47 results on '"Ya, Jing"'
Search Results
2. Axillary lymph node metastasis in pure mucinous carcinoma of breast: clinicopathologic and ultrasonographic features
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Li, Na, Li, Jia-Wei, Qian, Yu, Liu, Ya-Jing, Qi, Xiu-Zhu, Chen, Ya-Ling, Gao, Yi, and Chang, Cai
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- 2024
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3. Autophagy dysfunction contributes to NLRP1 inflammasome-linked depressive-like behaviors in mice
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Zhu, Ya-Jing, Huang, Jing, Chen, Ru, Zhang, Yu, He, Xin, Duan, Wen-Xin, Zou, Yuan-Lei, Sun, Meng-Mei, Sun, Hui-Li, Cheng, Si-Min, Wang, Hao-Chuan, Zhang, Hao, and Wu, Wen-Ning
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- 2024
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4. Inhibition of astroglial hemichannels ameliorates infrasonic noise induced short-term learning and memory impairment
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Zhang, Wei, Yin, Jue, Gao, Bei-Yao, Lu, Xi, Duan, Ya-Jing, Liu, Xu-Yan, Li, Ming-Zhen, and Jiang, Shan
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- 2023
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5. How do inpatients’ costs, length of stay, and quality of care vary across age groups after a new case-based payment reform in China? An interrupted time series analysis
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Chen, Ya-jing, Zhang, Xin-yu, Tang, Xue, Yan, Jia-qi, Qian, Meng-cen, and Ying, Xiao-hua
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- 2023
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6. Does information disclosure among public hospitals stimulate medical cost change efforts? A pilot study in Shanghai
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Chen, Ya-jing, Wang, Rui-Xin, Tang, Jin-yan, and Ying, Xiao-hua
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- 2023
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7. An entomopathogenic fungus exploits its host humoral antibacterial immunity to minimize bacterial competition in the hemolymph
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Wang, Jia-Lin, Sun, Jing, Song, Ya-Jing, Zheng, Huan-Huan, Wang, Gui-Jie, Luo, Wen-Xia, Li, Li, and Liu, Xu-Sheng
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- 2023
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8. Prognostic value of fibrinogen-to-albumin ratio combined with coronary calcification score in patients with suspected coronary artery disease
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Tian, Xin-Xin, Luo, Jun-Yi, Liu, Fen, Qiu, Ya-Jing, Luo, Fan, Zeng, Lu, Zhang, Zhuo-Ran, Yang, Yi-Ning, and Li, Xiao-Mei
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- 2023
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9. Data-driven study on resting-state functional magnetic resonance imaging during early abstinence of alcohol dependence in male patients and its predictive value for relapse
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Deng, Renhao, Yang, Xia, Meng, Ya-jing, Tao, Yu-jie, Wang, Hui-yao, Li, Xiao-jing, Wei, Wei, Yu, Hua, Wang, Qiang, Deng, Wei, Zhao, Lian-sheng, Ma, Xiao-hong, Li, Ming-li, Xu, Jia-jun, Li, Jing, Liu, Yan-song, Tang, Zhen, Du, Xiang-dong, Coid, Jeremy W., Greenshaw, Andrew J., Li, Tao, and Guo, Wan-jun
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- 2022
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10. LINC00665 knockdown confers sensitivity in irradiated non-small cell lung cancer cells through the miR-582-5p/UCHL3/AhR axis
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Xu, Li-Ming, Yuan, Ya-Jing, Yu, Hao, Wang, Shuai, and Wang, Ping
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- 2022
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11. The absence of AhR in CD4+ T cells in patients with acute graft-versus-host disease may be related to insufficient CTCF expression
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Zeng, Cong, Cheng, Ting-ting, Ma, Xia, Liu, Yi, Hua, Juan, Chen, Xu, Wang, Shi-yu, and Xu, Ya-jing
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- 2022
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12. Morphological changes of the root apex in anterior teeth with periapical periodontitis: an in-vivo study
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Zhang, Chen-chen, Liu, Ya-jing, Yang, Wei-dong, Zhang, Qian-nan, Zha, Ming-zhu, Wen, Shan-hui, and Wang, Qi
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- 2022
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13. The draft genome of the specialist flea beetle Altica viridicyanea (Coleoptera: Chrysomelidae)
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Xue, Huai-Jun, Niu, Yi-Wei, Segraves, Kari A., Nie, Rui-E, Hao, Ya-Jing, Zhang, Li-Li, Cheng, Xin-Chao, Zhang, Xue-Wen, Li, Wen-Zhu, Chen, Run-Sheng, and Yang, Xing-Ke
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- 2021
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14. Exosomes secreted by chemoresistant ovarian cancer cells promote angiogenesis
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Li, Zhang, Yan-qing, Wang, Xiao, Yang, Shi-yi, Liu, Meng-qin, Yuan, Shu, Xian, Dong-yong, Yang, Ya-jing, Zheng, and Yan-xiang, Cheng
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- 2021
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15. Lower regional grey matter in alcohol use disorders: evidence from a voxel-based meta-analysis
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Li, Lei, Yu, Hua, Liu, Yihao, Meng, Ya-jing, Li, Xiao-jing, Zhang, Chengcheng, Liang, Sugai, Li, Ming-li, Guo, Wanjun, QiangWang, Deng, Wei, Ma, Xiaohong, Coid, Jeremy, and Li, Tao
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- 2021
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16. Detection of Listeria monocytogenes in a patient with meningoencephalitis using next-generation sequencing: a case report
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Lan, Zi-Wei, Xiao, Min-Jia, Guan, Yuan-lin, Zhan, Ya-Jing, and Tang, Xiang-Qi
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- 2020
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17. NLRP1 inflammasome contributes to chronic stress-induced depressive-like behaviors in mice
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Song, Ao-Qi, Gao, Bo, Fan, Jun-Juan, Zhu, Ya-Jing, Zhou, Jun, Wang, Yu-Ling, Xu, Li-Zhong, and Wu, Wen-Ning
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- 2020
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18. Spinal cord NLRP1 inflammasome contributes to dry skin induced chronic itch in mice
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Fan, Jun-Juan, Gao, Bo, Song, Ao-Qi, Zhu, Ya-Jing, Zhou, Jun, Li, Wei-Zu, Yin, Yan-Yan, and Wu, Wen-Ning
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- 2020
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19. Transcriptional insights into the CD8+ T cell response in mono-HIV and HCV infection
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Li, Si-Yao, Zhang, Zi-Ning, Jiang, Yong‑Jun, Fu, Ya‑Jing, and Shang, Hong
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- 2020
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20. CD4+CD38+ central memory T cells contribute to HIV persistence in HIV-infected individuals on long-term ART
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Song, Cheng-Bo, Zhang, Le-Le, Wu, Xian, Fu, Ya-Jing, Jiang, Yong-Jun, Shang, Hong, and Zhang, Zi-Ning
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- 2020
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21. Reduced eIF3d accelerates HIV disease progression by attenuating CD8+ T cell function
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Pan, Ying, Zhang, Zi-Ning, Yin, Lin-Bo, Fu, Ya-Jing, Jiang, Yong-Jun, and Shang, Hong
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- 2019
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22. Exercise training modalities in patients with type 2 diabetes mellitus: a systematic review and network meta-analysis
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Pan, Bei, Ge, Long, Xun, Yang-qin, Chen, Ya-jing, Gao, Cai-yun, Han, Xue, Zuo, Li-qian, Shan, Hou-qian, Yang, Ke-hu, Ding, Guo-wu, and Tian, Jin-hui
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- 2018
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23. Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals
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Li, Cheng, Zhang, Hui-Yu, Liang, Yan, Xia, Wei, Zhu, Qian, Zhang, Duo, Huang, Zhen, Liang, Gui-Lin, Xue, Rui-Hong, Qi, Hang, He, Xiao-Qing, Yuan, Jiang-Jing, Tan, Ya-Jing, Huang, He-Feng, and Zhang, Jian
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- 2018
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24. Morphological changes of the root apex in anterior teeth with periapical periodontitis: an in-vivo study
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Shan-hui Wen, Ming-zhu Zha, Chen-chen Zhang, Wei-dong Yang, Ya-jing Liu, Qi Wang, and Qian-nan Zhang
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Periapical periodontitis ,business.industry ,Research ,Cone-beam computed tomography ,Dentistry ,RK1-715 ,medicine.disease ,Anterior teeth ,Root apex ,Root Canal Therapy ,stomatognathic system ,Tooth Apex ,In vivo ,medicine ,cardiovascular system ,Humans ,3D reconstruction ,business ,Root apex morphology ,General Dentistry ,Periapical Periodontitis - Abstract
Introduction The aim was to analyze the morphological changes of root apex in anterior teeth with periapical periodontitis. Methods 32 untreated anterior teeth with periapical periodontitis were enrolled, compared with the healthy contralateral teeth. Two-dimensional measurement of Cone-beam computed tomography was used to determine the location and measure diameter of the apical constriction according to Schell’s methods. An open-source software (3D Slicer) was used to reconstruct the teeth. The apical constriction form was analysis according to Schell’s topography. The distances of apical constriction to apical foramen and anatomical apex were measured respectively. Results The difference value between buccolingual and mesiodistal diameter was (0.06 ± 0.09) mm and (0.04 ± 0.04) mm in periapical periodontitis and controls (p Conclusions The anterior teeth with periapical periodontitis had shorter distances of apical constriction to anatomical apex and apical foramen, bigger disparities between the diameters of buccolingual and mesiodistal, and higher proportion of flaring apical constriction.
