23 results on '"Xu, Xiangyang"'
Search Results
2. CRISPR-Cas9-mediated mutagenesis of the SlSRM1-like gene leads to abnormal leaf development in tomatoes
- Author
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Tang, Yao, Li, Huijia, Liu, Chunxin, He, Yuqing, Wang, Hexuan, Zhao, Tingting, Xu, Xiangyang, Li, Jingfu, Yang, Huanhuan, and Jiang, Jingbin
- Published
- 2022
- Full Text
- View/download PDF
3. cAMP activates calcium signalling via phospholipase C to regulate cellulase production in the filamentous fungus Trichoderma reesei
- Author
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Chen, Yumeng, Fan, Xingjia, Zhao, Xinqing, Shen, Yaling, Xu, Xiangyang, Wei, Liujing, Wang, Wei, and Wei, Dongzhi
- Published
- 2021
- Full Text
- View/download PDF
4. Genome-wide identification and functional analysis of the ERF2 gene family in response to disease resistance against Stemphylium lycopersici in tomato
- Author
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Yang, Huanhuan, Sun, Yaoguang, Wang, Hexuan, Zhao, Tingting, Xu, Xiangyang, Jiang, Jingbin, and Li, Jingfu
- Published
- 2021
- Full Text
- View/download PDF
5. Functional analysis of the SlERF01 gene in disease resistance to S. lycopersici
- Author
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Yang, Huanhuan, Shen, Fengyi, Wang, Hexuan, Zhao, Tingting, Zhang, He, Jiang, Jingbin, Xu, Xiangyang, and Li, Jingfu
- Published
- 2020
- Full Text
- View/download PDF
6. Transcriptomic profiling of Solanum peruvianum LA3858 revealed a Mi-3-mediated hypersensitive response to Meloidogyne incognita
- Author
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Du, Chong, Jiang, Jingbin, Zhang, He, Zhao, Tingting, Yang, Huanhuan, Zhang, Dongye, Zhao, Zhentong, Xu, Xiangyang, and Li, Jingfu
- Published
- 2020
- Full Text
- View/download PDF
7. Ankle joint pressure change in varus malalignment of the tibia
- Author
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Zhu, Yuan, Li, Xingchen, and Xu, Xiangyang
- Published
- 2020
- Full Text
- View/download PDF
8. Comparative transcriptome analysis reveals the response mechanism of Cf-16-mediated resistance to Cladosporium fulvum infection in tomato
- Author
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Zhang, Dongye, Bao, Yufang, Sun, Yaoguang, Yang, Huanhuan, Zhao, Tingting, Li, Huijia, Du, Chong, Jiang, Jingbin, Li, Jingfu, Xie, Libo, and Xu, Xiangyang
- Published
- 2020
- Full Text
- View/download PDF
9. Transcriptome profiling reveals the response process of tomato carrying Cf-19 and Cladosporium fulvum interaction
- Author
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Zhao, Tingting, Liu, Wenhong, Zhao, Zhentong, Yang, Huanhuan, Bao, Yufang, Zhang, Dongye, Wang, Ziyu, Jiang, Jingbin, Xu, Ying, Zhang, He, Li, Jingfu, Chen, Qingshan, and Xu, Xiangyang
- Published
- 2019
- Full Text
- View/download PDF
10. Molecular mapping of the Cf-10 gene by combining SNP/InDel-index and linkage analysis in tomato (Solanum lycopersicum)
- Author
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Liu, Guan, Zhao, Tingting, You, Xiaoqing, Jiang, Jingbin, Li, Jingfu, and Xu, Xiangyang
- Published
- 2019
- Full Text
- View/download PDF
11. Surgical management of chronic lateral ankle instability: a meta-analysis
- Author
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Cao, Yongxing, Hong, Yuan, Xu, Yang, Zhu, Yuan, and Xu, Xiangyang
- Published
- 2018
- Full Text
- View/download PDF
12. Natural variation in genes potentially involved in plant architecture and adaptation in switchgrass (Panicum virgatum L.)
- Author
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Bahri, Bochra A., Daverdin, Guillaume, Xu, Xiangyang, Cheng, Jan-Fang, Barry, Kerrie W., Brummer, E. Charles, and Devos, Katrien M.
