7 results on '"Qingbo Li"'
Search Results
2. Mutual regulation between chicken telomerase reverse transcriptase and the Wnt/β-catenin signalling pathway inhibits apoptosis and promotes the replication of ALV-J in LMH cells
- Author
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Qingbo Li, Xiaoyan Chen, Jian Chen, Canxin Liang, Weisheng Cao, Zeng Jiang, Yu Li, Yong Xiang, Jinqun Li, and Yun Yu
- Subjects
Small interfering RNA ,Telomerase ,Carcinogenesis ,Veterinary medicine ,Caspase 3 ,Biology ,Virus Replication ,Cell Line ,Avian Proteins ,Cell Movement ,SF600-1100 ,Animals ,Telomerase reverse transcriptase ,Wnt Signaling Pathway ,Poultry Diseases ,avian leukosis virus subgroup J ,chicken telomerase reverse transcriptase ,General Veterinary ,Avian Leukosis Virus ,Cell growth ,Wnt signaling pathway ,apoptosis ,Wnt/β-catenin signalling pathway ,Cell cycle ,Hedgehog signaling pathway ,Cell biology ,cell proliferation ,Avian Leukosis ,Chickens ,Research Article - Abstract
This study aimed to explore the mutual regulation between chicken telomerase reverse transcriptase (chTERT) and the Wnt/β-catenin signalling pathway and its effects on cell growth and avian leukosis virus subgroup J (ALV-J) replication in LMH cells. First, LMH cells stably overexpressing the chTERT gene (LMH-chTERT cells) and corresponding control cells (LMH-NC cells) were successfully constructed with a lentiviral vector expression system. The results showed that chTERT upregulated the expression of β-catenin, Cyclin D1, TCF4 and c-Myc. chTERT expression level and telomerase activity were increased when cells were treated with LiCl. When the cells were treated with ICG001 or IWP-2, the activity of the Wnt/β-catenin signalling pathway was significantly inhibited, and chTERT expression and telomerase activity were also inhibited. However, when the β-catenin gene was knocked down by small interfering RNA (siRNA), the changes in chTERT expression and telomerase activity were consistent with those in cells treated with ICG001 or IWP-2. These results indicated that chTERT and the Wnt/β-catenin signalling pathway can be mutually regulated. Subsequently, we found that chTERT not only shortened the cell cycle to promote proliferation but also inhibited apoptosis by downregulating the expression of Caspase 3, Caspase 9 and BAX; upregulating BCL-2 and BCL-X expression; and promoting autophagy. Moreover, chTERT significantly enhanced the migration ability of LMH cells, upregulated the protein and mRNA expression of ALV-J and increased the virus titre. ALV-J replication promoted chTERT expression and telomerase activity. Supplementary Information The online version contains supplementary material available at 10.1186/s13567-021-00979-x.
