7 results on '"Zhang, Wen-Jun"'
Search Results
2. Identifying risk factors for chronic kidney disease stage 3 in adults with acquired solitary kidney from unilateral nephrectomy: a retrospective cohort study
- Author
-
Zhang, Wen-Jun, Wang, Zi-Yi, Zhou, Wei-Xing, Yang, Ning-Qiang, Wang, Ya, Tang, Ya, Zhou, Xiao-Chun, Dao, Jie-Cao, Ma, Yan-Ru, He, Yan-Ping, Wang, Xiao-Ling, Wang, Wen-Ge, and Yang, Li
- Published
- 2020
- Full Text
- View/download PDF
3. Chemotherapy-induced niche perturbs hematopoietic reconstitution in B-cell acute lymphoblastic leukemia
- Author
-
Tang, Chao, Li, Ming-Hao, Chen, Ya-Li, Sun, Hui-Ying, Liu, Sheng-Li, Zheng, Wei-Wei, Zhang, Meng-Yi, Li, Hui, Fu, Wei, Zhang, Wen-Jun, Liang, Ai-Bin, Tang, Zhong-Hua, Hong, Deng-Li, Zhou, Bin-Bing S., and Duan, Cai-Wen
- Published
- 2018
- Full Text
- View/download PDF
4. The addition of a pH-sensitive gel improves microemulsion stability for the targeted removal of colonic ammonia.
- Author
-
Ai-Hong Wang, Zhi-Jun Duan, Ge Tian, Dan Lu, Wen-Jun Zhang, Gao-Hong He, Gui-Hua Fang, Wang, Ai-Hong, Duan, Zhi-Jun, Tian, Ge, Lu, Dan, Zhang, Wen-Jun, He, Gao-Hong, and Fang, Gui-Hua
- Subjects
COLON (Anatomy) ,AMMONIA ,GASTROINTESTINAL system ,HYDROCHLORIC acid ,MEDICAL research - Abstract
Background: We prepared an oral W/O microemulsion for the removal of colonic ammonia (ME-RCA). The effect of this microemulsion was influenced by the digestion process in the gastrointestinal tract. In this paper, we aim to show that stability was improved by using a microemulsion-based gel for the removal of colonic ammonia (MBG-RCA).Methods: MBG-RCA was prepared by adding sodium alginate to the ME-RCA. MBG-RCA and ME-RCA were passed through a simulated gastrointestinal environment, and the amount of colonic ammonia present was then determined by titration with a standard solution of hydrochloric acid. The pH of the gastrointestinal fluid was measured using a pH test paper and the size and form of the microemulsions were examined under the microscope. 18 healthy rats were randomly divided into three groups, fasted for 24 hours and allowed to drink normally. Three-way pipes were placed at the gastroduodenal junction in Group I, and at the terminal ileum in Group II. After the intragastric administration of ME-RCA, the stomach contents in Group I, the effluent from the terminal ileum in Group II and discharge from the anus in Group III were collected. The pH values of the gastrointestinal juice were measured by the pH test paper and those of the colon were determined by a universal indicator. These animal experiments were also used to test the effect of MBG-RCA.Results: MBG-RCA showed a better removal rate of artificial colonic ammonia than ME-RCA (P < 0.05). The decrease in pH value of the artificial small intestinal fluid due to ME-RCA did not occur when MBG-RCA was used. In the simulated gastrointestinal process, MBG-RCA maintained greater stability and released the emulsion (ME-RCA) in the colonic fluid. In the gastrointestinal tract of normal SD rats, ME-RCA decreased in size and lost its stable form after entering the small intestine, while MBG-RCA remained stable and intact emulsion-drops were observed from the anus. Neither substance had any effect on the pH of the stomach or colon of normal rats (partly because normal rats were fasted for 24 hours and allowed to drink normally, which resulted in a low level of ammonia production in the colon). Unlike ME-RCA, MBG-RCA did not reduce the pH of the small intestine.Conclusions: MBG-RCA was more stable in the gastrointestinal tract and more effective at removing colonic ammonia when a higher concentration of ammonia was present. This made it possible to achieve the targeted removal of colonic ammonia and is a promising method to prevent hepatic encephalopathy (HE) in future studies. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
5. A group LASSO-based method for robustly inferring gene regulatory networks from multiple time-course datasets.
- Author
-
Liu LZ, Wu FX, and Zhang WJ
- Subjects
- Cell Cycle genetics, DNA, Bacterial genetics, Escherichia coli genetics, Gene Expression Profiling, Saccharomyces cerevisiae cytology, Saccharomyces cerevisiae genetics, Time Factors, Algorithms, Computational Biology methods, Datasets as Topic, Gene Regulatory Networks
- Abstract
Background: As an abstract mapping of the gene regulations in the cell, gene regulatory network is important to both biological research study and practical applications. The reverse engineering of gene regulatory networks from microarray gene expression data is a challenging research problem in systems biology. With the development of biological technologies, multiple time-course gene expression datasets might be collected for a specific gene network under different circumstances. The inference of a gene regulatory network can be improved by integrating these multiple datasets. It is also known that gene expression data may be contaminated with large errors or outliers, which may affect the inference results., Results: A novel method, Huber group LASSO, is proposed to infer the same underlying network topology from multiple time-course gene expression datasets as well as to take the robustness to large error or outliers into account. To solve the optimization problem involved in the proposed method, an efficient algorithm which combines the ideas of auxiliary function minimization and block descent is developed. A stability selection method is adapted to our method to find a network topology consisting of edges with scores. The proposed method is applied to both simulation datasets and real experimental datasets. It shows that Huber group LASSO outperforms the group LASSO in terms of both areas under receiver operating characteristic curves and areas under the precision-recall curves., Conclusions: The convergence analysis of the algorithm theoretically shows that the sequence generated from the algorithm converges to the optimal solution of the problem. The simulation and real data examples demonstrate the effectiveness of the Huber group LASSO in integrating multiple time-course gene expression datasets and improving the resistance to large errors or outliers.