- Published
- 2018
- Full Text
- View/download PDF
13. Surgical treatment for diffused-type giant cell tumor (pigmented villonodular synovitis) about the ankle joint.
- Author
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Xingchen Li, Yang Xu, Yuan Zhu, Xiangyang Xu, Li, Xingchen, Xu, Yang, Zhu, Yuan, and Xu, Xiangyang
- Subjects
GIANT cell tumors ,TUMOR surgery ,ANKLE ,SYNOVIAL membranes ,JOINT surgery ,TUMOR treatment - Abstract
Background: Diffused-type giant cell tumor(Dt-GCT) is a rare, aggressive disorder of the joint synovium, bursa and tendon sheaths. Osseous erosions and subchondral cysts may develop as the result of synovium infiltration in Dt-GCT. We present a retrospective study of a series of patients who are diagnosed with Dt-GCT about the ankle joint, there clinical outcome is evaluated in this study.Material and Method: Fifteen patients with radiologically and histologically confirmed Dt-GCT about the ankle joint were identified in our foot and ankle department. Patients were managed with open synovectomy for the tumor tissue and bone grafting for bony erosions. X-rays and MRI scans were used for evaluation of the tumor and bony erosions pre- and post-operatively. Pre- and post-operative ankle function was assessed using the American Orthopedic Foot and Ankle Society -Ankle and Hindfoot (AOFAS-AH) score and the Muscularskeletal Tumor Society (MSTS) score.Results: The mean follow-up duration was 37.4 months (range 25 to 50 months). There were 6 males and 9 females, with a mean age of 35 years old (range 18 to 65 years). All patients had talar erosion with the average size of 10.1*9.1*8.2 mm, distal tibia was affected in 5 patients with the average size of 6.2*5.6*5.8 mm. 7 patients had tendon involvement, 2 patients had recurrence and progression of ankle osteoarthritis. Both of them underwent ankle fusion. At the time of last follow-up, the mean AOFAS-AH score increased from 49 to 80 points (p < 0.05), the MSTS score increased from 12 to 22 points (p < 0.05).Conclusion: For Dt-GCT with bony erosions, open synovectomy combined with bone grafting seems to be a safe and effective operation for the salvage of ankle joint. Fusion is recommended for failed and severe cartilage destruction of the ankle joint. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
14. Osteochondral autograft transplantation with biplanar distal tibial osteotomy for patients with concomitant large osteochondral lesion of the talus and varus ankle malalignment.
- Author
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Xingchen Li, Yuan Zhu, Yang Xu, Bibo Wang, Jinhao Liu, Xiangyang Xu, Li, Xingchen, Zhu, Yuan, Xu, Yang, Wang, Bibo, Liu, Jinhao, and Xu, Xiangyang
- Subjects
ANKLEBONE injuries ,OSTEOTOMY ,TRANSPLANTATION of organs, tissues, etc. ,AUTOGRAFTS ,ANKLE surgery ,TIBIA surgery ,ANKLEBONE surgery ,ANKLE ,ANKLE injuries ,CLUBFOOT ,LONGITUDINAL method ,OSTEOCHONDROSIS ,POSTOPERATIVE care ,WEIGHT-bearing (Orthopedics) ,DISEASE complications - Abstract
Background: Osteochondral lesions of the talus (OLTs) are amongst the most common foot and ankle disorders. Varus ankle malalignment causes stress concentration on medial side of the joint, resulting in OLTs and osteoarthritis. For large symptomatic OLTs (>10 mm), Osteochondral autograft transplantation is usually recommended. This article highlights biplanar distal tibial osteotomy as an approach and management for patients with concomitant large OLTs and varus ankle malalignment.Methods: From January 2012 to July 2014, 13 patients (6 male and 7 female) underwent surgery in our faculty and their average age was 55.4 (ranging from 34 to 69) years old. Oblique medial malleolar osteotomy was performed to expose the talar lesion, followed by an osteochondral autograft transplantation and distal tibial opening-wedge osteotomy. Weight-bearing X-rays were conducted and used for the measurement of radiographic parameters such as the tibial articular surface (TAS) and tibial lateral surface (TLS) angles. Ankle function of the subjects was evaluated according to the American Orthopaedic Foot and Ankle Society-Ankle and Hindfoot score (AOFAS-AH) questionnaires and Visual Analog Scale (VAS).Results: 11 patients completed the follow-up over a mean period of 21.2 months. The average area of talar lesion was 135.9 mm2 while the average depth was 11.4 mm. The mean time for osseous union was 8.5 weeks. Donor site morbidity was not recorded in any of the cases. The mean AOFAS-AH and VAS improved from 53 to 90 points (p < 0.05) and 6.7 to 1.9 points (p < 0.05) respectively. The mean TAS angle improved from 83.1 to 90.3° (p < 0.05).Conclusions: Biplanar distal tibial osteotomy with the combination of osteochondral autograft transplantation could be used to address patients with concomitant large OLTs and varus ankle malalignment as this technique provides excellent visualization of the talar defect, favorable biomechanical environment for the ankle joint with high rate of good and excellent results. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
