHISTOCHEMISTRY ,KAPLAN-Meier estimator - Abstract
Background: Colorectal cancer (CRC) remains one of the most common cancers worldwide. We observed that MUC20 was significantly up-regulated in CRC patients with poor prognosis based on the microarray analysis. However, little is known about the role of MUC20 in CRC. Methods: Microarray experiments were performed on the Affymetrix U133 plus 2.0 GeneChip Array. The protein and mRNA levels of MUC20 were examined by immunohistochemistry (IHC) and Real-Time quantitative PCR (RT-qPCR) in CRC tissues and adjacent noncancerous tissues (ANCT). ShRNA and overexpression plasmids were used to regulate MUC20 expression in CRC cell lines in vitro; wound healing, Transwell migration assays, and Western blotting were used to detect migration and invasion changes. Results: MUC20 was one of the up-regulated genes in CRC patients with poor prognosis by microarray. Using IHC and RT-qPCR, we showed that MUC20 expression was significantly higher in CRC tissues than in ANCT (P < 0.05). We further showed that MUC20 overexpression was correlated with recurrence and poor outcome (P < 0.05). The Kaplan-Meier survival curves indicated that disease-free survival (DFS) and overall survival (OS) were significantly worse in CRC patients with MUC20 overexpression. The Cox multivariate analysis revealed that MUC20 overexpression and TNM stage were independent prognostic factors. Elevated expression of MUC20 in cells promoted migration and invasion, whereas ShRNA-mediated knockdown inhibited these processes. In addition, Western blotting demonstrated that MUC20-induced invasion was associated with MMP-2, MMP-3, and E-cadherin. Conclusions: Cumulatively, MUC20 may serve as an important predictor of recurrence and poor outcome for CRC patients. MUC20 overexpression could enhance migration and invasion abilities of CRC cells. Translation of its roles into clinical practice will need further investigation and additional test validation. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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30. Prognostic potential of ERCC1 protein expression and clinicopathologic factors in stage III/N2 non-small cell lung cancer.
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Dong Yan, Ping Wei, Guangyu An, and Wenming Chen
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SMALL cell lung cancer , *IMMUNOLOGICAL adjuvants , *CISPLATIN , *ANTINEOPLASTIC agents , *TUMORS - Abstract
Background: Pathological stage III/N2 non-small cell lung cancer (NSCLC) is heterogeneous, and the optimal prognostic marker for survival remains unclear in Chinese patients. The aim of the present study was to assess the prognostic value of the clinicopathologic features and excision repair cross-complementing group-1 (ERCC1) in resected p-stage III/N2 NSCLC patients that received cisplatin-based adjuvant chemotherapy. Methods: Clinical data concerning 115 patients with histopathologically confirmed stage III/N2 NSCLC who underwent a complete resection were reviewed retrospectively. All patients received cisplatin-based adjuvant chemotherapy. The protein expression levels for ERCC1 were immunohistochemically examined in 115 patients. The relationship between the ERCC1 protein expression level and the clinical outcomes of the patients was then observed. Results: The 5-year survival rate and median survival time of patients with pathological stage III/N2 NSCLC after surgery and postoperative chemotherapy was 27.0% and 28.0 months, respectively. Survival of patients with ERCC1 negative tumors was significantly longer than those with ERCC1 positive tumors (p = 0.004). However, it was not entirely clear whether adjuvant chemotherapy with cisplatin-based agents was beneficial for ERCC1-negative patients with p-stage III/N2. A multivariate analysis of survival in patients with stage III/N2 NSCLC showed that surgical procedure (pneumonectomy vs. lobectomy; p = 0.001), number of involved lymph nodes (⩽5 vs. >5; p = 0.001) and ERCC1 protein expression (negative vs. positive; p = 0.012) were significant prognostic factors. In addition, the prognosis of patients with skip mediastinal lymph node metastasis showed a tendency for improved survival, but this was no significant (p = 0.432). Conclusions: Findings from this retrospective study suggested that the number of involved lymph nodes and the type of pulmonary resection are significant and independent prognosis factors in patients with p-stage III/N2 NSCLC. In addition, it was found that ERCC1 protein expression might play an important role in the prognosis of p-stage III/N2 NSCLC patients treated with cisplatin-based adjuvant chemotherapy. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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31. The effects of a histone deacetylase inhibitor on biological behavior of diffuse large B-cell lymphoma cell lines and insights into the underlying mechanisms.