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- 2022
25. The absence of AhR in CD4+ T cells in patients with acute graft-versus-host disease may be related to insufficient CTCF expression.
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Zeng, Cong, Cheng, Ting-ting, Ma, Xia, Liu, Yi, Hua, Juan, Chen, Xu, Wang, Shi-yu, and Xu, Ya-jing
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- 2022
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26. Spinal cord NLRP1 inflammasome contributes to dry skin induced chronic itch in mice
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Bo Gao, Yan-Yan Yin, Jun Zhou, Wen-Ning Wu, Ao-Qi Song, Weizu Li, Jun-Juan Fan, and Ya-Jing Zhu
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Male ,Dry skin ,Inflammasomes ,Immunology ,Genetic Vectors ,TRPV1 ,Nod ,Ether ,lcsh:RC346-429 ,Proinflammatory cytokine ,Acetone ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Mice ,Psoriasis ,medicine ,NLRP1 inflammasome ,Animals ,lcsh:Neurology. Diseases of the nervous system ,Chronic itch ,Adaptor Proteins, Signal Transducing ,Skin ,Spinal cord ,integumentary system ,business.industry ,General Neuroscience ,Research ,Pruritus ,Inflammasome ,Scratching ,medicine.disease ,Mice, Inbred C57BL ,Neurology ,chemistry ,Chronic Disease ,Female ,Capsazepine ,business ,Apoptosis Regulatory Proteins ,Inflammasome complex ,medicine.drug - Abstract
Background Dry skin itch is one of the most common skin diseases and elderly people are believed to be particularly prone to it. The inflammasome has been suggested to play an important role in chronic inflammatory disorders including inflammatory skin diseases such as psoriasis. However, little is known about the role of NLRP1 inflammasome in dry skin-induced chronic itch. Methods Dry skin-induced chronic itch model was established by acetone-ether-water (AEW) treatment. Spontaneous scratching behavior was recorded by video monitoring. The expression of nucleotide oligomerization domain (NOD)-like receptor protein 1 (NLRP1) inflammasome complexes, transient receptor potential vanilloid type 1 (TRPV1), and the level of inflammatory cytokines were determined by western blot, quantitative real-time PCR, and enzyme-linked immunosorbent assay (ELISA) kits. Nlrp1a knockdown was performed by an adeno-associated virus (AAV) vector containing Nlrp1a-shRNA-eGFP infusion. H.E. staining was used to evaluate skin lesion. Results AEW treatment triggers spontaneous scratching and significantly increases the expression of NLRP1, ASC, and caspase-1 and the levels of IL-1β, IL-18, IL-6, and TNF-α in the spinal cord and the skin of mice. Spinal cord Nlrp1a knockdown prevents AEW-induced NLRP1 inflammasome assembly, TRPV1 channel activation, and spontaneous scratching behavior. Capsazepine, a specific antagonist of TRPV1, can also inhibit AEW-induced inflammatory response and scratching behavior. Furthermore, elderly mice and female mice exhibited more significant AEW-induced scratching behavior than young mice and male mice, respectively. Interestingly, AEW-induced increases in the expression of NLRP1 inflammasome complex and the levels of inflammatory cytokines were more remarkable in elderly mice and female mice than in young mice and male mice, respectively. Conclusions Spinal cord NLRP1 inflammasome-mediated inflammatory response contributes to dry skin-induced chronic itch by TRPV1 channel, and it is also involved in age and sex differences of chronic itch. Inhibition of NLRP1 inflammasome may offer a new therapy for dry skin itch.
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- 2020
27. Transcriptional insights into the CD8+ T cell response in mono-HIV and HCV infection
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Ya‑Jing Fu, Yong‑Jun Jiang, Si-Yao Li, Zining Zhang, and Hong Shang
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0301 basic medicine ,Microarray ,T cell ,lcsh:Medicine ,HIV Infections ,Biology ,CD8-Positive T-Lymphocytes ,CD8+ T cells ,General Biochemistry, Genetics and Molecular Biology ,Transcriptome ,03 medical and health sciences ,Resolvers ,0302 clinical medicine ,Long-term non-progressors ,microRNA ,medicine ,Cytotoxic T cell ,Humans ,Gene ,miRNA-143-3p ,Coinfection ,Research ,Gene Expression Profiling ,lcsh:R ,virus diseases ,HIV ,General Medicine ,Hepatitis C ,TLR2 ,Meta-analysis ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Immunology ,HCV ,CD8 - Abstract
Background Disease progression in the absence of therapy varies significantly in mono-HIV and HCV infected individuals. Virus-specific CD8+ T cells play an important role in restricting lentiviral replication and determining the rate of disease progression during HIV and HCV mono- and co-infection. Thus, understanding the similarities in the characteristics of CD8+ T cells in mono-HIV and HCV infection at the transcriptomic level contributes to the development of antiviral therapy. In this study, a meta-analysis of CD8+ T cell gene expression profiles derived from mono-HIV and HCV infected individuals at different stages of disease progression, was conducted to understand the common changes experienced by CD8+ T cells. Methods Five microarray datasets, reporting CD8+ T cell mRNA expression of the mono-HIV and HCV infected patients, were retrieved from Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) were identified via integrative meta-analysis of expression data (INMEX) program. Network analysis methods were used to assess protein–protein interaction (PPI) networks, Gene Ontology (GO) terms and pathway enrichment for DEGs. MirDIP and miRDB online prediction tools were used to predict potential microRNAs (miRNAs) targeting hub genes. Results First, we identified 625 and 154 DEGs in the CD8+ T cells originating from mono-HIV and HCV chronic progressor patients, respectively, compared to healthy individuals. Among them, interferon-stimulated genes (ISGs) including ISG15, IFIT3, ILI44L, CXCL8, FPR1 and TLR2, were upregulated after mono-HIV and HCV infection. Pathway enrichment analysis of DEGs showed that the “cytokine–cytokine receptor interaction” and “NF-kappa B” signaling pathways were upregulated after mono-HIV and HCV infection. In addition, we identified 92 and 50 DEGs in the CD8+ T cells of HIV non-progressor and HCV resolver patients, respectively, compared with corresponding chronic progressors. We observed attenuated mitosis and reduced ISG expression in HIV non-progressors and HCV resolvers compared with the corresponding chronic progressors. Finally, we identified miRNA-143-3p, predicted to target both IFIT3 in HIV and STAT5A in HCV infection. Conclusions We identified DEGs and transcriptional patterns in mono-HIV and HCV infected individuals at different stages of disease progression and identified miRNA-143-3p with potential to intervene disease progression, which provides a new strategy for developing targeted therapies.
