1. Effects of dipyrone and acetylsalicylic acid on contractions of distal cauda epididymis duct, serum testosterone and sperm count in rats.
- Author
-
Martins ABM, Bezerra MS, da Costa Gomes LT, Trajano FMG, Dantas PB, de Sena MO, Gavioli EC, and da Silva Junior ED
- Subjects
- Animals, Aspirin, Dipyrone, Epididymis physiology, Genitalia drug effects, Male, Rats, Rats, Wistar, Sperm Count, Testosterone blood, Testosterone metabolism, Epididymis drug effects, Spermatozoa drug effects
- Abstract
The effects of dipyrone and acetylsalicylic acid (ASA) on male fertility are still not fully understood, mainly considering the epididymis as a putative target for their anti-fertility effects. Therefore, this study aimed to investigate the effects of dipyrone and ASA on the contractions of distal cauda epididymis duct, serum testosterone levels and sperm parameters in rats. Firstly, we checked the in vitro effects of dipyrone and ASA (10-1000 μM) on the contractions of distal cauda epididymis duct by pharmacological experiments. We also evaluated the effects of in vivo treatment with dipyrone and ASA 100 mg/kg (p.o.) for 15 days on epididymal duct contractions, serum testosterone levels and sperm parameters. In vitro dipyrone or ASA decreased the epididymal duct contractions induced by phenylephrine or carbachol. We observed that in vivo treatment with both drugs decreased the daily sperm production, serum testosterone levels and sperm count through epididymis without altering the epididymal duct contractions and sperm transit time through epididymis. In conclusion, in vitro dipyrone and ASA were able to diminish the contractions of epididymal duct, whilst in vivo administration decreased the sperm count throughout epididymis as a consequence of a low sperm production caused by reduced testosterone levels., (© 2021 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society).)
- Published
- 2021
- Full Text
- View/download PDF