1. Promotion of acute-phase skin wound healing by Pseudomonas aeruginosa C 4 -HSL.
- Author
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Kanno E, Kawakami K, Miyairi S, Tanno H, Suzuki A, Kamimatsuno R, Takagi N, Miyasaka T, Ishii K, Gotoh N, Maruyama R, and Tachi M
- Subjects
- 4-Butyrolactone pharmacology, Acute Disease, Analysis of Variance, Animals, Biopsy, Needle, Disease Models, Animal, Female, Homoserine analogs & derivatives, Homoserine pharmacology, Immunohistochemistry, Neutrophils metabolism, Random Allocation, Rats, Rats, Sprague-Dawley, Statistics, Nonparametric, Tumor Necrosis Factor-alpha metabolism, 4-Butyrolactone analogs & derivatives, Pseudomonas aeruginosa enzymology, Wound Healing physiology, Wounds and Injuries pathology, Wounds and Injuries therapy
- Abstract
A Pseudomonas aeruginosa quorum-sensing system, which produces N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C
12 -HSL) and N-butanoyl-l-homoserine lactone (C4 -HSL), regulates the virulence factors. In our previous study, 3-oxo-C12 -HSL, encoded by lasI gene, was shown to promote wound healing. However, the effect of C4 -HSL, encoded by rhlI gene, remains to be elucidated. We addressed the effect of C4 -HSL on wounds in P. aeruginosa infection. Wounds were created on the backs of Sprague-Dawley SD rats, and P. aeruginosa PAO1 (PAO1) or its rhlI deletion mutant (ΔrhlI) or lasI deletion mutant (ΔlasI) was inoculated onto the wound. Rats were injected intraperitoneally with anti-C4 -HSL antiserum or treated with C4 -HSL at the wound surface. PAO1 inoculation led to significant acceleration of wound healing, which was associated with neutrophil infiltration and TNF-α synthesis. These responses were reversed, except for TNF-α production, when ΔrhlI was inoculated instead of PAO1 or when rats were co-treated with PAO1 and anti-C4 -HSL antiserum. In contrast, the healing process and neutrophil infiltration, but not TNF-α synthesis, were accelerated when C4 -HSL was administered in the absence of PAO1. This acceleration was not affected by anti-TNF-α antibody. These results suggest that C4 -HSL may be involved in the acceleration of acute wound healing in P. aeruginosa infection by modifying the neutrophilic inflammation., (© 2015 Medicalhelplines.com Inc and John Wiley & Sons Ltd.)- Published
- 2016
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