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- 2020
28. Transcriptional insights into the CD8+ T cell response in mono-HIV and HCV infection.
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Li, Si-Yao, Zhang, Zi-Ning, Jiang, Yong‑Jun, Fu, Ya‑Jing, Shang, Hong, Jiang, Yong-Jun, and Fu, Ya-Jing
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HIV ,GENE expression profiling ,PROTEIN-protein interactions ,DISEASE progression ,GENE ontology ,HIV infections ,RESEARCH ,META-analysis ,RESEARCH methodology ,HEPATITIS C ,MEDICAL cooperation ,EVALUATION research ,COMPARATIVE studies ,MIXED infections ,RESEARCH funding ,T cells - Abstract
Background: Disease progression in the absence of therapy varies significantly in mono-HIV and HCV infected individuals. Virus-specific CD8+ T cells play an important role in restricting lentiviral replication and determining the rate of disease progression during HIV and HCV mono- and co-infection. Thus, understanding the similarities in the characteristics of CD8+ T cells in mono-HIV and HCV infection at the transcriptomic level contributes to the development of antiviral therapy. In this study, a meta-analysis of CD8+ T cell gene expression profiles derived from mono-HIV and HCV infected individuals at different stages of disease progression, was conducted to understand the common changes experienced by CD8+ T cells.Methods: Five microarray datasets, reporting CD8+ T cell mRNA expression of the mono-HIV and HCV infected patients, were retrieved from Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) were identified via integrative meta-analysis of expression data (INMEX) program. Network analysis methods were used to assess protein-protein interaction (PPI) networks, Gene Ontology (GO) terms and pathway enrichment for DEGs. MirDIP and miRDB online prediction tools were used to predict potential microRNAs (miRNAs) targeting hub genes.Results: First, we identified 625 and 154 DEGs in the CD8+ T cells originating from mono-HIV and HCV chronic progressor patients, respectively, compared to healthy individuals. Among them, interferon-stimulated genes (ISGs) including ISG15, IFIT3, ILI44L, CXCL8, FPR1 and TLR2, were upregulated after mono-HIV and HCV infection. Pathway enrichment analysis of DEGs showed that the "cytokine-cytokine receptor interaction" and "NF-kappa B" signaling pathways were upregulated after mono-HIV and HCV infection. In addition, we identified 92 and 50 DEGs in the CD8+ T cells of HIV non-progressor and HCV resolver patients, respectively, compared with corresponding chronic progressors. We observed attenuated mitosis and reduced ISG expression in HIV non-progressors and HCV resolvers compared with the corresponding chronic progressors. Finally, we identified miRNA-143-3p, predicted to target both IFIT3 in HIV and STAT5A in HCV infection.Conclusions: We identified DEGs and transcriptional patterns in mono-HIV and HCV infected individuals at different stages of disease progression and identified miRNA-143-3p with potential to intervene disease progression, which provides a new strategy for developing targeted therapies. [ABSTRACT FROM AUTHOR]- Published
- 2020
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29. Effects of dexmedetomidine on TNF-α and interleukin-2 in serum of rats with severe craniocerebral injury
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Pai Peng, Qing-Hui Wang, Wan-Wei Jiang, Li-Xin Yin, Ya-Jing Liao, and Min Xu
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0301 basic medicine ,Agonist ,Male ,medicine.medical_specialty ,Adrenergic receptor ,medicine.drug_class ,Il-2 ,Femoral vein ,Severity of Illness Index ,lcsh:RD78.3-87.3 ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Anesthesiology ,Sedative/hypnotic ,medicine ,Adrenergic alpha-2 Receptor Agonists ,Animals ,Craniocerebral Trauma ,Dexmedetomidine ,business.industry ,Tumor Necrosis Factor-alpha ,Perioperative ,Rats ,Severe craniocerebral injury ,030104 developmental biology ,Anesthesiology and Pain Medicine ,lcsh:Anesthesiology ,Anesthesia ,TNF-α ,Interleukin-2 ,Tumor necrosis factor alpha ,business ,030217 neurology & neurosurgery ,Biomarkers ,medicine.drug ,Research Article - Abstract
Background Dexmedetomidine is a highly selective adrenergic receptor agonist, which has a dose-dependent sedative hypnotic effect. Furthermore, it also has pharmacological properties, and the ability to inhibit sympathetic activity and improve cardiovascular stability during an operation. However, its protective effect on patients with severe craniocerebral injury in the perioperative period remains unclear. Method Eighty adult male SD rats were used and divided into two groups (n = 40, each group): dexmedetomidine injury group (experimental group), and sodium chloride injury group (control group). Models of severe craniocerebral injury were established in these two groups using the modified Feeney’s free-fall method. As soon as the establishment of models was succeed, rat in the experimental group received 1 μg of dexmedetomidine (0.1 ml), while each rat in the control group was given 0.1 ml of 0.9% sodium chloride. Blood was sampled from an incision at the femoral vein to detect TNF-α and IL-2 levels at 1, 12, 24,36,48 and 72 h after establishing the model in the two groups. Results After severe craniocerebral injury, TNF-α levels of rats were lower in every stage and at different degrees in the experimental group than in the control group (P
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- 2017
30. Cupping therapy versus acupuncture for pain-related conditions: a systematic review of randomized controlled trials and trial sequential analysis
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Bao-Yong Lai, Ya-Jing Zhang, Xiao-Ying Yang, Guo-Yang Yang, Jianping Liu, Xin-Lin Li, and Huijuan Cao
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medicine.medical_specialty ,Trial sequential analysis ,medicine.medical_treatment ,Review ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,Acupuncture ,medicine ,Cervical spondylosis ,VDP::Medisinske Fag: 700 ,030212 general & internal medicine ,Adverse effect ,Pharmacology ,Cupping therapy ,business.industry ,Absolute risk reduction ,lcsh:Other systems of medicine ,lcsh:RZ201-999 ,medicine.disease ,Confidence interval ,VDP::Medical disciplines: 700 ,Complementary and alternative medicine ,Relative risk ,Physical therapy ,Systematic review ,business ,030217 neurology & neurosurgery - Abstract
Source at: https://doi.org/10.1186/s13020-017-0142-0 Background: Both cupping therapy and acupuncture have been used in China for a long time, and their target indications are pain-related conditions. There is no systematic review comparing the effectiveness of these two therapies. Objectives: To compare the beneficial effectiveness and safety between cupping therapy and acupuncture for pain-related conditions to provide evidence for clinical practice. Methods: Protocol of this review was registered in PROSPERO (CRD42016050986). We conducted literature search from six electronic databases until 31st March 2017. We included randomized trials comparing cupping therapy with acupuncture on pain-related conditions. Methodological quality of the included studies was evaluated by risk of bias tool. Mean difference, risk ratio, risk difference and their 95% confidence interval were used to report the estimate effect of the pooled results through meta-analysis or the results from each individual study. Trial sequential analysis (TSA) was applied to adjust random errors and calculate the sample size. Results: Twenty-three randomized trials with 2845 participants were included covering 12 pain-related conditions. All included studies were of poor methodological quality. Three meta-analyses were conducted, which showed similar clinical beneficial effects of cupping therapy and acupuncture for the rate of symptom improvement in cervical spondylosis (RR 1.13, 95% CI 1.01 to 1.26; n = 646), lateral femoral cutaneous neuritis (RR 1.10, 95% CI 1.00 to 1.22; n = 102) and scapulohumeral periarthritis (RR 1.31, 95% CI 1.15 to 1.51; n = 208). Results from other outcomes (such as visual analogue and numerical rating scale) in each study also showed no statistical significant difference between these two therapies for all included pain-related conditions. The results of TSA for cervical spondylosis demonstrated that the current available data have not reached a powerful conclusion. No serious adverse events related to cupping therapy or acupuncture was found in included studies. Conclusion: Cupping therapy and acupuncture are potentially safe, and they have similar effectiveness in relieving pain. However, further rigorous studies investigating relevant pain-related conditions are warranted to establish comparative effectiveness analysis between these two therapies. Cost-effectiveness studies should be considered in the future studies to establish evidence for decision-making in clinical practice.
