1. The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses.
- Author
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Téllez-Robledo B, Manzano C, Saez A, Navarro-Neila S, Silva-Navas J, de Lorenzo L, González-García MP, Toribio R, Hunt AG, Baigorri R, Casimiro I, Brady SM, Castellano MM, and Del Pozo JC
- Subjects
- 5' Untranslated Regions, Abscisic Acid metabolism, Alleles, Arabidopsis drug effects, Arabidopsis growth & development, Arabidopsis Proteins genetics, Cadmium toxicity, Cell Division genetics, Gene Expression Regulation, Plant genetics, Mutation, Phenotype, Plant Roots cytology, Plant Roots drug effects, Plant Roots genetics, Polyadenylation drug effects, Protein Biosynthesis genetics, RNA, Messenger genetics, RNA, Messenger metabolism, mRNA Cleavage and Polyadenylation Factors genetics, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Plant Roots metabolism, Polyadenylation genetics, Salt Stress genetics, mRNA Cleavage and Polyadenylation Factors metabolism
- Abstract
Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5' untranslated regions (5'-UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses., (© 2019 The Authors The Plant Journal © 2019 John Wiley & Sons Ltd.)
- Published
- 2019
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