12 results on '"Shan Mei"'
Search Results
2. One repeated transplantation of allogeneic umbilical cord mesenchymal stromal cells in type 1 diabetes: an open parallel controlled clinical study
- Author
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Lu, Jing, Shen, Shan-mei, Ling, Qing, Wang, Bin, Li, Li-rong, Zhang, Wei, Qu, Duo-duo, Bi, Yan, and Zhu, Da-long
- Published
- 2021
- Full Text
- View/download PDF
3. Association analysis between the tag single nucleotide polymorphisms of DENND1A and the risk of polycystic ovary syndrome in Chinese Han women
- Author
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Zhu, Ya-nan, Zhang, Yi-ting, Liu, Qin, Shen, Shan-mei, Zou, Xiang, Cao, Yun-xia, Wang, Wen-jun, Yi, Long, Gao, Qian, Yang, Wei-dong, and Wang, Yong
- Published
- 2020
- Full Text
- View/download PDF
4. Effects of exenatide versus insulin glargine on body composition in overweight and obese T2DM patients: a randomized controlled trial
- Author
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Yin, Ting-Ting, Bi, Yan, Li, Ping, Shen, Shan-Mei, Wang, Wei-Min, Jiang, Can, Gao, Cai-Xia, Wang, Yan, Gao, Li-Jun, Zhu, Da-Long, and Feng, Wen-Huan
- Published
- 2018
- Full Text
- View/download PDF
5. Genome organisation of the Acinetobacter lytic phage ZZ1 and comparison with other T4-like Acinetobacter phages.
- Author
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Jing Jin, Zhen-Jiang Li, Shu-Wei Wang, Shan-Mei Wang, Song-Jian Chen, De-Hai Huang, Gai Zhang, Ya-Hui Li, Xiao-Ting Wang, Jin Wang, and Guo-Qiang Zhao
- Abstract
Background: Phage ZZ1, which efficiently infects pathogenic Acinetobacter baumannii strains, is the fifth completely sequenced T4-like Acinetobacter phage to date. To gain a better understanding of the genetic characteristics of ZZ1, bioinformatics and comparative genomic analyses of the T4 phages were performed. Results: The 166,687-bp double-stranded DNA genome of ZZ1 has the lowest GC content (34.4%) of the sequenced T4-like Acinetobacter phages. A total of 256 protein-coding genes and 8 tRNA genes were predicted. Forty-three percent of the predicted ZZ1 proteins share up to 73% amino acid identity with T4 proteins, and the homologous genes generally retained the same order and transcriptional direction. Beyond the conserved structural and DNA replication modules, T4 and ZZ1 have diverged substantially by the acquisition and deletion of large blocks of unrelated genes, especially in the first halves of their genomes. In addition, ZZ1 and the four other T4-like Acinetobacter phage genomes (Acj9, Acj61, 133, and Ac42) share a well-organised and highly conserved core genome, particularly in the regions encoding DNA replication and virion structural proteins. Of the ZZ1 proteins, 70, 64, 61, and 56% share up to 86, 85, 81, and 83% amino acid identity with Acj9, Acj61, 133, and Ac42 proteins, respectively. ZZ1 has a different number and types of tRNAs than the other 4 Acinetobacter phages, although some of the ZZ1-encoded tRNAs share high sequence similarity with the tRNAs from these phages. Over half of ZZ1-encoded tRNAs (5 out of 8) are related to optimal codon usage for ZZ1 proteins. However, this correlation was not present in any of the other 4 Acinetobacter phages. Conclusions: The comparative genomic analysis of these phages provided some new insights into the evolution and diversity of Acinetobacter phages, which might elucidate the evolutionary origin and host-specific adaptation of these phages. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
