Search

Your search keyword '"Valentin Jossen"' showing total 1 results
Start Over Author "Valentin Jossen" Publisher biomed central
Sorry, I don't understand your search. ×
1 results on '"Valentin Jossen"'

1. Expansion of mesenchymal adipose-tissue derived stem cells in a stirred single-use bioreactor under low-serum conditions

Author

Frank Jüngerkes, Regine Eibl, Christian van den Bos, Dieter Eibl, Stephan C. Kaiser, Valentin Jossen, Carmen Schirmaier, and Silke Brill

Subjects
Expansion rate, Volume (thermodynamics), Chemistry, Mesenchymal stem cell, Poster Presentation, Adipose tissue, General Medicine, Stem cell, General Biochemistry, Genetics and Molecular Biology, Single-use bioreactor, Biomedical engineering
Abstract

Materials and methods Preliminary tests in disposable-spinners (100 mL culture volume, Corning) were used to determine two suitable media and MC-types for serum reduced expansions (< 10%) of human adipose tissue-derived stem cells (hADSCs; passage 2, Lonza). Using such optimized media-MC-combinations, hADSCs expanded 30 to 40fold, which compares well with expansion rates in planar culture. Based on computational fluid dynamics simulations and suspension analyses in spinners [1], optimal operating parameters were determined in a BIOSTAT UniVessel SU 2 L (2 L culture volume, Sartorius Stedim Biotech).

Published
2013

Catalog

Books, media, physical & digital resources
See catalog results