- Published
- 2014
- Full Text
- View/download PDF
6. An unsupervised machine learning method for assessing quality of tandem mass spectra.
- Author
-
Lin W, Wang J, Zhang WJ, and Wu FX
- Abstract
Background: In a single proteomic project, tandem mass spectrometers can produce hundreds of millions of tandem mass spectra. However, majority of tandem mass spectra are of poor quality, it wastes time to search them for peptides. Therefore, the quality assessment (before database search) is very useful in the pipeline of protein identification via tandem mass spectra, especially on the reduction of searching time and the decrease of false identifications. Most existing methods for quality assessment are supervised machine learning methods based on a number of features which describe the quality of tandem mass spectra. These methods need the training datasets with knowing the quality of all spectra, which are usually unavailable for the new datasets., Results: This study proposes an unsupervised machine learning method for quality assessment of tandem mass spectra without any training dataset. This proposed method estimates the conditional probabilities of spectra being high quality from the quality assessments based on individual features. The probabilities are estimated through a constraint optimization problem. An efficient algorithm is developed to solve the constraint optimization problem and is proved to be convergent. Experimental results on two datasets illustrate that if we search only tandem spectra with the high quality determined by the proposed method, we can save about 56 % and 62% of database searching time while losing only a small amount of high-quality spectra., Conclusions: Results indicate that the proposed method has a good performance for the quality assessment of tandem mass spectra and the way we estimate the conditional probabilities is effective.
- Published
- 2012
- Full Text
- View/download PDF
7. The addition of a pH-sensitive gel improves microemulsion stability for the targeted removal of colonic ammonia.
- Author
-
Wang AH, Duan ZJ, Tian G, Lu D, Zhang WJ, He GH, and Fang GH
- Subjects
- Animals, Drug Stability, Emulsions, Gastric Juice chemistry, Gastrointestinal Contents chemistry, Gels, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Hydrogen-Ion Concentration drug effects, Intestine, Small chemistry, Metabolic Clearance Rate, Rats, Rats, Sprague-Dawley, Stomach chemistry, Alginates chemistry, Ammonia chemistry, Ammonia metabolism, Colon chemistry, Pharmaceutical Preparations chemistry
- Abstract
Background: We prepared an oral W/O microemulsion for the removal of colonic ammonia (ME-RCA). The effect of this microemulsion was influenced by the digestion process in the gastrointestinal tract. In this paper, we aim to show that stability was improved by using a microemulsion-based gel for the removal of colonic ammonia (MBG-RCA)., Methods: MBG-RCA was prepared by adding sodium alginate to the ME-RCA. MBG-RCA and ME-RCA were passed through a simulated gastrointestinal environment, and the amount of colonic ammonia present was then determined by titration with a standard solution of hydrochloric acid. The pH of the gastrointestinal fluid was measured using a pH test paper and the size and form of the microemulsions were examined under the microscope. 18 healthy rats were randomly divided into three groups, fasted for 24 hours and allowed to drink normally. Three-way pipes were placed at the gastroduodenal junction in Group I, and at the terminal ileum in Group II. After the intragastric administration of ME-RCA, the stomach contents in Group I, the effluent from the terminal ileum in Group II and discharge from the anus in Group III were collected. The pH values of the gastrointestinal juice were measured by the pH test paper and those of the colon were determined by a universal indicator. These animal experiments were also used to test the effect of MBG-RCA., Results: MBG-RCA showed a better removal rate of artificial colonic ammonia than ME-RCA (P < 0.05). The decrease in pH value of the artificial small intestinal fluid due to ME-RCA did not occur when MBG-RCA was used. In the simulated gastrointestinal process, MBG-RCA maintained greater stability and released the emulsion (ME-RCA) in the colonic fluid. In the gastrointestinal tract of normal SD rats, ME-RCA decreased in size and lost its stable form after entering the small intestine, while MBG-RCA remained stable and intact emulsion-drops were observed from the anus. Neither substance had any effect on the pH of the stomach or colon of normal rats (partly because normal rats were fasted for 24 hours and allowed to drink normally, which resulted in a low level of ammonia production in the colon). Unlike ME-RCA, MBG-RCA did not reduce the pH of the small intestine., Conclusions: MBG-RCA was more stable in the gastrointestinal tract and more effective at removing colonic ammonia when a higher concentration of ammonia was present. This made it possible to achieve the targeted removal of colonic ammonia and is a promising method to prevent hepatic encephalopathy (HE) in future studies.
- Published
- 2011
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.