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- 2017
31. CD4+CD38+ central memory T cells contribute to HIV persistence in HIV-infected individuals on long-term ART.
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Song, Cheng-Bo, Zhang, Le-Le, Wu, Xian, Fu, Ya-Jing, Jiang, Yong-Jun, Shang, Hong, and Zhang, Zi-Ning
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HIV ,BLOOD cells ,HIV-positive persons ,PERSISTENCE ,FLOW cytometry ,T cells ,HIV infections ,RESEARCH ,MONONUCLEAR leukocytes ,RESEARCH methodology ,ANTIRETROVIRAL agents ,MEDICAL cooperation ,EVALUATION research ,COMPARATIVE studies ,IMMUNITY ,RESEARCH funding ,QUESTIONNAIRES - Abstract
Background: Despite the effective antiretroviral treatment (ART) of HIV-infected individuals, HIV persists in a small pool. Central memory CD4+ T cells (Tcm) make a major contribution to HIV persistence. We found that unlike HLA-DR, CD38 is highly expressed on the Tcm of HIV-infected subjects receiving ART for > 5 years. It has been reported that the half-life of total and episomal HIV DNA in the CD4+CD38+ T cell subset, exhibits lower decay rates at 12 weeks of ART. Whether CD38 contributes to HIV latency in HIV-infected individuals receiving long-term ART is yet to be addressed.Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood of HIV-infected subjects receiving suppressive ART. The immunophenotyping, proliferation and apoptosis of CD4+ T cell subpopulations were detected by flow cytometry, and the level of CD38 mRNA and total HIV DNA were measured using real-time PCR and digital droplet PCR, respectively. A negative binomial regression model was used to determine the correlation between CD4+CD38+ Tcm and total HIV DNA in CD4+ T cells.Results: CD38 was highly expressed on CD4+ Tcm cells from HIV infected individuals on long-term ART. Comparing with HLA-DR-Tcm and CD4+HLA-DR+ T cells, CD4+CD38+ Tcm cells displayed lower levels of activation (CD25 and CD69) and higher levels of CD127 expression. The proportion of CD38+ Tcm, but not CD38- Tcm cells can predict the total HIV DNA in the CD4+ T cells and the CD38+ Tcm subset harbored higher total HIV DNA copy numbers than the CD38- Tcm subset. After transfected with CD38 si-RNA in CD4+ T cells, the proliferation of CD4+ T cells was inhibited.Conclusion: The current date indicates that CD4+CD38+ Tcm cells contribute to HIV persistence in HIV-infected individuals on long-term ART. Our study provides a potential target to resolve HIV persistence. [ABSTRACT FROM AUTHOR]- Published
- 2020
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32. Transcriptomic meta-analysis identifies gene expression characteristics in various samples of HIV-infected patients with nonprogressive disease.
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Le-Le Zhang, Zi-Ning Zhang, Xian Wu, Yong-Jun Jiang, Ya-Jing Fu, Hong Shang, Zhang, Le-Le, Zhang, Zi-Ning, Wu, Xian, Jiang, Yong-Jun, Fu, Ya-Jing, and Shang, Hong
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GENE expression ,HIV-positive persons ,META-analysis ,DISEASE progression ,BIOMARKERS ,GENE ontology ,BIOCHEMISTRY ,COMPARATIVE studies ,GENES ,GENETIC research ,HIV infections ,PHENOMENOLOGY ,RESEARCH methodology ,MEDICAL cooperation ,METABOLISM ,RESEARCH ,T cells ,EVALUATION research ,GENE expression profiling - Abstract
Background: A small proportion of HIV-infected patients remain clinically and/or immunologically stable for years, including elite controllers (ECs) who have undetectable viremia (<50 copies/ml) and long-term nonprogressors (LTNPs) who maintain normal CD4+ T cell counts for prolonged periods (>10 years). However, the mechanism of nonprogression needs to be further resolved. In this study, a transcriptome meta-analysis was performed on nonprogressor and progressor microarray data to identify differential transcriptome pathways and potential biomarkers.Methods: Using the INMEX (integrative meta-analysis of expression data) program, we performed the meta-analysis to identify consistently differentially expressed genes (DEGs) in nonprogressors and further performed functional interpretation (gene ontology analysis and pathway analysis) of the DEGs identified in the meta-analysis. Five microarray datasets (81 cases and 98 controls in total), including whole blood, CD4+ and CD8+ T cells, were collected for meta-analysis.Results: We determined that nonprogressors have reduced expression of important interferon-stimulated genes (ISGs), CD38, lymphocyte activation gene 3 (LAG-3) in whole blood, CD4+ and CD8+ T cells. Gene ontology (GO) analysis showed a significant enrichment in DEGs that function in the type I interferon signaling pathway. Upregulated pathways, including the PI3K-Akt signaling pathway in whole blood, cytokine-cytokine receptor interaction in CD4+ T cells and the MAPK signaling pathway in CD8+ T cells, were identified in nonprogressors compared with progressors. In each metabolic functional category, the number of downregulated DEGs was more than the upregulated DEGs, and almost all genes were downregulated DEGs in the oxidative phosphorylation (OXPHOS) and tricarboxylic acid (TCA) cycle in the three types of samples.Conclusions: Our transcriptomic meta-analysis provides a comprehensive evaluation of the gene expression profiles in major blood types of nonprogressors, providing new insights in the understanding of HIV pathogenesis and developing strategies to delay HIV disease progression. [ABSTRACT FROM AUTHOR]- Published
- 2017
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33. Aberrant expression and DNA methylation of lipid metabolism genes in PCOS: a new insight into its pathogenesis.
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Pan, Jie-Xue, Tan, Ya-Jing, Wang, Fang-Fang, Hou, Ning-Ning, Xiang, Yu-Qian, Zhang, Jun-Yu, Liu, Ye, Qu, Fan, Meng, Qing, Xu, Jian, Sheng, Jian-Zhong, and Huang, He-Feng
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POLYCYSTIC ovary syndrome , *METABOLISM - Abstract
Background: Polycystic ovary syndrome (PCOS), whose etiology remains uncertain, is a highly heterogenous and genetically complex endocrine disorder. The aim of this study was to identify differentially expressed genes (DEGs) in granulosa cells (GCs) from PCOS patients and make epigenetic insights into the pathogenesis of PCOS. Results: Included in this study were 110 women with PCOS and 119 women with normal ovulatory cycles undergoing in vitro fertilization acting as the control group. RNA-seq identified 92 DEGs unique to PCOS GCs in comparison with the control group. Bioinformatic analysis indicated that synthesis of lipids and steroids was activated in PCOS GCs. 5-Methylcytosine analysis demonstrated that there was an approximate 25% reduction in global DNA methylation of GCs in PCOS women (4.44 ± 0.65%) compared with the controls (6.07 ± 0.72%; P < 0.05). Using MassArray EpiTYPER quantitative DNA methylation analysis, we also found hypomethylation of several gene promoters related to lipid and steroid synthesis, which might result in the aberrant expression of these genes. Conclusions: Our results suggest that hypomethylated genes related to the synthesis of lipid and steroid may dysregulate expression of these genes and promote synthesis of steroid hormones including androgen, which could partially explain mechanisms of hyperandrogenism in PCOS. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
