4 results on '"Chen-Guang, Zhao"'
Search Results
2. LINGO-1 regulates Wnt5a signaling during neural stem and progenitor cell differentiation by modulating miR-15b-3p levels
- Author
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Chen-Guang Zhao, Jie Qin, Jia Li, Shan Jiang, Fen Ju, Wei Sun, Zhen Ren, Yu-Qiang Ji, Rui Wang, Xiao-Long Sun, Xiang Mou, and Hua Yuan
- Subjects
LINGO-1 ,Neural stem and progenitor cells ,Spinal cord injury ,Differentiation ,Wnt5a ,miR-15b-3p ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Manipulation of neural stem and progenitor cells (NSPCs) is critical for the successful treatment of spinal cord injury (SCI) by NSPC transplantation, since their differentiation into neurons and oligodendrocytes can be inhibited by factors present in inflamed myelin. In this study, we examined the effects of LINGO-1 on spinal cord-derived NSPC (sp-NSPC) differentiation, the underlying mechanisms of action, and the functional recovery of mice after transplantation of manipulated cells. Methods sp-NSPCs were harvested from female adult C57/BL6 mice after SCI induced with an NYU impactor. These cells were infected with lentiviral vectors containing LINGO-1 shRNA sequence or a scrambled control and transplanted into SCI mice. Tuj-1- and GFAP-positive cells were assessed by immunofluorescence staining. Wnt5a, p-JNK, JNK, and β-catenin expression was determined by Western blot and RT-qPCR. miRNAs were sequenced to detect changes in miRNA expression. Motor function was evaluated 0–35 days post-surgery by means of the Basso Mouse Scale (BMS) and by the rotarod performance test. Results We discovered that LINGO-1 shRNA increased neuronal differentiation of sp-NSPCs while decreasing astrocyte differentiation. These effects were accompanied by elevated Wnt5a protein expression, but unexpectedly, no changes in Wnt5a mRNA levels. miRNA-sequence analysis demonstrated that miR-15b-3p was a downstream mediator of LINGO-1 which suppressed Wnt5a expression. Transplantation of LINGO-1 shRNA-treated sp-NSPCs into SCI mice promoted neural differentiation, wound compaction, and motor function recovery. Conclusions LINGO-1 shRNA promotes neural differentiation of sp-NSPCs and Wnt5a expression, probably by downregulating miR-15b-3p. Transplantation of LINGO-1 shRNA-treated NSPCs promotes recovery of motor function after SCI, highlighting its potential as a target for SCI treatment.
- Published
- 2021
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3. LINGO-1 regulates Wnt5a signaling during neural stem and progenitor cell differentiation by modulating miR-15b-3p levels
- Author
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Xiao-Long Sun, Yu-Qiang Ji, Wei Sun, Fen Ju, Hua Yuan, Zhen Ren, Jie Qin, Shan Jiang, Rui Wang, Chen-Guang Zhao, Xiang Mou, and Jia Li
- Subjects
Medicine (General) ,Neural stem and progenitor cells ,Medicine (miscellaneous) ,Nerve Tissue Proteins ,Spinal cord injury ,QD415-436 ,Biology ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Biochemistry ,Wnt-5a Protein ,Rotarod performance test ,Small hairpin RNA ,Mice ,Myelin ,Astrocyte differentiation ,R5-920 ,Neural Stem Cells ,microRNA ,medicine ,Animals ,Progenitor cell ,Spinal Cord Injuries ,Research ,Membrane Proteins ,Cell Differentiation ,Cell Biology ,Wnt5a ,miR-15b-3p ,Cell biology ,Transplantation ,MicroRNAs ,medicine.anatomical_structure ,Differentiation ,Molecular Medicine ,Female ,Stem cell ,LINGO-1 - Abstract