6. Identification of novel key amino acids at the interface of the transmembrane domains of human BST-2 and HIV-1 Vpu.
- Author
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Xiaojing Pang, Siqi Hu, Jian Li, Fengwen Xu, Shan Mei, Jinming Zhou, Shan Cen, Qi Jin, and Fei Guo
- Subjects
MESENCHYMAL stem cells ,AMINO acids ,CELLULAR signal transduction ,HIV ,FLUORESCENCE resonance energy transfer ,MOLECULAR dynamics - Abstract
Background: BST-2 (bone marrow stromal cell antigen 2) is an interferon-inducible protein that inhibits virus release by tethering viral particles to the cell surface. This antiviral activity of BST-2 is antagonized by HIV-1 accessory protein Vpu. Vpu physically interacts with BST-2 through their mutual transmembrane (TM) domains. In this study, we utilized the BRET assay and molecular dynamics (MD) simulation method to further characterize the interaction of BST-2 and Vpu. Results: Amino acids I34, L37, P40 and L41 in the TM domain of BST-2, and L11, A18 and W22 in the TM domain of Vpu were identified to be critical for the interaction between BST-2 and Vpu. The residues P40 in the TM domain of BST-2 and L11 in the TM domain of Vpu were shown, for the first time, to be important for their interaction. Furthermore, triple-amino-acid substitutions, 14–16 (AII to VAA) and 26–28 (IIE to AAA) in Vpu TM, not the single-residue mutation, profoundly disrupted BST-2/Vpu interaction. The results of MD simulation revealed significant conformational changes of the BST-2/Vpu complex as a result of mutating P40 of BST-2 and L11, 14–16 (AII to VAA) and 26–28 (IIE to AAA) of Vpu. In addition, disrupting the interaction between BST-2 and Vpu rendered BST-2 resistant to Vpu antagonization. Conclusions: Through use of the BRET assay, we identified novel key residues P40 in the TM domain of BST-2 and L11 in the TM domain of Vpu that are important for their interaction. These results add new insights into the molecular mechanism behind BST-2 antagonization by HIV-1 Vpu. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
7. Rapid screening for Chlamydia trachomatis infection by detecting α-mannosidase activity in urogenital tract specimens.
- Author
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Ze-yu Wang, Guang-yu Fu, Shan-mei Wang, Dong-chun Qin, Zhong-quan Wang, and Jing Cui
- Subjects
CHLAMYDIA trachomatis ,MANNOSIDASES ,GENITOURINARY diseases ,LIGASES ,PATHOGENIC microorganisms ,ENZYMATIC analysis ,GENETIC markers - Abstract
Background: Chlamydia trachomatis may cause multiple different urogenital tract disorders, but current non-culture assays for rapid screening of C. trachomatis typically use immunochromatography-based methods. We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of a-mannosidase enzymatic activity in urogenital tract specimens. Method: To evaluate the performance of this method, a-mannosidase activities of C. trachomatis serotype D strain ? and 29 standard strains related to clinical urogenital pathogens were investigated. Furthermore, 553 urogenital tract specimens were used for clinical assays via cell culture method and ligase chain reaction method (LCR), adopting an expanded gold standard. Results: Only C. trachomatis was positive for a-mannosidase activity among different types of microbes tested in the research. When prostate fluid specimens, which have some interfering activity, were excluded, the sensitivity and specificity of the enzymatic method were 91.8% (78/85) and 98.3% (409/416), respectively. There were no significant differences (P > 0.05). Conclusions: These results showed that a-mannosidase activity could be utilised as a screening marker of C. trachomatis infection. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
8. Isolation and characterization of ZZ1, a novel lytic phage that infects Acinetobacter baumannii clinical isolates.
- Author
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Jing Jin, Zhen-Jiang Li, Shu-Wei Wang, Shan-Mei Wang, De-Hai Huang, Ya-Hui Li, Yun-Yun Ma, Jin Wang, Fang Liu, Xiang-Dong Chen, Guang-Xing Li, Xiao-Ting Wang, Zhong-Quan Wang, and Guo-Qiang Zhao
- Subjects
ANTI-infective agents ,ACINETOBACTER baumannii ,ALTERNATIVE medicine ,BACTERIOPHAGES ,DNA - Abstract
Background: Acinetobacter baumannii, a significant nosocomial pathogen, has evolved resistance to almost all conventional antimicrobial drugs. Bacteriophage therapy is a potential alternative treatment for multidrug-resistant bacterial infections. In this study, one lytic bacteriophage, ZZ1, which infects A. baumannii and has a broad host range, was selected for characterization. Results: Phage ZZ1 and 3 of its natural hosts, A. baumanni clinical isolates AB09V, AB0902, and AB0901, are described in this study. The 3 strains have different sensitivities to ZZ1, but they have the same sensitivity to antibiotics. They are resistant to almost all of the antibiotics tested, except for polymyxin. Several aspects of the life cycle of ZZ1 were investigated using the sensitive strain AB09V under optimal growth conditions. ZZ1 is highly infectious with a short latent period (9 min) and a large burst size (200 PFU/cell). It exhibited the most powerful antibacterial activity at temperatures ranging from 35°C to 39°C. Moreover, when ZZ1 alone was incubated at different pHs and different temperatures, the phage was stable over a wide pH range (4 to 9) and at extreme temperatures (between 50°C and 60°C). ZZ1 possesses a 100-nm icosahedral head containing double-stranded DNA with a total length of 166,682 bp and a 120-nm long contractile tail. Morphologically, it could be classified as a member of the Myoviridae family and the Caudovirales order. Bioinformatic analysis of the phage whole genome sequence further suggested that ZZ1 was more likely to be a new member of the Myoviridae phages. Most of the predicted ORFs of the phage were similar to the predicted ORFs from other Acinetobacter phages. Conclusion: The phage ZZ1 has a relatively broad lytic spectrum, high pH stability, strong heat resistance, and efficient antibacterial potential at body temperature. These characteristics greatly increase the utility of this phage as an antibacterial agent; thus, it should be further investigated. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
9. Increasing risk behaviour can outweigh the benefits of antiretroviral drug treatment on the HIV incidence among men-having-sex-with-men in Amsterdam.
