1. SDF-1/CXCR4 axis enhances the immunomodulation of human endometrial regenerative cells in alleviating experimental colitis
- Author
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Kui Ye, Xiang Li, Yiming Zhao, Xiangying Gu, Xiaoxi Xu, Yonghao Hu, Hongyue Li, Caigan Du, Hao Wang, Grace Wang, Baoren Zhang, Ganggang Shi, and Xu Lan
- Subjects
0301 basic medicine ,Male ,Benzylamines ,Medicine (miscellaneous) ,Cyclams ,CXCR4 ,T-Lymphocytes, Regulatory ,Endometrial regenerative cells ,Endometrium ,0302 clinical medicine ,Heterocyclic Compounds ,Stromal cell-derived factor 1 ,lcsh:QD415-436 ,C-X-C chemokine receptor type 4 ,Cells, Cultured ,education.field_of_study ,lcsh:R5-920 ,Mice, Inbred BALB C ,biology ,Chemistry ,Colitis ,Flow Cytometry ,030220 oncology & carcinogenesis ,Molecular Medicine ,Female ,Stem cell ,lcsh:Medicine (General) ,Stromal cell-derived factor-1 ,Receptors, CXCR4 ,Stromal cell ,Population ,Enzyme-Linked Immunosorbent Assay ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,lcsh:Biochemistry ,03 medical and health sciences ,In vivo ,medicine ,Animals ,Humans ,education ,Research ,Immunoregulation ,Cell Biology ,Dendritic cell ,medicine.disease ,Chemokine CXCL12 ,030104 developmental biology ,biology.protein ,Cancer research ,Spleen - Abstract
Endometrial regenerative cells (ERCs) are a new type of mesenchymal-like stromal cells, and their therapeutic potential has been tested in a variety of disease models. SDF-1/CXCR4 axis plays a chemotaxis role in stem/stromal cell migration. The aim of the present study was to investigate the role of SDF-1/CXCR4 axis in the immunomodulation of ERCs on the experimental colitis. The immunomodulation of ERCs in the presence or absence of pretreatment of SDF-1 or AMD3100 was examined in both in vitro cell culture system and dextran sulphate sodium-induced colitis in mice. The results showed that SDF-1 increased the expression of CXCR4 on the surface of ERCs. As compared with normal ERCs, the SDF-1-treated, CXCR4 high-expressing ERCs more significantly suppressed dendritic cell population as well as stimulated both type 2 macrophages and regulatory T cells in vitro and in vivo. Meanwhile, SDF-1-pretreated ERCs increased the generation of anti-inflammatory factors (e.g., IL-4, IL-10) and decreased the pro-inflammatory factors (e.g., IL-6, TNF-α). In addition, SDF-1-pretreated CM-Dil-labeled ERCs were found to engraft to injured colon. Our results may suggest that an SDF-1-induced high level of CXCR4 expression enhances the immunomodulation of ERCs in alleviating experimental colitis in mice.
- Published
- 2019