15. Which level is responsible for gluteal pain in lumbar disc hernia?
- Author
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Guofang Fang, Jianhe Zhou, Yutan Liu, Hongxun Sang, Xiangyang Xu, Zihai Ding, Fang, Guofang, Zhou, Jianhe, Liu, Yutan, Sang, Hongxun, Xu, Xiangyang, and Ding, Zihai
- Subjects
BUTTOCK diseases ,PAIN ,BACKACHE ,INTERVERTEBRAL disk hernias ,LUMBAR vertebrae ,DISCECTOMY ,MAGNETIC resonance imaging ,INTERVERTEBRAL disk surgery ,BUTTOCKS ,ENDOSCOPY ,INTERVERTEBRAL disk ,INTERVERTEBRAL disk displacement ,RADICULOPATHY ,TREATMENT effectiveness ,RETROSPECTIVE studies ,LUMBAR pain ,DISEASE complications ,INNERVATION - Abstract
Background: There are many different reasons why patients could be experiencing pain in the gluteal area. Previous studies have shown an association between radicular low back pain (LBP) and gluteal pain (GP). Studies locating the specific level responsible for gluteal pain in lumbar disc hernias have rarely been reported.Methods: All patients with lumbar disc herniation (LDH) in the Kanghua hospital from 2010 to 2014 were recruited. All patients underwent a lumbar spine MRI to clarify their LDH diagnosis, and patients were allocated to a GP group and a non-GP group. To determine the cause and effect relationship between LDH and GP, all of the patients were subjected to percutaneous endoscopic lumbar discectomy (PELD).Results: A total of 286 cases were included according to the inclusive criteria, with 168 cases in the GP group and 118 cases in the non-GP group. Of these, in the GP group, 159 cases involved the L4/5 level and 9 cases involved the L5/S1 level, while in the non-GP group, 43 cases involved the L4/5 level and 48 cases involved the L5/S1 level. PELD was performed in both groups. Gluteal pain gradually disappeared after surgery in all of the patients. Gluteal pain recrudesced in a patient with recurrent disc herniation (L4/5).Conclusions: As a clinical finding, gluteal pain is related to low lumbar disc hernia. The L4/5 level is the main level responsible for gluteal pain in lumbar disc hernia. No patients with gluteal pain exhibited involvement at the L3/4 level. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
16. Reliability of measurements on lateral ankle radiographs.
- Author
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Changjun Guo, Yuan Zhu, Mu Hu, Lianfu Deng, Xiangyang Xu, Guo, Changjun, Zhu, Yuan, Hu, Mu, Deng, Lianfu, and Xu, Xiangyang
- Subjects
ANKLE injuries ,RADIOGRAPHIC processing ,JOINTS (Anatomy) ,ANKLE lateral ligament ,PATIENT compliance ,ANKLE ,COMPARATIVE studies ,DIGITAL image processing ,RESEARCH methodology ,MEDICAL cooperation ,RADIOGRAPHY ,RESEARCH ,RESEARCH evaluation ,TIBIA ,EVALUATION research ,RESEARCH bias ,WEIGHT-bearing (Orthopedics) - Abstract
Background: The aims of our study were to evaluate the validation of measurement of weight-bearing lateral radiographs. Two hypotheses were tested: the measurements on the lateral radiographs are reliable, and a theoretical limit could be identified when a surgeon can "eyeball" an incongruous ankle joint on lateral radiographs.Methods: To test the first hypothesis, 3 experienced ankle surgeons evaluated 50 normal weight-bearing lateral radiographs of patients. The measurements assessed were the tibial lateral surface angle (TLS), the distance from the center of the talar joint circle to the longitudinal axis of the tibia (x) and the displacement from the center of the talar articular joint circle to the center of the distal tibia articular joint circle (d). To test the second hypothesis, we used CAD software to create schematic diagrams on which lateral radiographs of the ankle joint were not parallel (d = 1, 2, 3, 4 mm). Five experienced ankle surgeons were asked to judge whether the ankle articular surfaces were parallel. Intraobserver reliability was determined using the intraclass correlation coefficients (ICCs) and interobserver agreement by the Kendall coefficient of concordance.Results: First, the intraobserver reliability was high (Cronbach's alpha >0.80) with regard to radiographic measurements according to the ICC. Significant interobserver disagreement was found (Kendall tauB, p < 0.01) using the Kendall concordance coefficient. Second, when the d-value was 4 mm, all the observers identified the incongruous ankle joint at two separate times.Conclusions: Consultation with experienced foot and ankle surgeons and precise definitions for lateral measurement assessments do not guarantee a high level of agreement. Surgeons can observe an incongruous ankle joint on lateral radiographs when the d-value is 4 mm. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
17. Natural variation in genes potentially involved in plant architecture and adaptation in switchgrass (<italic>Panicum virgatum</italic> L.).