- Author
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Shan Mei, Quax, Rick, van de Vijver, David, Yifan Zhu, and Sloot, P. M. A.
- Subjects
- *
MEN who have sex with men , *HIV , *ANTIRETROVIRAL agents - Abstract
Background: The transmission through contacts among MSM (men who have sex with men) is one of the dominating contributors to HIV prevalence in industrialized countries. In Amsterdam, the capital of the Netherlands, the MSM risk group has been traced for decades. This has motivated studies which provide detailed information about MSM's risk behavior statistically, psychologically and sociologically. Despite the era of potent antiretroviral therapy, the incidence of HIV among MSM increases. In the long term the contradictory effects of risk behavior and effective therapy are still poorly understood. Methods: Using a previously presented Complex Agent Network model, we describe steady and casual partnerships to predict the HIV spreading among MSM. Behavior-related parameters and values, inferred from studies on Amsterdam MSM, are fed into the model; we validate the model using historical yearly incidence data. Subsequently, we study scenarios to assess the contradictory effects of risk behavior and effective therapy, by varying corresponding values of parameters. Finally, we conduct quantitative analysis based on the resulting incidence data. Results: The simulated incidence reproduces the ACS historical incidence well and helps to predict the HIV epidemic among MSM in Amsterdam. Our results show that in the long run the positive influence of effective therapy can be outweighed by an increase in risk behavior of at least 30% for MSM. Conclusion: We recommend, based on the model predictions, that lowering risk behavior is the prominent control mechanism of HIV incidence even in the presence of effective therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
10. Rapid screening for Chlamydia trachomatis infection by detecting α-mannosidase activity in urogenital tract specimens.
- Author
-
Wang, Ze-Yu, Fu, Guang-Yu, Wang, Shan-Mei, Qin, Dong-Chun, Wang, Zhong-Quan, and Cui, Jing
- Abstract
Background: Chlamydia trachomatis may cause multiple different urogenital tract disorders, but current non-culture assays for rapid screening of C. trachomatis typically use immunochromatography-based methods. We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of α-mannosidase enzymatic activity in urogenital tract specimens.Method: To evaluate the performance of this method, α-mannosidase activities of C. trachomatis serotype D strain 、 and 29 standard strains related to clinical urogenital pathogens were investigated. Furthermore, 553 urogenital tract specimens were used for clinical assays via cell culture method and ligase chain reaction method (LCR), adopting an expanded gold standard.Results: Only C. trachomatis was positive for α-mannosidase activity among different types of microbes tested in the research. When prostate fluid specimens, which have some interfering activity, were excluded, the sensitivity and specificity of the enzymatic method were 91.8% (78/85) and 98.3% (409/416), respectively. There were no significant differences (P > 0.05).Conclusions: These results showed that α-mannosidase activity could be utilised as a screening marker of C. trachomatis infection. [ABSTRACT FROM AUTHOR]- Published
