1. Breast cyst fluids increase the proliferation of breast cell lines in correlation with their hormone and growth factor concentration.
- Author
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Enriori PJ, Vázquez SM, Chiauzzi V, Pérez C, Fischer CR, Gori JR, Etkin AE, Charreau E, Calandra RS, and Lüthy IA
- Subjects
- Adult, Body Fluids chemistry, Body Fluids metabolism, Cell Line, Tumor metabolism, Cell Proliferation, Epidermal Growth Factor analysis, Epithelial Cells pathology, Female, Humans, Hydrogen-Ion Concentration, Insulin-Like Growth Factor Binding Protein 3 analysis, Insulin-Like Growth Factor I analysis, Potassium analysis, Sodium analysis, Transforming Growth Factor beta analysis, Breast Cyst metabolism, Cell Line, Tumor pathology, Growth Hormone metabolism, Hormones metabolism
- Abstract
Objective and Design: Gross cystic disease (GCD) of the breast is reported to occur in 7% of women in the developed world and, although not premalignant, is thought to be associated with an increased risk of breast cancer. Hormone and growth factor concentration levels were measured in breast cyst fluid (BCF) to correlate them with their mitogenic activity in tumour (MCF-7) or nontransformed (MCF-10A) cells., Results: Oestradiol (E2), oestrone (E1), E2-sulfate (E2-S), E1-sulfate (E1-S) and epidermal growth factor (EGF) concentrations were, as expected, significantly higher in type I than in type II cysts, while transforming growth factor-beta 2 (TGF-beta2) showed higher levels in type II cysts. Fifty per cent of the BCF samples stimulated [3H]-thymidine incorporation into MCF-7 cells while 34.5% inhibited this parameter. In MCF-10A cells, most BCF samples were stimulatory (85%). E2, E1 and EGF concentrations in BCF samples correlated significantly and positively with cell proliferation in MCF-7 cells, whereas a significant negative correlation was found for TGF-beta2. In MCF-10A cells, only E2-S and E1-S exhibited significant positive correlation, whereas a significant negative correlation was found for TGF-beta2. Progesterone (Pg), E2 and EGF incubated under the same conditions had a stimulatory effect on [3H]-thymidine incorporation into MCF-7 cells, whereas TGF-beta2 inhibited this parameter. Pg, E2, E1 and EGF significantly stimulated this parameter in MCF-10A cells., Conclusions: The stimulatory action of BCF on cell proliferation in a model of human breast epithelial cells could partly explain the increased incidence of breast cancer in cyst-bearing women. more...
- Published
- 2006
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