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Ying Cai, Wenli Cui, Weixiang Chen, Ping Wei, Yayun Chi, Ping Zhang, Rui Bi, and Xiaoyan Zhou
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HISTONE deacetylase inhibitors ,EPIGENETICS ,CELL lines ,B cells ,FLOW cytometry - Abstract
Background: Epigenetic control using histone deacetylase (HDAC) inhibitors is a promising therapy for lymphomas. Insights into the anti-proliferative effects of HDAC inhibitors on diffuse large B-cell lymphoma (DLBCL) and further understanding of the underlying mechanisms, which remain unclear to date, are of great importance. Methods: Three DLBCL cell lines (DoHH2, LY1 and LY8) were used to define the potential epigenetic targets for Trichostatin A (TSA)-mediated anti-proliferative effects via CCK-8 assay. Cell cycle distribution and apoptosis were detected by flow cytometry. We further investigated the underlying molecular mechanisms by examining expression levels of relevant proteins using western blot analysis. Results: TSA treatment inhibited the growth of all three DLBCL cell lines and enhanced cell cycle arrest and apoptosis. Molecular analysis revealed upregulated acetylation of histone H3, α-tubulin and p53, and dephosphorylation of pAkt with altered expression of its main downstream effectors (p21, p27, cyclin D1 and Bcl-2). HDAC profiling revealed that all three cell lines had varying HDAC1-6 expression levels, with the highest expression of all six isoforms, in DoHH2 cells, which displayed the highest sensitivity to TSA. Conclusion: Our results demonstrated that the HDAC inhibitor TSA inhibited DLBCL cell growth, and that cell lines with higher expression of HDACs tended to be more sensitive to TSA. Our data also suggested that inhibition of pAkt and activation of p53 pathway are the main molecular events involved in inhibitory effects of TSA. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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32. TPX2 is a novel prognostic marker for the growth and metastasis of colon cancer.
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Ping Wei, Nu Zhang, Ye Xu, Xinxiang Li, Debing Shi, Yuwei Wang, Dawei Li, Sanjun Cai, Wei, Ping, Zhang, Nu, Xu, Ye, Li, Xinxiang, Shi, Debing, Wang, Yuwei, Li, Dawei, and Cai, Sanjun
- Subjects
- *
PROGNOSTIC tests , *COLON cancer , *METASTASIS , *MICROTUBULES , *GENE expression - Abstract
Background: We have previously demonstrated an aberrant overexpression of the microtubule-associated protein TPX2 in colon cancer using a genome-wide gene expression profiling analysis. Here, we aim to investigate its expression pattern, clinical significance, and biological function in colon cancer.Methods: TPX2 expression was analyzed in human colon cancer cell lines and tumor samples. The effect of TPX2 on cell proliferation, tumorigenesis, and metastasis was examined in vitro and in vivo.Results: TPX2 was overexpressed in 129 of the 203 (60.8%) colon cancer metastatic lesions, with the expression being significantly higher than that in primary cancerous tissue and normal colon mucosa. Overexpression of TPX2 was significantly associated with clinical staging, vessel invasion, and metastasis. In survival analyses, patients with TPX2 overexpression had worse overall survival and metastasis free survival, suggesting that deregulation of TPX2 may contribute to the metastasis of colon cancer. Consistent with this, suppression of TPX2 expression inhibited proliferation and tumorigenicity of colon cancer cells both in vitro and in vivo. Strikingly, we found that TPX2 knockdown significantly attenuated the migration and invasion ability of colon cancer cells, which was further shown to be mechanistically associated with AKT-mediated MMP2 activity.Conclusions: These findings suggest that TPX2 plays an important role in promoting tumorigenesis and metastasis of human colon cancer, and may represent a novel prognostic biomarker and therapeutic target for the disease. [ABSTRACT FROM AUTHOR]- Published
- 2013
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33. BAIAP2 exhibits association to childhood ADHD especially predominantly inattentive subtype in Chinese han subjects.
