1. Nasal gene expression differentiates COPD from controls and overlaps bronchial gene expression
- Author
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Ilse M. Boudewijn, Alen Faiz, Katrina Steiling, Erica van der Wiel, Eef D. Telenga, Susan J. M. Hoonhorst, Nick H. T. ten Hacken, Corry-Anke Brandsma, Huib A. M. Kerstjens, Wim Timens, Irene H. Heijink, Marnix R. Jonker, Harold G. de Bruin, J. Sebastiaan Vroegop, Henk R. Pasma, Wim G. Boersma, Pascal Wielders, Frank van den Elshout, Khaled Mansour, Avrum Spira, Marc E. Lenburg, Victor Guryev, Dirkje S. Postma, and Maarten van den Berge
- Subjects
COPD ,Nasal epithelium ,Bronchial epithelium ,Genome wide gene expression ,Microarray ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background Nasal gene expression profiling is a promising method to characterize COPD non-invasively. We aimed to identify a nasal gene expression profile to distinguish COPD patients from healthy controls. We investigated whether this COPD-associated gene expression profile in nasal epithelium is comparable with the profile observed in bronchial epithelium. Methods Genome wide gene expression analysis was performed on nasal epithelial brushes of 31 severe COPD patients and 22 controls, all current smokers, using Affymetrix Human Gene 1.0 ST Arrays. We repeated the gene expression analysis on bronchial epithelial brushes in 2 independent cohorts of mild-to-moderate COPD patients and controls. Results In nasal epithelium, 135 genes were significantly differentially expressed between severe COPD patients and controls, 21 being up- and 114 downregulated in COPD (false discovery rate
- Published
- 2017
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