- Published
- 2021
3. Herbal medicine foot bath for the treatment of diabetic peripheral neuropathy: protocol for a randomized, double-blind and controlled trial
- Author
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Xianyu Tang, Yuehong Zhou, Xiaotang Qiu, Haoyue Huang, Ling Zhao, Qingbo Li, Yuping Lin, Fang Dai, Fan Guanjie, Zhaohui Fang, Hua Wei, Yu Fu, Zhenjie Liu, Mei Wang, Guoqing Zheng, and Shi-Dong Wang
- Subjects
Adult ,Male ,medicine.medical_specialty ,China ,Time Factors ,Adolescent ,medicine.medical_treatment ,Medicine (miscellaneous) ,030209 endocrinology & metabolism ,law.invention ,03 medical and health sciences ,Study Protocol ,Young Adult ,0302 clinical medicine ,Randomized controlled trial ,Double-Blind Method ,law ,Diabetes mellitus ,Internal medicine ,medicine ,Humans ,Multicenter Studies as Topic ,Pharmacology (medical) ,030212 general & internal medicine ,Prospective Studies ,Risk factor ,Aged ,Randomized Controlled Trials as Topic ,lcsh:R5-920 ,business.industry ,Baths ,Middle Aged ,medicine.disease ,Diabetic foot ,Diabetic peripheral neuropathy ,Diabetic Foot ,Clinical trial ,Peripheral neuropathy ,Treatment Outcome ,Amputation ,External application ,Female ,Chinese herbal medicine ,Tangbi Waixi decoction ,Complication ,business ,lcsh:Medicine (General) ,Drugs, Chinese Herbal - Abstract
Background As a common complication of diabetes, the incidence of diabetic peripheral neuropathy (DPN) is 60–70% worldwide. DPN is a major risk factor for diabetic foot, which may lead to foot ulceration and even amputation. The treatment of DPN remains challenging. Our preliminary study demonstrated that the external application of Tangbi Waixi (TW) decoction to the lower extremities relieved clinical symptoms and improved nerve conduction velocity in DPN patients. The aim of this study was to verify the efficacy of TW among DPN patients and evaluate the herb mixture’s safety using rigorous methodological designs. Methods/design This study is a multicenter, double-blind, randomized controlled trial. A total of 640 DPN patients will be recruited and randomized to receive a foot bath with either the TW decoction or control drug. Participants will be assessed at baseline and 12 and 24 weeks after treatment. The primary outcome was the change of the Toronto Clinical Scoring System (TCSS). Secondary outcomes were nerve conduction velocity, blood glucose, blood lipids, serum inflammatory cytokines, and the European Quality of Life Five-Dimension Scale (EQ-5D) and TCM symptom scores. Discussion This multicenter, prospective, randomized clinical trial will provide valuable data regarding the efficacy and safety of foot bath treatment with TW decoction. Positive results would provide a novel treatment regimen for DPN patients. Trial registration Chinese Clinical Trial Registry, ChiCTR-IOR-16009331. Registered on 8 October 2016. Electronic supplementary material The online version of this article (10.1186/s13063-018-2856-4) contains supplementary material, which is available to authorized users.
- Published
- 2018
4. Minimally invasive versus open surgery for acute Achilles tendon rupture: a systematic review of overlapping meta-analyses.
- Author
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Qingbo Li, Chuanying Wang, Yanqing Huo, Zhiwei Jia, and Xiqian Wang
- Subjects
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CINAHL database , *COMPARATIVE studies , *MINIMALLY invasive procedures , *MEDICAL databases , *INFORMATION storage & retrieval systems , *MEDICAL information storage & retrieval systems , *MEDLINE , *META-analysis , *ONLINE information services , *OPERATIVE surgery , *TREATMENT effectiveness , *ACHILLES tendon rupture , *EVALUATION - Abstract
Background: A number of meta-analyses have been carried out to evaluate the effects of minimally invasive surgery (MIS) versus open surgery (OS) for acute Achilles tendon rupture. However, discordant findings were seen in these meta-analyses. The present study, performing a systematic review of overlapping meta-analyses regarding MIS versus OS of acute Achilles tendon rupture, aimed to assist decision-makers interpret and choose among conflicting meta-analyses, as well as to offer treatment recommendations based on current best evidence. Methods: The literature search was performed to identify systematic reviews comparing MIS with OS for Achilles tendon rupture. Meta-analyses only comprising randomized controlled trials (RCTs) were included. Two authors individually evaluated the quality of meta-analysis and extracted data. The Jadad decision algorithm was conducted to ascertain which meta-analysis offered the best evidence. Results: A total of four meta-analyses was