1. Comprehensive circRNA-microRNA-mRNA network analysis revealed the novel regulatory mechanism of Trichosporon asahii infection
- Author
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Xin Yang, Rongya Yang, Wan-Ting Luo, Zhi-Hong Zhu, Mingwang Zhang, Zhikuan Xia, and Junhong Ao
- Subjects
0301 basic medicine ,Leukocyte migration ,Trichosporon asahii ,03 medical and health sciences ,0302 clinical medicine ,Circular RNA ,Trichosporonosis ,microRNA ,Humans ,Medicine ,RNA, Messenger ,KEGG ,Genetics ,lcsh:R5-920 ,lcsh:Military Science ,Competing endogenous RNA ,business.industry ,Basidiomycota ,lcsh:U ,Research ,RNA ,RNA sequencing ,RNA, Circular ,General Medicine ,Non-coding RNA ,MicroRNAs ,030104 developmental biology ,030220 oncology & carcinogenesis ,lcsh:Medicine (General) ,business ,Signal Transduction - Abstract
Background Invasive Trichosporon asahii (T. asahii) infection frequently occurs with a high mortality in immunodeficient hosts, but the pathogenesis of T. asahii infection remains elusive. Circular RNAs (circRNAs) are a type of endogenous noncoding RNA that participate in various disease processes. However, the mechanism of circRNAs in T. asahii infection remains completely unknown. Methods RNA sequencing (RNA-seq) was performed to analyze the expression profiles of circRNAs, microRNAs (miRNAs), and mRNAs in THP-1 cells infected with T. asahii or uninfected samples. Some of the RNA-seq results were verified by RT-qPCR. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to analyze the differentially expressed mRNAs. A circRNA-miRNA-mRNA network was constructed and verified by dual-luciferase reporter assay and overexpression experiments. Results A total of 46 circRNAs, 412 mRNAs and 47 miRNAs were differentially expressed at 12 h after T. asahii infection. GO and KEGG analyses showed that the differentially expressed mRNAs were primarily linked to the leukocyte migration involved in the inflammatory response, the Toll-like receptor signaling pathway, and the TNF signaling pathway. A competing endogenous RNA (ceRNA) network was constructed with 5 differentially expressed circRNAs, 5 differentially expressed miRNAs and 42 differentially expressed mRNAs. Among them, hsa_circ_0065336 was found to indirectly regulate PTPN11 expression by sponging miR-505-3p. Conclusions These data revealed a comprehensive circRNA-associated ceRNA network during T. asahii infection, thus providing new insights into the pathogenesis of the T. asahii-host interactions.
- Published
- 2021