1. Effects of Shenlian extract on experimental atherosclerosis in ApoE-deficient mice based on ultrasound biomicroscopy.
- Author
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Yan Guo, Xu-Cen Liu, Ya-Jie Wang, Qi Li, Qing Yang, Xiao-Gang Weng, Ying Chen, Wei-Yan Cai, Xiao-Xi Kan, Xi Chen, He-Fei Huang, Xiao-Xin Zhu, and Yu-Jie Li
- Subjects
ANIMAL experimentation ,APOLIPOPROTEINS ,ATHEROSCLEROSIS ,COMPARATIVE studies ,MICE ,MICROSCOPY ,RESEARCH funding ,STATISTICS ,ULTRASONIC imaging ,PLANT extracts ,DATA analysis ,DATA analysis software ,ONE-way analysis of variance - Abstract
Background: This study directly and dynamically investigated the effects of SL extract (i.e., a combination of Radix Salviae miltiorrhizae and Andrographis paniculata extract) on plaque progression in vivo by high resolution ultrasound biomicroscopy (UBM). Methods: An atherosclerosis model was established by placing a perivascular collar on the right common carotid artery in apolipoprotein E-deficient (ApoE
-/- ) mice. Thickness, plaque area and local blood flow were observed by UBM, pathological changes were observed by histochemical staining, and lipid levels were measured by respective commercially available kits. Results: Compared with the model group, the SL extract groups showed reduced wall thickness of the aortic arch (GC: P = 0.001, P = 0.002, and P < 0.001; LC: P < 0.001, P < 0.001, and P < 0.001; BC: P = 0.027, P = 0.017, and P = 0.003; respectively), which presented with retarded plaque progression of the cartoid artery with concordantly increased blood flow (P = 0.002 and P < 0.001) as visualized in vivo by UBM. Histological analysis confirmed the reduction of carotid atherosclerosis. Conclusions: The SL extract inhibited the formation of atherosclerotic plaques in an ApoE-/- mice model by UBM analysis, and did so by effects that ameliorated local blood flow and improved blood lipid levels. [ABSTRACT FROM AUTHOR]- Published
- 2016
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