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Your search keyword '"mixed-phenotype acute leukemia"' showing total 2 results
Start Over Descriptor "mixed-phenotype acute leukemia" Publisher biomed central
2 results on '"mixed-phenotype acute leukemia"'

1. Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion.

Author

Nana Ping, Huiying Qiu, Qian Wang, Haiping Dai, Changgeng Ruan, Ehrentraut, Stefan, Drexler, Hans G., MacLeod, Roderick A. F., and Suning Chen

Subjects
*PHENOTYPES, *ACUTE leukemia, *CANCER cells, *CHIMERIC proteins, *SOMATIC mutation
Abstract

Herein, we describe the establishment and characterization of the first mixed-phenotype acute leukemia cell line (JIH-5). The JIH-5 cell line was established from leukemia cells with B lymphoid/myeloid phenotype from a female mixed-phenotype acute leukemia patient. JIH-5 cells exhibit an immunophenotype comprised of myeloid and B lymphoid antigens. Whole-exome sequencing revealed somatic mutations in nine genes in JIH-5 cells. Transcriptional sequencing of JIH-5 cells identified EP300-ZNF384 fusion transcript, which is a recurrent alteration in B cell acute lymphoblastic leukemia. Our results suggest that the JIH-5 cell line may serve as a tool for the study of mixed-phenotype acute leukemia or EP300-ZNF384. [ABSTRACT FROM AUTHOR]

Published
2015
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2. Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion

Author

Hans G. Drexler, Changgeng Ruan, Suning Chen, Haiping Dai, Huiying Qiu, Roderick A.F. MacLeod, Qian Wang, Stefan Ehrentraut, and Nana Ping

Subjects
Adoptive cell transfer, Cancer Research, Myeloid, Translocation, Genetic, Promyelocytic leukemia protein, Leukemia cell line, hemic and lymphatic diseases, Cell Line, Tumor, medicine, CD135, Humans, THP1 cell line, Molecular Biology, Letter to the Editor, Acute leukemia, ABL, Mixed-phenotype acute leukemia, biology, Hematology, Precursor Cell Lymphoblastic Leukemia-Lymphoma, medicine.disease, Leukemia, medicine.anatomical_structure, Phenotype, Oncology, Immunology, biology.protein, Cancer research, Next-generation sequencing, EP300-ZNF384, E1A-Associated p300 Protein, Transcription Factors
Abstract

Herein, we describe the establishment and characterization of the first mixed-phenotype acute leukemia cell line (JIH-5). The JIH-5 cell line was established from leukemia cells with B lymphoid/myeloid phenotype from a female mixed-phenotype acute leukemia patient. JIH-5 cells exhibit an immunophenotype comprised of myeloid and B lymphoid antigens. Whole-exome sequencing revealed somatic mutations in nine genes in JIH-5 cells. Transcriptional sequencing of JIH-5 cells identified EP300-ZNF384 fusion transcript, which is a recurrent alteration in B cell acute lymphoblastic leukemia. Our results suggest that the JIH-5 cell line may serve as a tool for the study of mixed-phenotype acute leukemia or EP300-ZNF384.

Published
2015

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