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Lu Liu, Li Sun, Ze-Hua Li, Hai-Mei Li, Li-Ping Wei, Yu-Feng Wang, and Qiu-Jin Qian
- Subjects
ATTENTION-deficit hyperactivity disorder ,ELECTROPHYSIOLOGY ,CEREBRAL hemispheres ,SINGLE nucleotide polymorphisms ,SCHIZOPHRENIA ,AUTISM - Abstract
Background Attention-deficit/hyperactivity disorder (ADHD) is a common chronic neurodevelopmental disorder with a high heritability. Much evidence of hemisphere asymmetry has been found for ADHD probands from behavioral level, electrophysiological level and brain morphology. One previous research has reported possible association between BAIAP2, which is asymmetrically expressed in the two cerebral hemispheres, with ADHD in European population. The present study aimed to investigate the association between BAIAP2 and ADHD in Chinese Han subjects. Methods A total of 1,397 ADHD trios comprised of one ADHD proband and their parents were included for family-based association tests. Independent 569 ADHD cases and 957 normal controls were included for case-control studies. Diagnosis was performed according to the DSM-IV criteria. Nine single nucleotide polymorphisms (SNPs) of BAIAP2 were chosen and performed genotyping for both family-based and case-control association studies. Results Transmission disequilibrium tests (TDTs) for family-based association studies showed significant association between the CA haplotype comprised by rs3934492 and rs9901648 with predominantly inattentive type (ADHD-I). For case-control study, chi-square tests provided evidence for the contribution of SNP rs4969239, rs3934492 and rs4969385 to ADHD and its two clinical subtypes, ADHD-I and ADHD-C. However, only the associations for ADHD and ADHD-I retained significant after corrections for multiplicity or logistic regression analyses adjusting the potential confounding effect of gender and age. Conclusions These above results indicated the possible involvement of BAIAP2 in the etiology of ADHD, especially ADHD-I. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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34. Comprehensive analysis of the overall codon usage patterns in equine infectious anemia virus.
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Xin Yin, Yuezhi Lin, Weigang Cai, Ping Wei, and Xiaojun Wang
- Subjects
EQUINE infectious anemia virus ,BLOOD diseases ,GENETIC mutation ,IMMUNE response ,GENOMICS - Abstract
Background Equine infectious anemia virus (EIAV) is an important animal model for understanding the relationship between viral persistence and the host immune response during lentiviral infections. Comparison and analysis of the codon usage model between EIAV and its hosts is important for the comprehension of viral evolution. In our study, the codon usage pattern of EIAV was analyzed from the available 29 full-length EIAV genomes through multivariate statistical methods. Finding Effective number of codons (ENC) suggests that the codon usage among EIAV strains is slightly biased. The ENC-plot analysis demonstrates that mutation pressure plays a substantial role in the codon usage pattern of EIAV, whereas other factors such as geographic distribution and host translation selection also take part in the process of EIAV evolution. Comparative analysis of codon adaptation index (CAI) values among EIAV and its hosts suggests that EIAV utilize the translational resources of horse more efficiently than that of donkey. Conclusion The codon usage bias in EIAV is slight and mutation pressure is the main factor that affects codon usage variation in EIAV. These results suggest that EIAV genomic biases are the result of the co-evolution of genome composition and the ability to evade the host's immune response. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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35. Abnormal expression of GADD45B in human colorectal carcinoma.
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Lisha Wang, Xiuying Xiao, Dali Li, Yayun Chi, Ping Wei, Yiqin Wang, Shujuan Ni, Cong Tan, Xiaoyan Zhou, and Xiang Du
- Subjects
DNA damage ,COLON cancer ,MESSENGER RNA ,IMMUNOHISTOCHEMISTRY ,WESTERN immunoblotting ,FLOW cytometry - Abstract
Background: GADD45B is a member of the growth arrest DNA damage-inducible gene family associated with cell growth control, apoptosis, and DNA damage repair response. The aim of this study is to detect the role of GADD45B in colorectal carcinoma (CRC); the area not studied in depth to date. Methods: The mRNA and protein levels of GADD45B were examined by Real-Time quantitative PCR (RT-qPCR) and immunohistochemistry (IHC) in CRC tissues and adjacent noncancerous tissues (ANCT). Over-expression plasmids and SiRNA were used to regulate GADD45B expression in CRC cell lines in vitro and flow cytometry and Western blotting were used to detect apoptotic changes. Results: The mRNA and protein levels of GADD45B were significantly higher in CRC tissues than those in ANCT (P<0.05). Up-regulation of GADD45B was also correlated with relapse and death of CRC patients (P<0.05). The Kaplan-Meier survival curves indicated that disease-free survival (DFS) was significantly worse in CRC patients who showed GADD45B overexpression. A Cox multivariate analysis revealed that GADD45B overexpression and TNM stage were significant factors affecting patients' survival. On the other hand, as a tumor suppressor gene, GADD45B amplified from normal colorectal tissues could induce apoptosis in CRC cell lines and may be associated with the p53-mediated apoptotic pathways. Conclusion: GADD45B, a tumor suppressor gene potentially through the p53-mediated apoptotic pathways, is paradoxically overexpressed in CRC and as such may play an unappreciated role in tumorigenesis. The exact mechanism of GADD45B inactivation and overexpression requires further investigation. GADD45B could be a potential therapeutic target for CRC treatment in future. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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36. Dextromethorphan Prevents Circulatory Failure in Rats with Endotoxemia.