Background Manipulation of neural stem and progenitor cells (NSPCs) is critical for the successful treatment of spinal cord injury (SCI) by NSPC transplantation, since their differentiation into neurons and oligodendrocytes can be inhibited by factors present in inflamed myelin. In this study, we examined the effects of LINGO-1 on spinal cord-derived NSPC (sp-NSPC) differentiation, the underlying mechanisms of action, and the functional recovery of mice after transplantation of manipulated cells. Methods sp-NSPCs were harvested from female adult C57/BL6 mice after SCI induced with an NYU impactor. These cells were infected with lentiviral vectors containing LINGO-1 shRNA sequence or a scrambled control and transplanted into SCI mice. Tuj-1- and GFAP-positive cells were assessed by immunofluorescence staining. Wnt5a, p-JNK, JNK, and β-catenin expression was determined by Western blot and RT-qPCR. miRNAs were sequenced to detect changes in miRNA expression. Motor function was evaluated 0–35 days post-surgery by means of the Basso Mouse Scale (BMS) and by the rotarod performance test. Results We discovered that LINGO-1 shRNA increased neuronal differentiation of sp-NSPCs while decreasing astrocyte differentiation. These effects were accompanied by elevated Wnt5a protein expression, but unexpectedly, no changes in Wnt5a mRNA levels. miRNA-sequence analysis demonstrated that miR-15b-3p was a downstream mediator of LINGO-1 which suppressed Wnt5a expression. Transplantation of LINGO-1 shRNA-treated sp-NSPCs into SCI mice promoted neural differentiation, wound compaction, and motor function recovery. Conclusions LINGO-1 shRNA promotes neural differentiation of sp-NSPCs and Wnt5a expression, probably by downregulating miR-15b-3p. Transplantation of LINGO-1 shRNA-treated NSPCs promotes recovery of motor function after SCI, highlighting its potential as a target for SCI treatment.
- Published
- 2021
4. Increased expression of collagens, transforming growth factor-β1, and -β3 in gluteal muscle contracture
- Author
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Haopeng Li, An-Jing Kang, Bin Lu, Chen-Guang Zhao, and Xijing He
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Contracture ,lcsh:Diseases of the musculoskeletal system ,Adolescent ,education ,Collagen Type I ,Transforming Growth Factor beta1 ,Extracellular matrix ,Young Adult ,Collagen Type III ,Transforming Growth Factor beta3 ,Rheumatology ,Downregulation and upregulation ,Transforming Growth Factor beta ,Fibrosis ,Research article ,Humans ,Medicine ,Genetic Predisposition to Disease ,Orthopedics and Sports Medicine ,RNA, Messenger ,Child ,Muscle, Skeletal ,Gluteal muscles ,biology ,business.industry ,Transforming growth factor beta ,medicine.disease ,Up-Regulation ,medicine.anatomical_structure ,biology.protein ,Buttocks ,Female ,Collagen ,medicine.symptom ,lcsh:RC925-935 ,business ,Biomarkers ,Transforming growth factor - Abstract
BackgroudGluteal muscle contracture (GMC) is a multi-factor human chronic fibrotic disease of the gluteal muscle. Fibrotic tissue is characterized by excessive accumulation of collagen in the muscle's extracellular matrix. Transforming growth factor (TGF)-β1 and -β2 are thought to play an important role in fibrogenesis, while TGF-β3 is believed to have an anti-fibrotic function. We hypothesize that the expression of collagen and TGF-βs would be up-regulated in GMC patients.MethodsThe expression of collagen type I, type III and TGF-βs were studied in 23 fibrotic samples and 23 normal/control samples in GMC patients using immunohistochemistry, reverse transcription and polymerase chain reaction (RT-PCR) and western bolt analysis.ResultsCompared to the unaffected adjacent muscle, increased expression of TGF-β1 and -β3 was associated with deposition of collagen type I and type III in the fibrotic muscle of the GMC patients at the mRNA level. Strong up-regulation of these proteins in fibrotic muscle was confirmed by immunohistochemical staining and western blot analysis. TGF-β2 was not up-regulated in relation to GMC.ConclusionThis study confirmed our hypothesis that collagen types I, III, TGF-β1 and TGF-β3 were up-regulated in biopsy specimens obtained from patients with GMC. Complex interaction of TGF-β1 with profibrotic function and TGF-β3 with antifibrotic function may increase synthesis of collagens and thereby significantly contribute to the process of gluteal muscle scarring in patients with GMC.
- Published
- 2010
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