1. Array-CGH identifies cyclin D1 and UBCH10 amplicons in anaplastic thyroid carcinoma
- Author
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Nimrod B Kiss, Amy Y. M. Au, Catharina Larsson, Åke Borg, Jia-Jing Lee, Michela Barbaro, Theodoros Foukakis, Leigh Delbridge, Johan Staaf, Göran Wallin, Bruce G. Robinson, Anders Höög, and Roderick J. Clifton-Bligh
- Subjects
Male ,Proto-Oncogene Proteins B-raf ,Cancer Research ,Endocrinology, Diabetes and Metabolism ,Chromosomes, Human, Pair 20 ,Gene Dosage ,Viral Oncogene ,Biology ,Pathogenesis ,Endocrinology ,Cyclin D1 ,CDKN2A ,medicine ,Humans ,Thyroid Neoplasms ,Anaplastic thyroid cancer ,neoplasms ,Aged ,Oligonucleotide Array Sequence Analysis ,Aged, 80 and over ,Comparative Genomic Hybridization ,medicine.diagnostic_test ,Gene Expression Profiling ,Carcinoma ,Gene Amplification ,Middle Aged ,medicine.disease ,Molecular biology ,Genes, bcl-1 ,Oncology ,Mutation ,Ubiquitin-Conjugating Enzymes ,Cancer research ,Female ,Sarcoma ,V600E ,Fluorescence in situ hybridization - Abstract
Anaplastic thyroid cancer (ATC) is a rare but highly aggressive disease with largely unexplained etiology and molecular pathogenesis. In this study, we analyzed genome-wide copy number changes, BRAF (V-raf sarcoma viral oncogene homolog B1) mutations, and p16 and cyclin D1 expressions in a panel of ATC primary tumors. Three ATCs harbored the common BRAF mutation V600E. Using array-comparative genomic hybridisation (array-CGH), several distinct recurrent copy number alterations were revealed including gains in 16p11.2, 20q11.2, and 20q13.12. Subsequent fluorescence in situ hybridization revealed recurrent locus gain of UBCH10 in 20q13.12 and Cyclin D1 (CCND1) in 11q13. The detection of a homozygous loss encompassing the CDKN2A locus in 9p21.3 motivated the examination of p16 protein expression, which was undetectable in 24/27 ATCs (89%). Based on the frequent gain in 11q13 (41%; n=11), the role of CCND1 was further investigated. Expression of cyclin D1 protein was observed at varying levels in 18/27 ATCs (67%). The effect of CCND1 on thyroid cell proliferation was assessed in vitro in ATC cells by means of siRNA and in thyroid cells after CCND1 transfection. In summary, the recurrent chromosomal copy number changes and molecular alterations identified in this study may provide an insight into the pathogenesis and development of ATC.
- Published
- 2008