34. Effects of dexmedetomidine on TNF-α and interleukin-2 in serum of rats with severe craniocerebral injury.
- Author
-
Wan-Wei Jiang, Qing-Hui Wang, Ya-Jing Liao, Pai Peng, Min Xu, and Li-Xin Yin
- Subjects
ANIMAL experimentation ,COMPARATIVE studies ,IMIDAZOLES ,INFLAMMATORY mediators ,INTERLEUKIN-2 ,RATS ,SALT ,SYMPATHETIC nervous system ,TUMOR necrosis factors ,HEAD injuries ,ADRENERGIC agonists ,PHARMACODYNAMICS - Abstract
Background: Dexmedetomidine is a highly selective adrenergic receptor agonist, which has a dose-dependent sedative hypnotic effect. Furthermore, it also has pharmacological properties, and the ability to inhibit sympathetic activity and improve cardiovascular stability during an operation. However, its protective effect on patients with severe craniocerebral injury in the perioperative period remains unclear. Method: Eighty adult male SD rats were used and divided into two groups (n = 40, each group): dexmedetomidine injury group (experimental group), and sodium chloride injury group (control group). Models of severe craniocerebral injury were established in these two groups using the modified Feeney's free-fall method. As soon as the establishment of models was succeed, rat in the experimental group received 1 µg of dexmedetomidine (0.1 ml), while each rat in the control group was given 0.1 ml of 0.9% sodium chloride. Blood was sampled from an incision at the femoral vein to detect TNF-α and IL-2 levels at 1,12, 24,36,48 and 72 h after establishing the model in the two groups. Results: After severe craniocerebral injury, TNF-α levels of rats were lower in every stage and at different degrees in the experimental group than in the control group (P < 0.05), while IL-2 levels were lower in the experimental group to different extents (P < 0.05). Conclusion: Dexmedetomidine protects the brain of rats with severe craniocerebral injury by reducing the release of inflammatory mediators. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
35. Cupping therapy versus acupuncture for pain-related conditions: a systematic review of randomized controlled trials and trial sequential analysis.
- Author
-
Ya-Jing Zhang, Hui-Juan Cao, Xin-Lin Li, Xiao-Ying Yang, Bao-Yong Lai, Guo-Yang Yang, and Jian-Ping Liu
- Subjects
- *
PAIN management , *ACUPUNCTURE , *ALTERNATIVE medicine , *COMPARATIVE studies , *SYSTEMATIC reviews , *RANDOMIZED controlled trials - Abstract
Background: Both cupping therapy and acupuncture have been used in China for a long time, and their target indications are pain-related conditions. There is no systematic review comparing the effectiveness of these two therapies. Objectives: To compare the beneficial effectiveness and safety between cupping therapy and acupuncture for pain- related conditions to provide evidence for clinical practice. Methods: Protocol of this review was registered in PROSPERO (CRD42016050986). We conducted literature search from six electronic databases until 31st March 2017. We included randomized trials comparing cupping therapy with acupuncture on pain-related conditions. Methodological quality of the included studies was evaluated by risk of bias tool. Mean difference, risk ratio, risk difference and their 95% confidence interval were used to report the estimate effect of the pooled results through meta-analysis or the results from each individual study. Trial sequential analysis (TSA) was applied to adjust random errors and calculate the sample size. Results: Twenty-three randomized trials with 2845 participants were included covering 12 pain-related conditions. All included studies were of poor methodological quality. Three meta-analyses were conducted, which showed similar clinical beneficial effects of cupping therapy and acupuncture for the rate of symptom improvement in cervical spondylosis (RR 1.13, 95% CI 1.01 to 1.26; n = 646), lateral femoral cutaneous neuritis (RR 1.10, 95% CI 1.00 to 1.22; n = 102) and scapulohumeral periarthritis (RR 1.31, 95% CI 1.15 to 1.51; n = 208). Results from other outcomes (such as visual analogue and numerical rating scale) in each study also showed no statistical significant difference between these two therapies for all included pain-related conditions. The results of TSA for cervical spondylosis demonstrated that the current available data have not reached a powerful conclusion. No serious adverse events related to cupping therapy or acupuncture was found in included studies. Conclusion: Cupping therapy and acupuncture are potentially safe, and they have similar effectiveness in relieving pain. However, further rigorous studies investigating relevant pain-related conditions are warranted to establish comparative effectiveness analysis between these two therapies. Cost-effectiveness studies should be considered in the future studies to establish evidence for decision-making in clinical practice. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
36. Characteristics of clinical studies of summer acupoint herbal patching: a bibliometric analysis.
- Author
-
Fen Zhou, Dan Yang, Jing-yu Lu, Yan-fu Li, Kai-yue Gao, Ya-jing Zhou, Ruo-xue Yang, Juan Cheng, Xiao-xiong Qi, Lily Lai, George Lewith, and Jian-ping Liu
- Abstract
Background: Summer acupoint herbal patching (SAHP) has been widely used in China for thousands of years. This bibliometric analysis aims to provide a comprehensive review of the characteristics of clinical studies on SAHP for any condition. Methods: We included clinical studies such as randomized clinical trials (RCTs), controlled clinical studies (CCTs), case series (CSs), case reports (CRs), and cross-sectional studies on SAHP for any condition. Six databases were searched from date of inception to March 2015. Bibliometric information and study details such as study type, characteristics of participants, details of the intervention and comparison, and outcome were extracted and analyzed. Results: A total of 937 clinical studies were identified and which were published between 1977 and 2015. This included 404 RCTs, 52 CCTs, 458 CSs, 19 CRs and 4 cross-sectional studies and involved 232,138 participants aged 2 to 90 years from two countries. Almost all studies were from China (936, 99.89 %). The five conditions most commonly treated by SAHP were asthma (401, 42.80 %), chronic bronchitis (146, 15.58 %), allergic rhinitis (117, 12.49 %), chronic obstructive pulmonary disease (73, 7.79 %), and recurrent respiratory tract infection (42, 4.48 %). Among 502 controlled studies, the majority compared SAHP alone with different controls (16 categories, 275 comparisons). The most commonly used controls were western medicine, placebo, traditional Chinese medicine, no treatment and non-pharmaceutical traditional Chinese therapies. Composite outcome measures were the most frequently reported outcome (512, 69.19 %). Conclusion: A substantial amount of research on SAHP has been published in China and which predominantly focuses on respiratory conditions. The findings from this study can be used to inform further research by highlighting areas of greatest impact for SAHP. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
37. Effect of reduced energy density of close-up diets on dry matter intake, lactation performance and energy balance in multiparous Holstein cows.
- Author
-
Wenming Huang, Yujia Tian, Ya Jing Wang, Aminamu Simayi, Amingguli Yasheng, Zhaohai Wu, Shengli Li, and Zhijun Cao
- Subjects
DRY matter in animal nutrition ,HOLSTEIN-Friesian cattle ,LACTATION ,BIOENERGETICS ,ENERGY density ,MILK yield - Abstract
Energy intake prepartum is critically important to health, milk performance, and profitability of dairy cows. The objective of this study was to determine the effect of reduced energy density of close-up diets on dry matter intake (DMI), lactation performance and energy balance (EB) in multiparous Holstein cows which were housed in a free-stall barn and fed for ad libitum intake. Thirty-nine dry cows were blocked and assigned randomly to three groups fed a high energy density diet [HD, n = 13; 6.8 MJ of net energy for lactation (NEL)/kg; 14.0% crude protein (CP) ], or a middle energy density diet (MD, n = 13; 6.2 MJ NEL/kg; 14.0% CP), or a low energy density diet (LD, n = 13; 5.4 MJ NEL/kg; 14.0% CP) from 21 day before expected day of calving. After parturition, all cows were fed the same lactation diet to 70 day in milk (DIM). The DMI and NEL intake prepartum were decreased by the reduced energy density diets (P < 0.05). The LD group consumed 1.3 kg/d (DM) more diet compared with HD group in the last 24 h before calving. The milk yield and the postpartum DMI were increased by the reduced energy density diet prepartum (P < 0.05). The changes in BCS and BW prepartum and postpartum were not affected by prepartum diets. HD group had higher milk fat content and lower lactose content compared with LD group during the first 3 weeks of lactation (P < 0.05). The energy consumption for HD, MD and LD groups were 149.8%, 126.2% and 101.1% of their calculated energy requirements prepartum (P < 0.05), and 72.7%, 73.1% and 75.2% during the first 4 weeks postpartum, respectively. In conclusion, the low energy density prepartum diet was effective in controlling NEL intake prepartum, and was beneficial in increasing DMI and milk yield, and alleviating negative EB postpartum. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
38. Prevalence of hyperuricemia and its related risk factors in healthy adults from Northern and Northeastern Chinese provinces.
