1. Protective effect of cyanidin-3-O-glucoside on neonatal porcine islets
- Author
-
Mazzen Black, Bin Yang, Xian Li, Gina R. Rayat, Zhihao Xu, Ping Wu, Wenlong Huang, Kunsong Chen, Bao-You Xu, Bo Zhang, Eric Boivin, Chao Li, and Yulian Wu
- Subjects
0301 basic medicine ,medicine.medical_specialty ,MAP Kinase Signaling System ,NF-E2-Related Factor 2 ,Endocrinology, Diabetes and Metabolism ,Sus scrofa ,Transplantation, Heterologous ,Islets of Langerhans Transplantation ,Gene Expression ,medicine.disease_cause ,Antioxidants ,Anthocyanins ,Islets of Langerhans ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Glucosides ,Western blot ,Internal medicine ,medicine ,Extracellular ,Animals ,Phosphatidylinositol ,Protein kinase B ,Mitogen-Activated Protein Kinase 1 ,chemistry.chemical_classification ,geography ,Reactive oxygen species ,Mitogen-Activated Protein Kinase 3 ,geography.geographical_feature_category ,medicine.diagnostic_test ,Hydrogen Peroxide ,Islet ,Transplantation ,Oxidative Stress ,030104 developmental biology ,Animals, Newborn ,chemistry ,030220 oncology & carcinogenesis ,Phosphatidylinositol 3-Kinase ,Reactive Oxygen Species ,Heme Oxygenase-1 ,Oxidative stress ,Signal Transduction - Abstract
Oxidative stress is a major cause of islet injury and dysfunction during isolation and transplantation procedures. Cyanidin-3-O-glucoside (C3G), which is present in various fruits and vegetables especially in Chinese bayberry, shows a potent antioxidant property. In this study, we determined whether C3G could protect neonatal porcine islets (NPI) from reactive oxygen species (H2O2)-induced injury in vitro and promote the function of NPI in diabetic mice. We found that C3G had no deleterious effect on NPI and that C3G protected NPI from damage induced by H2O2. Significantly higher hemeoxygenase-1 (HO1) gene expression was detected in C3G-treated NPI compared to untreated islets before and after transplantation (P P
- Published
- 2017