1. DNA-binding factor footprints and enhancer RNAs identify functional non-coding genetic variants.
- Author
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Biddie SC, Weykopf G, Hird EF, Friman ET, and Bickmore WA
- Subjects
- Humans, DNA Footprinting, DNA-Binding Proteins genetics, Genetic Variation, Enhancer Elements, Genetic, Enhancer RNAs, Genome-Wide Association Study, Polymorphism, Single Nucleotide, Quantitative Trait Loci
- Abstract
Background: Genome-wide association studies (GWAS) have revealed a multitude of candidate genetic variants affecting the risk of developing complex traits and diseases. However, the highlighted regions are typically in the non-coding genome, and uncovering the functional causative single nucleotide variants (SNVs) is challenging. Prioritization of variants is commonly based on genomic annotation with markers of active regulatory elements, but current approaches still poorly predict functional variants. To address this, we systematically analyze six markers of active regulatory elements for their ability to identify functional variants., Results: We benchmark against molecular quantitative trait loci (molQTL) from assays of regulatory element activity that identify allelic effects on DNA-binding factor occupancy, reporter assay expression, and chromatin accessibility. We identify the combination of DNase footprints and divergent enhancer RNA (eRNA) as markers for functional variants. This signature provides high precision, but with a trade-off of low recall, thus substantially reducing candidate variant sets to prioritize variants for functional validation. We present this as a framework called FINDER-Functional SNV IdeNtification using DNase footprints and eRNA., Conclusions: We demonstrate the utility to prioritize variants using leukocyte count trait and analyze variants in linkage disequilibrium with a lead variant to predict a functional variant in asthma. Our findings have implications for prioritizing variants from GWAS, in development of predictive scoring algorithms, and for functionally informed fine mapping approaches., (© 2024. The Author(s).) more...
- Published
- 2024
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