- Author
-
Ling Qiu, Xin-qi Cheng, Jie Wu, Jun-ting Liu, Tao Xu, Hai-tao Ding, Yan-hong Liu, Zeng-mei Ge, Ya-jing Wang, Hui-juan Han, Jing Liu, and Guang-jin Zhu
- Subjects
HYPERURICEMIA ,DISEASE risk factors ,DISEASE prevalence ,INSULIN resistance ,HEALTH education ,MEDICAL care costs ,QUALITY of life - Abstract
Background: Hyperuricemia (HUA) is a potential risk factor for developing insulin resistance, hypertension, dyslipidemia and cardiovascular disease. Therefore, we studied the prevalence of HUA and associated risk factors in the population of two provinces in northern China. Methods: Based on the research of Chinese Physiological Constant and Health Conditions conducted in 2008-2010, we enrolled 29,639 subjects in a randomized, stratified study in four sampling areas in Heilongjiang Province and the Inner Mongolia Autonomous Region. We collected 13,140 serum samples to determine biochemical indicators including uric acid(UA), glucose, blood lipids, liver function, and renal function, and finally a representative sample of 8439 aged 18 years and older was determined. We also defined and stratified HUA, hypertension, diabetes, obesity and lipid abnormalities according to international guidelines. Results: There were significant differences in the UA levels between different genders and regions. The total prevalence of HUA is 13.7%. Men had a higher prevalence of HUA than women (21% vs. 7.9%; P < 0.0001). As age increased, HUA prevalence decreased in men but rose in women. The suburbs of big cities had the highest HUA prevalence (18.7%), and in high-prevalence areas the proportion of women with HUA also increased. A stepwise logistic regression model was used to filter out twelve HUA risk factors, including age, gender, residence, hypercholesterolemia, hypertriglyceridemia, impaired fasting glucose, hypertension, obesity, abdominal obesity, CKD, drinking and sleeping. After adjusting for these factors, the odds ratio of HUA was 1.92 times higher in men than in women. Compared with agricultural and pastoral areas, the odds ratio of having HUA was 2.14 for participants in the suburbs of big cities and 1.57 in the center of big cities. Conclusions: The prevalence of HUA is high in northern China. The differences in HUA prevalence by geographic region suggested that unbalanced economic development and health education, therefore HUA prevention measures should be strengthened to improve quality of life and reduce health care costs. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
39. Effect of Bizhongxiao decoction and its dismantled formulae on IL-1 and TNF levels in collagen-induced arthritis in rat synovial joints.
- Author
-
Ya-jing Guo, Jiang Chen, Xin-gui Xiong, Dan Wu, Hao Zhu, and Qing-hua Liang
- Subjects
ARTHRITIS ,COLLAGEN ,JOINTS (Anatomy) ,AUTOIMMUNE diseases ,CHINESE medicine - Abstract
Background: Rheumatoid arthritis (RA), a chronic autoimmune disease, affects sufferers in many different ways. Treatment of this chronic condition is particularly challenging. Traditional Chinese Medicine (TCM) provides alternatives. Bizhongxiao decoction (BZX) is a TCM complex, which has been used clinically for many years to treat RA. The purpose of this study is to compare the effects of BZX decoction and its dismantled formulae on IL-1 and TNF-1 levels in rats with RA, and to elucidate its mechanism of action. Methods: Ninety healthy normal female SD rats were randomly divided into six groups: normal (control), model, BZX decoction, and the three dismantled formulae (I: heat-clearing and detoxication, II: dissipating dampness, and III: blood circulation promotion). Apart from the normal (control) group, the rats in each group were injected subcutaneously with bovine type II collagen and complete Freund adjuvant to establish a collagen-induced arthritis model, so that inhibition of foot swelling in the rats by BZX decoction and its dismantled formulae could be observed. Immunohistochemistry was used to assess the levels of the inflammatory cytokines IL-1 and TNF in synovial joints at various time points. Results: Twenty-one days after the model was established, the levels of TNF and IL-1 were significantly higher in the model group, BZX decoction group and dismantled formula groups I, II and III than in the normal controls (P < 0.05). The levels of these cytokines were significantly higher in the model group than the BZX decoction or the three dismantled formula groups (P <0.01). At longer times, the TNF and IL-1 levels in model group rose gradually; those in the BZX decoction and dismantled formula groups were gradually reduced. The cytokine levels in the BZX decoction group were lower than in the three dismantled formula groups and continued to decline. Conclusions: BZX decoction and the three dismantled formulae examined down-regulated the inflammatory factors IL-1 and TNF in collagen-induced arthritis rat models, but BZX exerted the strongest effect. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
40. Prevalence of hyperuricemia and its related risk factors in healthy adults from Northern and Northeastern Chinese provinces.
- Author
-
Qiu, Ling, Cheng, Xin-Qi, Wu, Jie, Liu, Jun-Ting, Xu, Tao, Ding, Hai-Tao, Liu, Yan-Hong, Ge, Zeng-Mei, Wang, Ya-Jing, Han, Hui-Juan, Liu, Jing, and Zhu, Guang-Jin
- Abstract
Background: Hyperuricemia (HUA) is a potential risk factor for developing insulin resistance, hypertension, dyslipidemia and cardiovascular disease. Therefore, we studied the prevalence of HUA and associated risk factors in the population of two provinces in northern China.Methods: Based on the research of Chinese Physiological Constant and Health Conditions conducted in 2008-2010, we enrolled 29,639 subjects in a randomized, stratified study in four sampling areas in Heilongjiang Province and the Inner Mongolia Autonomous Region. We collected 13,140 serum samples to determine biochemical indicators including uric acid(UA), glucose, blood lipids, liver function, and renal function, and finally a representative sample of 8439 aged 18 years and older was determined. We also defined and stratified HUA, hypertension, diabetes, obesity and lipid abnormalities according to international guidelines.Results: There were significant differences in the UA levels between different genders and regions. The total prevalence of HUA is 13.7%. Men had a higher prevalence of HUA than women (21% vs. 7.9%; P < 0.0001). As age increased, HUA prevalence decreased in men but rose in women. The suburbs of big cities had the highest HUA prevalence (18.7%), and in high-prevalence areas the proportion of women with HUA also increased. A stepwise logistic regression model was used to filter out twelve HUA risk factors, including age, gender, residence, hypercholesterolemia, hypertriglyceridemia, impaired fasting glucose, hypertension, obesity, abdominal obesity, CKD, drinking and sleeping. After adjusting for these factors, the odds ratio of HUA was 1.92 times higher in men than in women. Compared with agricultural and pastoral areas, the odds ratio of having HUA was 2.14 for participants in the suburbs of big cities and 1.57 in the center of big cities.Conclusions: The prevalence of HUA is high in northern China. The differences in HUA prevalence by geographic region suggested that unbalanced economic development and health education, therefore HUA prevention measures should be strengthened to improve quality of life and reduce health care costs. [ABSTRACT FROM AUTHOR]- Published