- Author
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Chien-Chuan Wang, Yen-Mei Lee, Hsiao-Ping Wei, Chin-Chen Chu, and Mao-Hsiung Yen
- Subjects
ENDOTOXINS ,TUMOR necrosis factors ,NITRIC oxide ,ANIONS ,NEUTROPHILS - Abstract
Dextromethorphan (DM), an antitussive agent, has been claimed to have anti-inflammatory and immunomodulatory effects in vitro. Thus, the aim of this study was to evaluate the effects of DM on sepsis induced by intravenous (i.v.) administration of lipopolysaccharide (LPS) in anesthetized Wistar rats and by intraperitoneal administration in conscious ICR mice. Results demonstrated that pretreatment with DM (1, 5 and 10 mg/kg, i.v.) significantly attenuated the deleterious hemodynamic changes (e.g., hypotension and tachycardia) in rats treated with LPS. Meanwhile, DM (5 mg/kg) significantly inhibited the elevation of plasma tumor necrosis factor-α and interleukin-10 levels, as well as values of GOT and GPT (as an index of liver function), and BUN and creatinine (as an index of renal function) caused by LPS. The induction of inducible NO synthase and the overproduction of NO and superoxide anions by LPS were also reduced by DM. Moreover, infiltration of neutrophils into the lungs and liver of rats 6 h after treatment with LPS was also reduced by DM. In conclusion, the beneficial effects of DM on LPS-induced sepsis result from its anti-inflammatory and antioxidant effects. Thus, DM can possibly be used as a prophylactic agent for sepsis in the future. Copyright © 2004 National Science Council, ROC and S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2004
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37. The sole introduction of two single-point mutations establishes glycerol utilization in Saccharomyces cerevisiae CEN.PK derivatives.
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Ho PW, Swinnen S, Duitama J, and Nevoigt E
- Abstract
Background: Glycerol is an abundant by-product of biodiesel production and has several advantages as a substrate in biotechnological applications. Unfortunately, the popular production host Saccharomyces cerevisiae can barely metabolize glycerol by nature., Results: In this study, two evolved derivatives of the strain CEN.PK113-1A were created that were able to grow in synthetic glycerol medium (strains PW-1 and PW-2). Their growth performances on glycerol were compared with that of the previously published evolved CEN.PK113-7D derivative JL1. As JL1 showed a higher maximum specific growth rate on glycerol (0.164 h
-1 compared to 0.119 h-1 for PW-1 and 0.127 h-1 for PW-2), its genomic DNA was subjected to whole-genome resequencing. Two point mutations in the coding sequences of the genes UBR2 and GUT1 were identified to be crucial for growth in synthetic glycerol medium and subsequently verified by reverse engineering of the wild-type strain CEN.PK113-7D. The growth rate of the resulting reverse-engineered strain was 0.130 h-1 . Sanger sequencing of the GUT1 and UBR2 alleles of the above-mentioned evolved strains PW-1 and PW-2 also revealed one single-point mutation in these two genes, and both mutations were demonstrated to be also crucial and sufficient for obtaining a maximum specific growth rate on glycerol of ~0.120 h-1 ., Conclusions: The current work confirmed the importance of UBR2 and GUT1 as targets for establishing glycerol utilization in strains of the CEN.PK family. In addition, it shows that a growth rate on glycerol of 0.130 h-1 can be established in reverse-engineered CEN.PK strains by solely replacing a single amino acid in the coding sequences of both Ubr2 and Gut1.- Published
- 2017
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