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Fungi were isolated using a rose bengal chloramphenicol agar as the culture medium. Congo red staining was used on sodium carboxymethyl cellulose medium to screen fungal strains that have potential to produce cellulolytic enzymes according to the cellulolytic index (CI). The ability of these isolates to break down holocellulose in three forest litter substrates (broad-leafed: Juglans mandshurica; coniferous: Larix gmelinii; broadleaf-conifer mixed: J. mandshurica and L. gmelinii) was tested over 80 days of incubation. The holocellulose content and the decomposition rule were studied. The strain with the most efficient degradation effect on natural cellulose in forest litter was selected. The growth of fungi was observed by scanning electron microscopy (SEM). The hydrolytic circles indicated the activity of cellulase produced by the fungi, and it implied that the fungi could degrade cellulose. The results showed that eight strains were able to degrade cellulose. The strain A2 (Peniophora incarnate) showed the highest CI, while A4 (Sarocladium strictum) was most capable of degrading holocellulose in various litter substrates. The SEM micrographs revealed that A4 had the ability to invade leaf tissue and degrade holocellulose in leaves. This study could be helpful for forest litter management, which provides a new way to cleanup forest litter using cellulose-degrading fungi.