included. All these meta-analyses comprised RCTs or quasi-RCTs and were determined as Level-II evidence. The scores of the Assessment of Multiple Systematic Reviews (AMSTAR) ranged from 7 to 10 (median 9.5). The Jadad algorithm indicated that the best meta-analysis should be chosen according to the search strategies and application of selection. A high-quality meta-analysis with more RCTs was chosen, which suggested that there was no statistically significant difference between MIS and OS regarding rerupture rate, tissue adhesion, sural nerve injury, deep infection, and deep vein thrombosis. However, MIS could decrease superficial infection rate, and had a better patient satisfaction for good to excellent outcomes in comparison to OS. Conclusions: Based on the best available evidence, MIS may be superior to OS for treating acute Achilles tendon rupture. However, due to some limitations, this should be cautiously interpreted, and further high-quality studies are needed. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
5. Significance analysis of microarray for relative quantitation of LC/MS data in proteomics
- Author
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Qingbo Li and Bryan P. Roxas
- Subjects
Spectrometry, Mass, Electrospray Ionization ,Proteome ,Quantitative proteomics ,Molecular Sequence Data ,Biology ,Proteomics ,lcsh:Computer applications to medicine. Medical informatics ,Biochemistry ,Peptide Mapping ,Sensitivity and Specificity ,Structural Biology ,Protein methods ,Liquid chromatography–mass spectrometry ,Sequence Analysis, Protein ,Amino Acid Sequence ,Molecular Biology ,lcsh:QH301-705.5 ,Oligonucleotide Array Sequence Analysis ,Chromatography ,Applied Mathematics ,Methodology Article ,Gene Expression Profiling ,Reproducibility of Results ,Replicate ,Molecular biology ,Fold change ,Computer Science Applications ,lcsh:Biology (General) ,lcsh:R858-859.7 ,DNA microarray ,Algorithms ,Chromatography, Liquid - Abstract
Background Although fold change is a commonly used criterion in quantitative proteomics for differentiating regulated proteins, it does not provide an estimation of false positive and false negative rates that is often desirable in a large-scale quantitative proteomic analysis. We explore the possibility of applying the Significance Analysis of Microarray (SAM) method (PNAS 98:5116-5121) to a differential proteomics problem of two samples with replicates. The quantitative proteomic analysis was carried out with nanoliquid chromatography/linear iron trap-Fourier transform mass spectrometry. The biological sample model included two Mycobacterium smegmatis unlabeled cell cultures grown at pH 5 and pH 7. The objective was to compare the protein relative abundance between the two unlabeled cell cultures, with an emphasis on significance analysis of protein differential expression using the SAM method. Results using the SAM method are compared with those obtained by fold change and the conventional t-test. Results We have applied the SAM method to solve the two-sample significance analysis problem in liquid chromatography/mass spectrometry (LC/MS) based quantitative proteomics. We grew the pH5 and pH7 unlabelled cell cultures in triplicate resulting in 6 biological replicates. Each biological replicate was mixed with a common 15N-labeled reference culture cells for normalization prior to SDS/PAGE fractionation and LC/MS analysis. For each biological replicate, one center SDS/PAGE gel fraction was selected for triplicate LC/MS analysis. There were 121 proteins quantified in at least 5 of the 6 biological replicates. Of these 121 proteins, 106 were significant in differential expression by the t-test (p < 0.05) based on peptide-level replicates, 54 were significant in differential expression by SAM with Δ = 0.68 cutoff and false positive rate at 5%, and 29 were significant in differential expression by the t-test (p < 0.05) based on protein-level replicates. The results indicate that SAM appears to overcome the false positives one encounters using the peptide-based t-test while allowing for identification of a greater number of differentially expressed proteins than the protein-based t-test. Conclusion We demonstrate that the SAM method can be adapted for effective significance analysis of proteomic data. It provides much richer information about the protein differential expression profiles and is particularly useful in the estimation of false discovery rates and miss rates.
- Published
- 2008
6. An assessment of false discovery rates and statistical significance in label-free quantitative proteomics with combined filters.
- Author
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Qingbo Li and Roxas, Bryan A. P.