- Author
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Bahri, Bochra A., Daverdin, Guillaume, Xu, Xiangyang, Cheng, Jan-Fang, Barry, Kerrie W., Brummer, E. Charles, and Devos, Katrien M.
- Subjects
GENES ,SWITCHGRASS ,PLANT genetics ,GENOMES ,BIOINFORMATICS - Abstract
Background: Advances in genomic technologies have expanded our ability to accurately and exhaustively detect natural genomic variants that can be applied in crop improvement and to increase our knowledge of plant evolution and adaptation. Switchgrass (
Panicum virgatum L.), an allotetraploid (2n = 4× = 36) perennial C4 grass (Poaceae family) native to North America and a feedstock crop for cellulosic biofuel production, has a large potential for genetic improvement due to its high genotypic and phenotypic variation. In this study, we analyzed single nucleotide polymorphism (SNP) variation in 372 switchgrass genotypes belonging to 36 accessions for 12 genes putatively involved in biomass production to investigate signatures of selection that could have led to ecotype differentiation and to population adaptation to geographic zones. Results: A total of 11,682 SNPs were mined from ~ 15 Gb of sequence data, out of which 251 SNPs were retained after filtering. Population structure analysis largely grouped upland accessions into one subpopulation and lowland accessions into two additional subpopulations. The most frequent SNPs were in homozygous state within accessions. Sixty percent of the exonic SNPs were non-synonymous and, of these, 45% led to non-conservative amino acid changes. The non-conservative SNPs were largely in linkage disequilibrium with one haplotype being predominantly present in upland accessions while the other haplotype was commonly present in lowland accessions. Tajima's test of neutrality indicated thatPHYB, a gene involved in photoperiod response, was under positive selection in the switchgrass population.PHYB carried a SNP leading to a non-conservative amino acid change in the PAS domain, a region that acts as a sensor for light and oxygen in signal transduction. Conclusions: Several non-conservative SNPs in genes potentially involved in plant architecture and adaptation have been identified and led to population structure and genetic differentiation of ecotypes in switchgrass. We suggest here thatPHYB is a key gene involved in switchgrass natural selection. Further analyses are needed to determine whether any of the non-conservative SNPs identified play a role in the differential adaptation of upland and lowland switchgrass. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
18. Mapping and screening of the tomato Stemphylium lycopersici resistance gene, Sm, based on bulked segregant analysis in combination with genome resequencing.