- 2013
- Full Text
- View/download PDF
11. Genome organisation of the Acinetobacter lytic phage ZZ1 and comparison with other T4-like Acinetobacter phages.
- Author
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Jin J, Li ZJ, Wang SW, Wang SM, Chen SJ, Huang DH, Zhang G, Li YH, Wang XT, Wang J, and Zhao GQ
- Subjects
- Base Composition, Codon genetics, DNA Transposable Elements genetics, Evolution, Molecular, Genomics, Molecular Sequence Annotation, Phylogeny, RNA, Transfer genetics, Acinetobacter virology, Bacteriophage T4 genetics, Bacteriophage T4 physiology, Genome, Viral genetics
- Abstract
Background: Phage ZZ1, which efficiently infects pathogenic Acinetobacter baumannii strains, is the fifth completely sequenced T4-like Acinetobacter phage to date. To gain a better understanding of the genetic characteristics of ZZ1, bioinformatics and comparative genomic analyses of the T4 phages were performed., Results: The 166,687-bp double-stranded DNA genome of ZZ1 has the lowest GC content (34.4%) of the sequenced T4-like Acinetobacter phages. A total of 256 protein-coding genes and 8 tRNA genes were predicted. Forty-three percent of the predicted ZZ1 proteins share up to 73% amino acid identity with T4 proteins, and the homologous genes generally retained the same order and transcriptional direction. Beyond the conserved structural and DNA replication modules, T4 and ZZ1 have diverged substantially by the acquisition and deletion of large blocks of unrelated genes, especially in the first halves of their genomes. In addition, ZZ1 and the four other T4-like Acinetobacter phage genomes (Acj9, Acj61, 133, and Ac42) share a well-organised and highly conserved core genome, particularly in the regions encoding DNA replication and virion structural proteins. Of the ZZ1 proteins, 70, 64, 61, and 56% share up to 86, 85, 81, and 83% amino acid identity with Acj9, Acj61, 133, and Ac42 proteins, respectively. ZZ1 has a different number and types of tRNAs than the other 4 Acinetobacter phages, although some of the ZZ1-encoded tRNAs share high sequence similarity with the tRNAs from these phages. Over half of ZZ1-encoded tRNAs (5 out of 8) are related to optimal codon usage for ZZ1 proteins. However, this correlation was not present in any of the other 4 Acinetobacter phages., Conclusions: The comparative genomic analysis of these phages provided some new insights into the evolution and diversity of Acinetobacter phages, which might elucidate the evolutionary origin and host-specific adaptation of these phages.
- Published
- 2014
- Full Text
- View/download PDF
12. Isolation and characterization of ZZ1, a novel lytic phage that infects Acinetobacter baumannii clinical isolates.
- Author
-
Jin J, Li ZJ, Wang SW, Wang SM, Huang DH, Li YH, Ma YY, Wang J, Liu F, Chen XD, Li GX, Wang XT, Wang ZQ, and Zhao GQ
- Subjects
- Acinetobacter Infections microbiology, Acinetobacter baumannii drug effects, Acinetobacter baumannii isolation & purification, Anti-Bacterial Agents pharmacology, Bacteriophages genetics, Bacteriophages ultrastructure, Caudovirales genetics, Caudovirales isolation & purification, Caudovirales physiology, Caudovirales ultrastructure, DNA, Viral chemistry, DNA, Viral genetics, Drug Resistance, Multiple, Bacterial, Gene Order, Genome, Viral, Host Specificity, Humans, Hydrogen-Ion Concentration, Microbial Viability drug effects, Microbial Viability radiation effects, Molecular Sequence Data, Myoviridae genetics, Myoviridae isolation & purification, Myoviridae physiology, Myoviridae ultrastructure, Open Reading Frames, Sequence Analysis, DNA, Temperature, Virion ultrastructure, Acinetobacter baumannii virology, Bacteriophages isolation & purification, Bacteriophages physiology
- Abstract
Background: Acinetobacter baumannii, a significant nosocomial pathogen, has evolved resistance to almost all conventional antimicrobial drugs. Bacteriophage therapy is a potential alternative treatment for multidrug-resistant bacterial infections. In this study, one lytic bacteriophage, ZZ1, which infects A. baumannii and has a broad host range, was selected for characterization., Results: Phage ZZ1 and 3 of its natural hosts, A. baumanni clinical isolates AB09V, AB0902, and AB0901, are described in this study. The 3 strains have different sensitivities to ZZ1, but they have the same sensitivity to antibiotics. They are resistant to almost all of the antibiotics tested, except for polymyxin. Several aspects of the life cycle of ZZ1 were investigated using the sensitive strain AB09V under optimal growth conditions. ZZ1 is highly infectious with a short latent period (9 min) and a large burst size (200 PFU/cell). It exhibited the most powerful antibacterial activity at temperatures ranging from 35°C to 39°C. Moreover, when ZZ1 alone was incubated at different pHs and different temperatures, the phage was stable over a wide pH range (4 to 9) and at extreme temperatures (between 50°C and 60°C). ZZ1 possesses a 100-nm icosahedral head containing double-stranded DNA with a total length of 166,682 bp and a 120-nm long contractile tail. Morphologically, it could be classified as a member of the Myoviridae family and the Caudovirales order. Bioinformatic analysis of the phage whole genome sequence further suggested that ZZ1 was more likely to be a new member of the Myoviridae phages. Most of the predicted ORFs of the phage were similar to the predicted ORFs from other Acinetobacter phages., Conclusion: The phage ZZ1 has a relatively broad lytic spectrum, high pH stability, strong heat resistance, and efficient antibacterial potential at body temperature. These characteristics greatly increase the utility of this phage as an antibacterial agent; thus, it should be further investigated.
- Published
- 2012
- Full Text
- View/download PDF
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