- Subjects
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PROTEINS , *BIOMOLECULES , *ALGORITHMS , *MOLECULAR biology , *PROTEOMICS , *BIOINFORMATICS - Abstract
Background: Many studies have provided algorithms or methods to assess a statistical significance in quantitative proteomics when multiple replicates for a protein sample and a LC/MS analysis are available. But, confidence is still lacking in using datasets for a biological interpretation without protein sample replicates. Although a fold-change is a conventional threshold that can be used when there are no sample replicates, it does not provide an assessment of statistical significance such as a false discovery rate (FDR) which is an important indicator of the reliability to identify differentially expressed proteins. In this work, we investigate whether differentially expressed proteins can be detected with a statistical significance from a pair of unlabeled protein samples without replicates and with only duplicate LC/MS injections per sample. A FDR is used to gauge the statistical significance of the differentially expressed proteins. Results: We have experimented to operate on several parameters to control a FDR, including a fold-change, a statistical test, and a minimum number of permuted significant pairings. Although none of these parameters alone gives a satisfactory control of a FDR, we find that a combination of these parameters provides a very effective means to control a FDR without compromising the sensitivity. The results suggest that it is possible to perform a significance analysis without protein sample replicates. Only duplicate LC/MS injections per sample are needed. We illustrate that differentially expressed proteins can be detected with a FDR between 0 and 15% at a positive rate of 4-16%. The method is evaluated for its sensitivity and specificity by a ROC analysis, and is further validated with a [15N]-labeled internal-standard protein sample and additional unlabeled protein sample replicates. Conclusion: We demonstrate that a statistical significance can be inferred without protein sample replicates in label-free quantitative proteomics. The approach described in this study would be useful in many exploratory experiments where a sample amount or instrument time is limited. Naturally, this method is also suitable for proteomics experiments where multiple sample replicates are available. It is simple, and is complementary to other more sophisticated algorithms that are not designed for dealing with a small number of sample replicates. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
7. Significance analysis of microarray for relative quantitation of LC/MS data in proteomics.
- Author
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Roxas, Bryan A. P. and Qingbo Li
- Subjects
- *
DNA microarrays , *PROTEOMICS , *PROTEINS , *CELL culture , *QUANTITATIVE research - Abstract
Background: Although fold change is a commonly used criterion in quantitative proteomics for differentiating regulated proteins, it does not provide an estimation of false positive and false negative rates that is often desirable in a large-scale quantitative proteomic analysis. We explore the possibility of applying the Significance Analysis of Microarray (SAM) method (PNAS 98:5116-5121) to a differential proteomics problem of two samples with replicates. The quantitative proteomic analysis was carried out with nanoliquid chromatography/linear iron trap-Fourier transform mass spectrometry. The biological sample model included two Mycobacterium smegmatis unlabeled cell cultures grown at pH 5 and pH 7. The objective was to compare the protein relative abundance between the two unlabeled cell cultures, with an emphasis on significance analysis of protein differential expression using the SAM method. Results using the SAM method are compared with those obtained by fold change and the conventional t-test. Results: We have applied the SAM method to solve the two-sample significance analysis problem in liquid chromatography/mass spectrometry (LC/MS) based quantitative proteomics. We grew the pH5 and pH7 unlabelled cell cultures in triplicate resulting in 6 biological replicates. Each biological replicate was mixed with a common 15N-labeled reference culture cells for normalization prior to SDS/PAGE fractionation and LC/MS analysis. For each biological replicate, one center SDS/PAGE gel fraction was selected for triplicate LC/MS analysis. There were 121 proteins quantified in at least 5 of the 6 biological replicates. Of these 121 proteins, 106 were significant in differential expression by the t-test (p < 0.05) based on peptide-level replicates, 54 were significant in differential expression by SAM with Δ = 0.68 cutoff and false positive rate at 5%, and 29 were significant in differential expression by the t-test (p < 0.05) based on protein-level replicates. The results indicate that SAM appears to overcome the false positives one encounters using the peptide-based t-test while allowing for identification of a greater number of differentially expressed proteins than the protein-based t-test. Conclusion: We demonstrate that the SAM method can be adapted for effective significance analysis of proteomic data. It provides much richer information about the protein differential expression profiles and is particularly useful in the estimation of false discovery rates and miss rates. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
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