1. Conserved lipoprotein H.8 of pathogenic Neisseria consists entirely of pentapeptide repeats.
- Author
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Woods JP, Spinola SM, Strobel SM, and Cannon JG
- Subjects
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Bacterial, Immunoblotting, Lipoproteins genetics, Molecular Sequence Data, Mutation, Bacterial Outer Membrane Proteins genetics, Genes, Genes, Bacterial, Neisseria gonorrhoeae genetics, Neisseria meningitidis genetics, Repetitive Sequences, Nucleic Acid
- Abstract
The pathogenic Neisseria, N. gonorrhoeae and N. meningitidis, possess an outer membrane protein (OMP), designated H.8, with a conserved monoclonal antibody (MAb)-binding epitope. We determined the DNA sequence of a gonococcal H.8 gene, and confirmed the relationship between the cloned gene and the H.8 OMP by constructing a gonococcal mutant lacking H.8. The predicted H.8 OMP is a lipoprotein 71 amino acids in length, composed of 13 repeats of a consensus sequence AAEAP with perfect 5-residue periodicity. The AAEAP units form a repeating epitope that comprises the entire predicted sequence of the protein. more...
- Published
- 1989
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