1. Droplet digital PCR (ddPCR) for the detection and quantification of Ureaplasma spp.
- Author
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Yanfang Huang, Huifen Pan, Xiaoqin Xu, Panpan Lv, Xinxin Wang, and Zhen Zhao
- Subjects
Ureaplasma parvum ,Ureaplasma urealyticum ,qPCR ,Droplet digital PCR ,Absolute quantitation ,Cervicitis ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Ureaplasma spp. are associated with various infectious diseases in females, but there is still limited evidence regarding whether they are related to nonspecific cervicitis. The aim of this study was to develop and evaluate a digital droplet PCR (ddPCR) assay for the detection and quantification of Ureaplasma spp. in cervical swabs. Methods A total of 267 non-specific cervicitis (NSC) patients and 195 asymptomatic females were included in this study. We produced standard curves for Ureaplasma spp. to evaluate the analytical performance of the ddPCR assay. Then, we detected and quantified the bacterial load of Ureaplasma spp. in cervical swabs. Results The prevalences of U. parvum were 37.8% (101/267) and 29.7% (58/195), U. urealyticum were 9.0% (24/267) and 8.7% (17/195) in the NSC group and control group, respectively. In addition, the median copy number of U. parvum was 2.5 × 104 copies/ml (n = 101) in the NSC group and 9.2 × 103 copies/ml (n = 58) in the control group. The U. parvum load in the NSC group was significantly higher than that in the asymptomatic individuals (P
- Published
- 2021
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