1. Platelet freezing for serological purposes with and without a cryopreservative.
- Author
-
Helmerhorst FM, ten Berge ML, van der Plas-van Dalen CM, Engelfriet CP, and von dem Borne AE
- Subjects
- Autoantibodies, Dimethyl Sulfoxide, Fluorescent Antibody Technique, Freezing, Humans, Isoantibodies, Male, Platelet Aggregation, Blood Platelets immunology, Blood Preservation methods
- Abstract
We compared the reactivity of fresh platelets with platelets frozen in dimethylsulfoxide (DMSO) or without a cryopreservative by testing agglutinating and non-agglutinating platelet-specific alloantibodies in titration. Frozen platelets, irrespective of the method of preservation, showed no loss of antigenicity as compared with fresh platelets. The yield of platelets frozen without a cryopreservative proved to be higher (80%) than platelets frozen in the presence of DMSO (50%). Platelets sensitized with autoantibodies from patients with autoimmune thrombocytopenia could also be stored by simple freezing in platelet-rich plasma and used for (re)testing.
- Published
- 1984
- Full Text
- View/download PDF