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2 results on '"Kazuhiro Tanabe"'

1. Serum leucine-rich alpha-2-glycoprotein-1 with fucosylated triantennary N-glycan: a novel colorectal cancer marker

Author

Eiji Shinozaki, Kazuhiro Tanabe, Takashi Akiyoshi, Tomohiro Tsuchida, Yuko Miyazaki, Nozomi Kojima, Masahiro Igarashi, Masashi Ueno, Mitsukuni Suenaga, Nobuyuki Mizunuma, Kensei Yamaguchi, Konosuke Nakayama, Sadayo Iijima, and Toshiharu Yamaguchi

Subjects
Leucine-rich alpha-2-glycoprotein-1, Fucosylation, N-glycan, Colorectal cancer, Tumor marker, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background Carcinoembryonic antigen (CEA) and carbohydrate antigen (CA)19–9 are used in clinical practice as tumor markers to diagnose or monitor colorectal cancer (CRC) patients, However, their specificities and sensitivities are not ideal, and novel alternatives are needed. In this study, mass spectrometry was used to search for screening markers, focusing on glycan alterations of glycoproteins in the sera of CRC patients. Methods Glycopeptides were prepared from serum glycoproteins separated from blood samples of 80 CRC patients and 50 healthy volunteers, and their levels were measured by liquid chromatography time-of flight mass spectrometry (LC–TOF–MS). Results Leucine-rich alpha-2-glycoprotein-1 with fucosylated triantennary N-glycan (LRG–FTG) was identified as CRC marker after evaluating 30,000 candidate glycopeptide peaks. The average LRG–FTG level in CRC patients (1.25 ± 0.973 U/mL) was much higher than that in healthy volunteers (0.496 ± 0.433 U/mL, P

Published
2018
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2. Serum leucine-rich alpha-2-glycoprotein-1 with fucosylated triantennary N-glycan: a novel colorectal cancer marker

Author

Kensei Yamaguchi, Nozomi Kojima, Masashi Ueno, Kazuhiro Tanabe, Mitsukuni Suenaga, Yuko Miyazaki, Tomohiro Tsuchida, Nobuyuki Mizunuma, Takashi Akiyoshi, Eiji Shinozaki, Konosuke Nakayama, Masahiro Igarashi, Sadayo Iijima, and Toshiharu Yamaguchi

Subjects
Male, 0301 basic medicine, Cancer Research, Fucosylation, Colorectal cancer, Gastroenterology, 0302 clinical medicine, Carcinoembryonic antigen, Tandem Mass Spectrometry, Surgical oncology, Aged, 80 and over, chemistry.chemical_classification, biology, Middle Aged, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Glycopeptide, Oncology, 030220 oncology & carcinogenesis, N-glycan, Female, Colorectal Neoplasms, Research Article, Adult, Glycan, medicine.medical_specialty, CA-19-9 Antigen, Leucine-rich alpha-2-glycoprotein-1, lcsh:RC254-282, 03 medical and health sciences, Polysaccharides, Internal medicine, Biomarkers, Tumor, Genetics, medicine, Humans, Tumor marker, Aged, Glycoproteins, business.industry, medicine.disease, digestive system diseases, Carcinoembryonic Antigen, 030104 developmental biology, ROC Curve, chemistry, Case-Control Studies, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, biology.protein, Glycoprotein, business, Chromatography, Liquid
Abstract

Background Carcinoembryonic antigen (CEA) and carbohydrate antigen (CA)19–9 are used in clinical practice as tumor markers to diagnose or monitor colorectal cancer (CRC) patients, However, their specificities and sensitivities are not ideal, and novel alternatives are needed. In this study, mass spectrometry was used to search for screening markers, focusing on glycan alterations of glycoproteins in the sera of CRC patients. Methods Glycopeptides were prepared from serum glycoproteins separated from blood samples of 80 CRC patients and 50 healthy volunteers, and their levels were measured by liquid chromatography time-of flight mass spectrometry (LC–TOF–MS). Results Leucine-rich alpha-2-glycoprotein-1 with fucosylated triantennary N-glycan (LRG–FTG) was identified as CRC marker after evaluating 30,000 candidate glycopeptide peaks. The average LRG–FTG level in CRC patients (1.25 ± 0.973 U/mL) was much higher than that in healthy volunteers (0.496 ± 0.433 U/mL, P

Published
2018

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