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Your search keyword '"Galeati, G."' showing total 6 results
Start Over Author "Galeati, G." Publisher bioscientifica
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6. Gastric immunolocalization and plasma profiles of acyl-ghrelin in fasted and fasted-refed prepuberal gilts

Author

Giovanna Galeati, Uberto Pagotto, Renato Pasquali, Eraldo Seren, A. Parmeggiani, Cristina Cocco, R. De Iasio, Marcella Spinaci, Carlo Tamanini, Carla Brancia, Gian-Luca Ferri, Nadia Govoni, Govoni N., De Iasio R., Cocco C., Parmeggiani A., Galeati G., Pagotto U., Brancia C., Spinaci M., Tamanini C., Pasquali R., Ferri G-L., and Seren E.

Subjects
Blood Glucose, medicine.medical_specialty, Peptide Hormones, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Population, Radioimmunoassay, REFEEDING, Fatty Acids, Nonesterified, Peptide hormone, Biology, Eating, Endocrinology, Internal medicine, FASTING, medicine, Gastric mucosa, Animals, Insulin, GHRELIN, Sexual Maturation, SWINE, education, Chromatography, High Pressure Liquid, education.field_of_study, Stomach, digestive, oral, and skin physiology, Reproducibility of Results, Postprandial Period, Immunohistochemistry, METABOLIC PARAMETERS, medicine.anatomical_structure, Gastric Mucosa, Gastric pits, Female, Ghrelin, hormones, hormone substitutes, and hormone antagonists, Hormone
Abstract

Ghrelin is a peripheral circulating hormone, mainly released from the stomach, which can stimulate food intake. We studied fed, fasted and fasted-refed prepuberal gilts in order to outline possible changes in gastric mucosal ghrelin cells and in plasma ghrelin profiles in reponse to food deprivation. Acyl-ghrelin-immunoreactive cells were numerous in oxyntic glands, less abundant in cardiac glands and least frequent in pyloric glands, with the addition of a minor population of labelled cells in the gastric pit mucosa. When fed and fasted animals were compared (72-h fast versus fed; n=4 each), no clear-cut differences were revealed in labelled cell numbers, nor in their staining intensity. An RIA for plasma porcine acyl-ghrelin (n-octanoylated at Ser-3), not recognizing des-acyl-ghrelin, was validated. Plasma acyl-ghrelin progressively increased upon fasting (over 6, 12, 24 and 48 h); ghrelin levels significantly (P

Published
2005
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