- 2013
- Full Text
- View/download PDF
41. Effects of dexmedetomidine on TNF-α and interleukin-2 in serum of rats with severe craniocerebral injury.
- Author
-
Jiang WW, Wang QH, Liao YJ, Peng P, Xu M, and Yin LX
- Subjects
- Adrenergic alpha-2 Receptor Agonists pharmacology, Animals, Biomarkers blood, Dexmedetomidine pharmacology, Interleukin-2 antagonists & inhibitors, Male, Rats, Rats, Sprague-Dawley, Severity of Illness Index, Tumor Necrosis Factor-alpha antagonists & inhibitors, Adrenergic alpha-2 Receptor Agonists therapeutic use, Craniocerebral Trauma blood, Craniocerebral Trauma drug therapy, Dexmedetomidine therapeutic use, Interleukin-2 blood, Tumor Necrosis Factor-alpha blood
- Abstract
Background: Dexmedetomidine is a highly selective adrenergic receptor agonist, which has a dose-dependent sedative hypnotic effect. Furthermore, it also has pharmacological properties, and the ability to inhibit sympathetic activity and improve cardiovascular stability during an operation. However, its protective effect on patients with severe craniocerebral injury in the perioperative period remains unclear., Method: Eighty adult male SD rats were used and divided into two groups (n = 40, each group): dexmedetomidine injury group (experimental group), and sodium chloride injury group (control group). Models of severe craniocerebral injury were established in these two groups using the modified Feeney's free-fall method. As soon as the establishment of models was succeed, rat in the experimental group received 1 μg of dexmedetomidine (0.1 ml), while each rat in the control group was given 0.1 ml of 0.9% sodium chloride. Blood was sampled from an incision at the femoral vein to detect TNF-α and IL-2 levels at 1, 12, 24,36,48 and 72 h after establishing the model in the two groups., Results: After severe craniocerebral injury, TNF-α levels of rats were lower in every stage and at different degrees in the experimental group than in the control group (P < 0.05), while IL-2 levels were lower in the experimental group to different extents (P < 0.05)., Conclusion: Dexmedetomidine protects the brain of rats with severe craniocerebral injury by reducing the release of inflammatory mediators.
- Published
- 2017
- Full Text
- View/download PDF
42. Transcriptomic meta-analysis identifies gene expression characteristics in various samples of HIV-infected patients with nonprogressive disease.
- Author
-
Zhang LL, Zhang ZN, Wu X, Jiang YJ, Fu YJ, and Shang H
- Subjects
- CD4-Positive T-Lymphocytes metabolism, CD8-Positive T-Lymphocytes metabolism, Down-Regulation genetics, Gene Ontology, Humans, Metabolic Networks and Pathways genetics, Transcriptome genetics, Up-Regulation genetics, Disease Progression, Gene Expression Profiling, Gene Expression Regulation, HIV Infections genetics, HIV Infections pathology
- Abstract
Background: A small proportion of HIV-infected patients remain clinically and/or immunologically stable for years, including elite controllers (ECs) who have undetectable viremia (<50 copies/ml) and long-term nonprogressors (LTNPs) who maintain normal CD4
+ T cell counts for prolonged periods (>10 years). However, the mechanism of nonprogression needs to be further resolved. In this study, a transcriptome meta-analysis was performed on nonprogressor and progressor microarray data to identify differential transcriptome pathways and potential biomarkers., Methods: Using the INMEX (integrative meta-analysis of expression data) program, we performed the meta-analysis to identify consistently differentially expressed genes (DEGs) in nonprogressors and further performed functional interpretation (gene ontology analysis and pathway analysis) of the DEGs identified in the meta-analysis. Five microarray datasets (81 cases and 98 controls in total), including whole blood, CD4+ and CD8+ T cells, were collected for meta-analysis., Results: We determined that nonprogressors have reduced expression of important interferon-stimulated genes (ISGs), CD38, lymphocyte activation gene 3 (LAG-3) in whole blood, CD4+ and CD8+ T cells. Gene ontology (GO) analysis showed a significant enrichment in DEGs that function in the type I interferon signaling pathway. Upregulated pathways, including the PI3K-Akt signaling pathway in whole blood, cytokine-cytokine receptor interaction in CD4+ T cells and the MAPK signaling pathway in CD8+ T cells, were identified in nonprogressors compared with progressors. In each metabolic functional category, the number of downregulated DEGs was more than the upregulated DEGs, and almost all genes were downregulated DEGs in the oxidative phosphorylation (OXPHOS) and tricarboxylic acid (TCA) cycle in the three types of samples., Conclusions: Our transcriptomic meta-analysis provides a comprehensive evaluation of the gene expression profiles in major blood types of nonprogressors, providing new insights in the understanding of HIV pathogenesis and developing strategies to delay HIV disease progression.- Published
- 2017
- Full Text
- View/download PDF
43. Cupping therapy versus acupuncture for pain-related conditions: a systematic review of randomized controlled trials and trial sequential analysis.
- Author
-
Zhang YJ, Cao HJ, Li XL, Yang XY, Lai BY, Yang GY, and Liu JP
- Abstract
Background: Both cupping therapy and acupuncture have been used in China for a long time, and their target indications are pain-related conditions. There is no systematic review comparing the effectiveness of these two therapies., Objectives: To compare the beneficial effectiveness and safety between cupping therapy and acupuncture for pain-related conditions to provide evidence for clinical practice., Methods: Protocol of this review was registered in PROSPERO (CRD42016050986). We conducted literature search from six electronic databases until 31st March 2017. We included randomized trials comparing cupping therapy with acupuncture on pain-related conditions. Methodological quality of the included studies was evaluated by risk of bias tool. Mean difference, risk ratio, risk difference and their 95% confidence interval were used to report the estimate effect of the pooled results through meta-analysis or the results from each individual study. Trial sequential analysis (TSA) was applied to adjust random errors and calculate the sample size., Results: Twenty-three randomized trials with 2845 participants were included covering 12 pain-related conditions. All included studies were of poor methodological quality. Three meta-analyses were conducted, which showed similar clinical beneficial effects of cupping therapy and acupuncture for the rate of symptom improvement in cervical spondylosis (RR 1.13, 95% CI 1.01 to 1.26; n = 646), lateral femoral cutaneous neuritis (RR 1.10, 95% CI 1.00 to 1.22; n = 102) and scapulohumeral periarthritis (RR 1.31, 95% CI 1.15 to 1.51; n = 208). Results from other outcomes (such as visual analogue and numerical rating scale) in each study also showed no statistical significant difference between these two therapies for all included pain-related conditions. The results of TSA for cervical spondylosis demonstrated that the current available data have not reached a powerful conclusion. No serious adverse events related to cupping therapy or acupuncture was found in included studies., Conclusion: Cupping therapy and acupuncture are potentially safe, and they have similar effectiveness in relieving pain. However, further rigorous studies investigating relevant pain-related conditions are warranted to establish comparative effectiveness analysis between these two therapies. Cost-effectiveness studies should be considered in the future studies to establish evidence for decision-making in clinical practice.
- Published
- 2017
- Full Text
- View/download PDF
44. Characteristics of clinical studies of summer acupoint herbal patching: a bibliometric analysis.
- Author
-
Zhou F, Yang D, Lu JY, Li YF, Gao KY, Zhou YJ, Yang RX, Cheng J, Qi XX, Lai L, Lewith G, and Liu JP
- Subjects
- Case-Control Studies, Clinical Studies as Topic, Cross-Sectional Studies, Humans, Randomized Controlled Trials as Topic, Seasons, Acupuncture Points, Bibliometrics, Drugs, Chinese Herbal administration & dosage, Phytotherapy statistics & numerical data
- Abstract
Background: Summer acupoint herbal patching (SAHP) has been widely used in China for thousands of years. This bibliometric analysis aims to provide a comprehensive review of the characteristics of clinical studies on SAHP for any condition., Methods: We included clinical studies such as randomized clinical trials (RCTs), controlled clinical studies (CCTs), case series (CSs), case reports (CRs), and cross-sectional studies on SAHP for any condition. Six databases were searched from date of inception to March 2015. Bibliometric information and study details such as study type, characteristics of participants, details of the intervention and comparison, and outcome were extracted and analyzed., Results: A total of 937 clinical studies were identified and which were published between 1977 and 2015. This included 404 RCTs, 52 CCTs, 458 CSs, 19 CRs and 4 cross-sectional studies and involved 232,138 participants aged 2 to 90 years from two countries. Almost all studies were from China (936, 99.89%). The five conditions most commonly treated by SAHP were asthma (401, 42.80%), chronic bronchitis (146, 15.58%), allergic rhinitis (117, 12.49%), chronic obstructive pulmonary disease (73, 7.79%), and recurrent respiratory tract infection (42, 4.48%). Among 502 controlled studies, the majority compared SAHP alone with different controls (16 categories, 275 comparisons). The most commonly used controls were western medicine, placebo, traditional Chinese medicine, no treatment and non-pharmaceutical traditional Chinese therapies. Composite outcome measures were the most frequently reported outcome (512, 69.19%)., Conclusion: A substantial amount of research on SAHP has been published in China and which predominantly focuses on respiratory conditions. The findings from this study can be used to inform further research by highlighting areas of greatest impact for SAHP.