- Author
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Yang H, Zhao T, Jiang J, Wang S, Wang A, Li J, and Xu X
- Subjects
- Ascomycota genetics, Solanum lycopersicum microbiology, Plant Diseases microbiology, Plant Proteins metabolism, Sequence Analysis, DNA, Whole Genome Sequencing, Antibiosis genetics, Ascomycota physiology, Solanum lycopersicum genetics, Plant Diseases genetics, Plant Proteins genetics
- Abstract
Background: Tomato gray leaf spot disease caused by Stemphylium lycopersici (S. lycopersici) is considered one of the major diseases of cultivated tomatoes. The only S. lycopersici resistance gene, Sm, was derived from the wild tomato species S. pimpinellifolium. Sm has been identified as an effective source of gray leaf spot resistance in tomatoes and has been mapped to tomato chromosome 11. In this study, the first bulked segregant analysis (BSA) combined with genome resequencing for the mapping and screening of the Sm candidate gene was performed., Results: Based on the resequencing results, we identified 50,968 Diff-markers, most of which were distributed on chromosome 11. A total of 37 genes were located in the interval of 0.26-Mb. The gene loci of resistant and susceptible lines were sequenced successfully using PCR products. The relative expression levels of candidate genes in resistant and susceptible lines were confirmed via qRT-PCR, Solyc11g011870.1.1 and Solyc11g011880.1.1 were identified through qRT-PCR. A marker, D5, which was cosegregated with the resistant locus, was identified according to the mutation of the Solyc11g011880.1.1 trait in the resistant line., Conclusions: The Sm gene was mapped to the short arm of chromosome 11. The candidate genes Solyc11g011870.1.1 and Solyc11g011880.1.1 displayed expression patterns related to the resistance response. This study will be valuable for Sm cloning and Sm gene breeding in tomato.
- Published
- 2017
- Full Text
- View/download PDF
19. Surgical treatment for diffused-type giant cell tumor (pigmented villonodular synovitis) about the ankle joint.
- Author
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Li X, Xu Y, Zhu Y, and Xu X
- Subjects
- Adolescent, Adult, Aged, Female, Humans, Male, Middle Aged, Retrospective Studies, Young Adult, Ankle Joint surgery, Bone Transplantation methods, Joint Diseases surgery, Synovectomy methods, Synovitis, Pigmented Villonodular surgery
- Abstract
Background: Diffused-type giant cell tumor(Dt-GCT) is a rare, aggressive disorder of the joint synovium, bursa and tendon sheaths. Osseous erosions and subchondral cysts may develop as the result of synovium infiltration in Dt-GCT. We present a retrospective study of a series of patients who are diagnosed with Dt-GCT about the ankle joint, there clinical outcome is evaluated in this study., Material and Method: Fifteen patients with radiologically and histologically confirmed Dt-GCT about the ankle joint were identified in our foot and ankle department. Patients were managed with open synovectomy for the tumor tissue and bone grafting for bony erosions. X-rays and MRI scans were used for evaluation of the tumor and bony erosions pre- and post-operatively. Pre- and post-operative ankle function was assessed using the American Orthopedic Foot and Ankle Society -Ankle and Hindfoot (AOFAS-AH) score and the Muscularskeletal Tumor Society (MSTS) score., Results: The mean follow-up duration was 37.4 months (range 25 to 50 months). There were 6 males and 9 females, with a mean age of 35 years old (range 18 to 65 years). All patients had talar erosion with the average size of 10.1*9.1*8.2 mm, distal tibia was affected in 5 patients with the average size of 6.2*5.6*5.8 mm. 7 patients had tendon involvement, 2 patients had recurrence and progression of ankle osteoarthritis. Both of them underwent ankle fusion. At the time of last follow-up, the mean AOFAS-AH score increased from 49 to 80 points (p < 0.05), the MSTS score increased from 12 to 22 points (p < 0.05)., Conclusion: For Dt-GCT with bony erosions, open synovectomy combined with bone grafting seems to be a safe and effective operation for the salvage of ankle joint. Fusion is recommended for failed and severe cartilage destruction of the ankle joint.
- Published
- 2017
- Full Text
- View/download PDF
20. Osteochondral autograft transplantation with biplanar distal tibial osteotomy for patients with concomitant large osteochondral lesion of the talus and varus ankle malalignment.