- Published
- 2015
- Full Text
- View/download PDF
45. Effect of Bizhongxiao decoction and its dismantled formulae on IL-1 and TNF levels in collagen-induced arthritis in rat synovial joints.
- Author
-
Guo YJ, Chen J, Xiong XG, Wu D, Zhu H, and Liang QH
- Subjects
- Animals, Arthritis, Experimental immunology, Female, Immunohistochemistry, Rats, Rats, Sprague-Dawley, Arthritis, Experimental drug therapy, Interleukin-1 analysis, Medicine, Chinese Traditional, Synovial Membrane chemistry, Tumor Necrosis Factor-alpha analysis
- Abstract
Background: Rheumatoid arthritis (RA), a chronic autoimmune disease, affects sufferers in many different ways. Treatment of this chronic condition is particularly challenging. Traditional Chinese Medicine (TCM) provides alternatives. Bizhongxiao decoction (BZX) is a TCM complex, which has been used clinically for many years to treat RA. The purpose of this study is to compare the effects of BZX decoction and its dismantled formulae on IL-1 and TNF-1 levels in rats with RA, and to elucidate its mechanism of action., Methods: Ninety healthy normal female SD rats were randomly divided into six groups: normal (control), model, BZX decoction, and the three dismantled formulae (I: heat-clearing and detoxication, II: dissipating dampness, and III: blood circulation promotion). Apart from the normal (control) group, the rats in each group were injected subcutaneously with bovine type II collagen and complete Freund adjuvant to establish a collagen-induced arthritis model, so that inhibition of foot swelling in the rats by BZX decoction and its dismantled formulae could be observed. Immunohistochemistry was used to assess the levels of the inflammatory cytokines IL-1 and TNF in synovial joints at various time points., Results: Twenty-one days after the model was established, the levels of TNF and IL-1 were significantly higher in the model group, BZX decoction group and dismantled formula groups I, II and III than in the normal controls (P < 0.05). The levels of these cytokines were significantly higher in the model group than the BZX decoction or the three dismantled formula groups (P <0.01). At longer times, the TNF and IL-1 levels in model group rose gradually; those in the BZX decoction and dismantled formula groups were gradually reduced. The cytokine levels in the BZX decoction group were lower than in the three dismantled formula groups and continued to decline., Conclusions: BZX decoction and the three dismantled formulae examined down-regulated the inflammatory factors IL-1 and TNF in collagen-induced arthritis rat models, but BZX exerted the strongest effect.
- Published
- 2012
- Full Text
- View/download PDF
46. Cholecystokinin receptor-1 mediates the inhibitory effects of exogenous cholecystokinin octapeptide on cellular morphine dependence.
- Author
-
Wen D, Ma CL, Zhang YJ, Meng YX, Ni ZY, Li SJ, and Cong B
- Subjects
- Analysis of Variance, Cell Line, Tumor, Colforsin pharmacology, Cyclic AMP metabolism, Dose-Response Relationship, Drug, Hormone Antagonists pharmacology, Humans, Naloxone pharmacology, Narcotic Antagonists pharmacology, Neuroblastoma, RNA, Messenger, Receptor, Cholecystokinin A genetics, Receptor, Cholecystokinin A metabolism, Receptor, Cholecystokinin B genetics, Receptor, Cholecystokinin B metabolism, Receptors, Cholecystokinin genetics, Receptors, Opioid, mu genetics, Receptors, Opioid, mu metabolism, Sincalide pharmacology, Morphine pharmacology, Narcotics pharmacology, Receptors, Cholecystokinin metabolism, Up-Regulation drug effects
- Abstract
Background: Cholecystokinin octapeptide (CCK-8), the most potent endogenous anti-opioid peptide, has been shown to regulate the processes of morphine dependence. In our previous study, we found that exogenous CCK-8 attenuated naloxone induced withdrawal symptoms. To investigate the precise effect of exogenous CCK-8 and the role of cholecystokinin (CCK) 1 and/or 2 receptors in morphine dependence, a SH-SY5Y cell model was employed, in which the μ-opioid receptor, CCK1/2 receptors, and endogenous CCK are co-expressed., Results: Forty-eight hours after treating SH-SY5Y cells with morphine (10 μM), naloxone (10 μM) induced a cAMP overshoot, indicating that cellular morphine dependence had been induced. The CCK receptor and endogenous CCK were up-regulated after chronic morphine exposure. The CCK2 receptor antagonist (LY-288,513) at 1-10 μM inhibited the naloxone-precipitated cAMP overshoot, but the CCK1 receptor antagonist (L-364,718) did not. Interestingly, CCK-8 (0.1-1 μM), a strong CCK receptor agonist, dose-dependently inhibited the naloxone-precipitated cAMP overshoot in SH-SY5Y cells when co-pretreated with morphine. The L-364,718 significantly blocked the inhibitory effect of exogenous CCK-8 on the cAMP overshoot at 1-10 μM, while the LY-288,513 did not. Therefore, the CCK2 receptor appears to be necessary for low concentrations of endogenous CCK to potentiate morphine dependence in SH-SY5Y cells. An additional inhibitory effect of CCK-8 at higher concentrations appears to involve the CCK1 receptor., Conclusions: This study reveals the difference between exogenous CCK-8 and endogenous CCK effects on the development of morphine dependence, and provides the first evidence for the participation of the CCK1 receptor in the inhibitory effects of exogenous CCK-8 on morphine dependence.
- Published
- 2012
- Full Text
- View/download PDF
47. Prevalence of canine leishmaniasis in Beichuan County, Sichuan, China and phylogenetic evidence for an undescribed Leishmania sp. in China based on 7SL RNA.
- Author
-
Sun K, Guan W, Zhang JG, Wang YJ, Tian Y, Liao L, Yang BB, Chen DL, and Chen JP
- Subjects
- Animals, China epidemiology, Dog Diseases epidemiology, Dogs, Endemic Diseases, Enzyme-Linked Immunosorbent Assay, Female, Humans, Insect Vectors parasitology, Leishmania genetics, Leishmaniasis, Visceral epidemiology, Leishmaniasis, Visceral parasitology, Male, Phylogeny, Polymerase Chain Reaction, Prevalence, Reagent Strips, Zoonoses epidemiology, Zoonoses parasitology, Dog Diseases parasitology, Leishmania isolation & purification, Leishmaniasis, Visceral veterinary, RNA, Small Cytoplasmic analysis, Signal Recognition Particle analysis
- Abstract
Background: Leishmaniasis is a vector-borne disease, which is still endemic in the west and northwest area of China. Canines are the major reservoirs of Leishmania, the etiological agent of human visceral leishmaniasis. Phlebotomus chinensis is the main transmission vector of zoonotic visceral leishmaniasis (ZVL)., Methods: In this study, rK39 dip-stick, ELISA and PCR methods were used to investigate the prevalence of canine leishmaniasis (CanL) in Beichuan County, Sichuan Province, China., Results: Among the 86 dogs which were included in the study, 13 dogs were positive using the dip-stick test (15.12%), while 8 dogs were positive using ELISA (9.30%) and 19 dogs were positive for PCR (22.03%). In total, 32 dogs were positive for one or more tests (37.21%). Interestingly, phylogenetic analysis based on the partial 7SL RNA fragment provided evidence that an undescribed Leishmania species, which is clearly a causative agent of CanL and human visceral leishmaniasis, does exist in China. This result is consistent with our previous study., Conclusions: Our work confirmed that canine leishmaniasis is still prevalent in Beichuan County. Further control is urgently needed, as canine leishmaniasis is of great public health importance. The phylogenetic analysis based on 7SL RNA segment provides evidence for the existence of an undescribed Leishmania sp. in China.
- Published
- 2012
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