- Author
-
Li X, Zhu Y, Xu Y, Wang B, Liu J, and Xu X
- Subjects
- Adult, Ankle Injuries complications, Ankle Joint physiopathology, Female, Follow-Up Studies, Humans, Male, Middle Aged, Postoperative Care, Transplantation, Autologous, Weight-Bearing, Young Adult, Ankle Joint surgery, Clubfoot surgery, Osteochondritis surgery, Osteotomy methods, Talus surgery, Tibia surgery
- Abstract
Background: Osteochondral lesions of the talus (OLTs) are amongst the most common foot and ankle disorders. Varus ankle malalignment causes stress concentration on medial side of the joint, resulting in OLTs and osteoarthritis. For large symptomatic OLTs (>10 mm), Osteochondral autograft transplantation is usually recommended. This article highlights biplanar distal tibial osteotomy as an approach and management for patients with concomitant large OLTs and varus ankle malalignment., Methods: From January 2012 to July 2014, 13 patients (6 male and 7 female) underwent surgery in our faculty and their average age was 55.4 (ranging from 34 to 69) years old. Oblique medial malleolar osteotomy was performed to expose the talar lesion, followed by an osteochondral autograft transplantation and distal tibial opening-wedge osteotomy. Weight-bearing X-rays were conducted and used for the measurement of radiographic parameters such as the tibial articular surface (TAS) and tibial lateral surface (TLS) angles. Ankle function of the subjects was evaluated according to the American Orthopaedic Foot and Ankle Society-Ankle and Hindfoot score (AOFAS-AH) questionnaires and Visual Analog Scale (VAS)., Results: 11 patients completed the follow-up over a mean period of 21.2 months. The average area of talar lesion was 135.9 mm
2 while the average depth was 11.4 mm. The mean time for osseous union was 8.5 weeks. Donor site morbidity was not recorded in any of the cases. The mean AOFAS-AH and VAS improved from 53 to 90 points (p < 0.05) and 6.7 to 1.9 points (p < 0.05) respectively. The mean TAS angle improved from 83.1 to 90.3° (p < 0.05)., Conclusions: Biplanar distal tibial osteotomy with the combination of osteochondral autograft transplantation could be used to address patients with concomitant large OLTs and varus ankle malalignment as this technique provides excellent visualization of the talar defect, favorable biomechanical environment for the ankle joint with high rate of good and excellent results.- Published
- 2017
- Full Text
- View/download PDF
21. Which level is responsible for gluteal pain in lumbar disc hernia?
- Author
-
Fang G, Zhou J, Liu Y, Sang H, Xu X, and Ding Z
- Subjects
- Adult, Diskectomy, Percutaneous, Endoscopy, Female, Humans, Intervertebral Disc surgery, Intervertebral Disc Displacement diagnostic imaging, Intervertebral Disc Displacement surgery, Low Back Pain diagnostic imaging, Low Back Pain surgery, Lumbar Vertebrae diagnostic imaging, Magnetic Resonance Imaging, Male, Middle Aged, Radiculopathy diagnostic imaging, Radiculopathy surgery, Retrospective Studies, Treatment Outcome, Buttocks innervation, Intervertebral Disc pathology, Intervertebral Disc Displacement complications, Low Back Pain etiology, Lumbar Vertebrae pathology, Radiculopathy etiology
- Abstract
Background: There are many different reasons why patients could be experiencing pain in the gluteal area. Previous studies have shown an association between radicular low back pain (LBP) and gluteal pain (GP). Studies locating the specific level responsible for gluteal pain in lumbar disc hernias have rarely been reported., Methods: All patients with lumbar disc herniation (LDH) in the Kanghua hospital from 2010 to 2014 were recruited. All patients underwent a lumbar spine MRI to clarify their LDH diagnosis, and patients were allocated to a GP group and a non-GP group. To determine the cause and effect relationship between LDH and GP, all of the patients were subjected to percutaneous endoscopic lumbar discectomy (PELD)., Results: A total of 286 cases were included according to the inclusive criteria, with 168 cases in the GP group and 118 cases in the non-GP group. Of these, in the GP group, 159 cases involved the L4/5 level and 9 cases involved the L5/S1 level, while in the non-GP group, 43 cases involved the L4/5 level and 48 cases involved the L5/S1 level. PELD was performed in both groups. Gluteal pain gradually disappeared after surgery in all of the patients. Gluteal pain recrudesced in a patient with recurrent disc herniation (L4/5)., Conclusions: As a clinical finding, gluteal pain is related to low lumbar disc hernia. The L4/5 level is the main level responsible for gluteal pain in lumbar disc hernia. No patients with gluteal pain exhibited involvement at the L3/4 level.
- Published
- 2016
- Full Text
- View/download PDF
22. Reliability of measurements on lateral ankle radiographs.
- Author
-
Guo C, Zhu Y, Hu M, Deng L, and Xu X
- Subjects
- Adult, Aged, Female, Humans, Image Processing, Computer-Assisted, Male, Middle Aged, Observer Variation, Orthopedic Surgeons, Radiography, Reproducibility of Results, Weight-Bearing, Young Adult, Ankle diagnostic imaging, Ankle Joint diagnostic imaging, Tibia diagnostic imaging
- Abstract
Background: The aims of our study were to evaluate the validation of measurement of weight-bearing lateral radiographs. Two hypotheses were tested: the measurements on the lateral radiographs are reliable, and a theoretical limit could be identified when a surgeon can "eyeball" an incongruous ankle joint on lateral radiographs., Methods: To test the first hypothesis, 3 experienced ankle surgeons evaluated 50 normal weight-bearing lateral radiographs of patients. The measurements assessed were the tibial lateral surface angle (TLS), the distance from the center of the talar joint circle to the longitudinal axis of the tibia (x) and the displacement from the center of the talar articular joint circle to the center of the distal tibia articular joint circle (d). To test the second hypothesis, we used CAD software to create schematic diagrams on which lateral radiographs of the ankle joint were not parallel (d = 1, 2, 3, 4 mm). Five experienced ankle surgeons were asked to judge whether the ankle articular surfaces were parallel. Intraobserver reliability was determined using the intraclass correlation coefficients (ICCs) and interobserver agreement by the Kendall coefficient of concordance., Results: First, the intraobserver reliability was high (Cronbach's alpha >0.80) with regard to radiographic measurements according to the ICC. Significant interobserver disagreement was found (Kendall tauB, p < 0.01) using the Kendall concordance coefficient. Second, when the d-value was 4 mm, all the observers identified the incongruous ankle joint at two separate times., Conclusions: Consultation with experienced foot and ankle surgeons and precise definitions for lateral measurement assessments do not guarantee a high level of agreement. Surgeons can observe an incongruous ankle joint on lateral radiographs when the d-value is 4 mm.
- Published
- 2016
- Full Text
- View/download PDF
23. Mapping and candidate gene screening of tomato Cladosporium fulvum-resistant gene Cf-19, based on high-throughput sequencing technology.
- Author
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Zhao T, Jiang J, Liu G, He S, Zhang H, Chen X, Li J, and Xu X
- Subjects
- Chromosome Mapping, Chromosomes, Plant, DNA, Plant, Genes, Plant, Genetic Markers, High-Throughput Nucleotide Sequencing, Solanum lycopersicum immunology, Solanum lycopersicum microbiology, Plant Diseases immunology, Plant Diseases microbiology, Plant Leaves, Real-Time Polymerase Chain Reaction, Cladosporium, Solanum lycopersicum genetics, Plant Diseases genetics
- Abstract
Background: Tomato leaf mold is a common disease in tomato cultivation. This disease is caused by Cladosporium fulvum, which has many physiological races and differentiates rapidly. Cf genes confer resistance to C. fulvum, and the C. fulvum-tomato pathosystem is a model for the study of gene-for-gene interactions. Plants carrying the Cf-19 gene show effective resistance to C. fulvum in the field, and can be used in breeding and resistance mechanism studies as new resistant materials. In this study, we used F2 bulk specific-locus amplified fragment sequencing (SLAF-seq) and parental resequencing methods to locate and characterize the Cf-19 gene., Results: A total of 4108 Diff_markers and three association regions were found in association analysis. A 2.14-Mb region containing seven Cf-type genes was identified in further analysis based on data from SLAF-seq and parental resequencing. Two candidate genes, Solyc01g006550.2.1 and Solyc01g005870.1.1, were screened out by quantitative real-time PCR (qRT-PCR) analysis. Sequence analysis showed that Solyc01g006550.2.1 (an allelic locus of Cf-0) in CGN18423 was a novel homologue of the Cladosporium resistance gene Cf-9 (Hcr9s) in the Cf-4/9 locus. The marker P7, which cosegregated with the resistant trait, was developed based on sequence mutation of the Solyc01g006550.2.1 locus in CGN18423., Conclusions: The Cf-19 gene was mapped to the short arm of chromosome 1. The candidate genes Solyc01g006550.2.1 and Solyc01g005870.1.1 showed related amino acid sequence structures and expression patterns. Solyc01g006550.2.1 had a close evolutionary relationship with the functional Hcr9 members Cf-4 and Cf-9, and was very different from non-functional members. The results from this study will facilitate the breeding of cultivars carrying the Cf-19 gene and provide a basis for further gene cloning, resistance gene evolution and plant resistance mechanism studies.
- Published
- 2016
- Full Text
- View/download PDF
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