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Your search keyword '"Akihito Endo"' showing total 4 results
Start Over Author "Akihito Endo" Publisher bmfh press
4 results on '"Akihito Endo"'

1. Microbial composition and metabolic profiles during machinecontrolled intra-factory fermentation of cocoa beans harvested in semitropical area of Japan.

Author

Hiroya NISHIMURA, Yuh SHIWA, Satoru TOMITA, and Akihito ENDO

Subjects
CACAO beans, FERMENTATION, HYDROPHILIC compounds, CHOCOLATE stores
Abstract

Cocoa bean fermentation is typically performed in a spontaneous manner on farms in tropical countries or areas and involves several microbial groups. Metabolism by microbes markedly affects the quality of cocoa beans fermented and the chocolate produced thereof. The present study characterized the microbiota and their metabolic profiles in temperature- and humidity-controlled intra-factory cocoa fermentation in a semitropical area of Japan. Although environmental factors were uniform, the microbiota of cocoa beans subjected to intrafactory fermentation was not stable between tests, particularly in terms of the cell count levels and species observed. Fermentation was sometimes delayed, and fermenting microbes were present at very low levels after 24 hr of fermentation. Due to the unstable microbiota, the profiles of water-soluble compounds differed between tests, indicating the unstable qualities of the fermented cocoa beans. These results suggest the necessity of starter cultures not only in on-farm fermentation but also in machine-controlled intra-factory cocoa fermentation. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Revealing the genomic differences between two subgroups in Lactobacillus gasseri

Author

Yasuhiro Tanizawa, Masanori Tohno, Ipputa Tada, Masanori Arita, and Akihito Endo

Subjects
0301 basic medicine, Comparative genomics, Genetics, 030106 microbiology, Immunology, Gastroenterology, Homoserine, Biology, Lactobacillus gasseri, biology.organism_classification, Applied Microbiology and Biotechnology, Microbiology, Genome, law.invention, 03 medical and health sciences, Probiotic, chemistry.chemical_compound, 030104 developmental biology, Plasmid, chemistry, law, Lactonase, biology.protein, Gene, Food Science
Abstract

Being an autochthonous species in humans, Lactobacillus gasseri is widely used as a probiotic for fermented products. We thoroughly compared the gene contents of 75 L. gasseri genomes and identified two intraspecific groups by the average nucleotide identity (ANI) threshold of 94%. Group I, with 48 strains, possessed 53 group-specific genes including the gassericin T cluster (9 genes) and N-acyl homoserine lactone lactonase. Group II, with 27 strains, including the type strain ATCC 33323, possessed group-specific genes with plasmid- or phage-related annotations. The genomic differences provide evidences for demarcating a new probiotic group within L. gasseri.

Published
2017
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3. Detection and analysis of Lactobacillus paracasei penicillin-binding proteins revealed the presence of cholate-sensitive penicillin-binding protein 3 and an elongated cell shape in a cholate-sensitive strain

Author

Glaezel Angelique Torres, Naoto Tanaka, Akihito Endo, Junichi Nakagawa, Masahiro Hattori, and Sanae Okada

Subjects
0301 basic medicine, Penicillin binding proteins, Lactobacillus paracasei, Immunology, medicine.disease_cause, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Cefalexin, polycyclic compounds, medicine, Escherichia coli, 030102 biochemistry & molecular biology, biology, Strain (chemistry), Gastroenterology, food and beverages, biology.organism_classification, Lactic acid, 030104 developmental biology, Biochemistry, chemistry, Biotinylation, Bacteria, Food Science, medicine.drug
Abstract

Penicillin-binding proteins (PBPs) are responsible for peptidoglycan synthesis. By using biotinylated ampicillin, we detected PBPs of Lactobacillus paracasei strains. Ten PBPs were identified, 7 of which had apparent molecular sizes similar to those of Escherichia coli. In the presence of cholate, strain NRIC 0625 showed an elongated shape, and its putative PBP3 showed cholate-sensitive penicillin-binding activity. Furthermore, this strain was highly sensitive to cefalexin, which is known to inhibit cell division by inactivating PBP3. These results suggest that the septum synthetase PBP3 of lactic acid bacteria can be one of the targets of intestinal bile acid.

Published
2017
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4. Lactobacillus apinorum belongs to the fructophilic lactic acid bacteria

Author

Leon M. T. Dicks, Junichi Nakagawa, Shintaro Maeno, and Akihito Endo

Subjects
0301 basic medicine, Genus Lactobacillus, biology, Immunology, Gastroenterology, food and beverages, biology.organism_classification, Applied Microbiology and Biotechnology, Microbiology, Lactic acid, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, 0302 clinical medicine, Lactobacillus apinorum, chemistry, 030211 gastroenterology & hepatology, Lactobacillus kunkeei, Gene, Bacteria, Food Science
Abstract

Potential fructophilic characteristics of Lactobacillus apinorum, originally isolated from the guts of honeybees (Apis mellifera), were studied in the present study. The species showed typical fructophilic growth characteristics, i.e., active growth on d-fructose, poor growth on d-glucose, and accelerated growth on d-glucose in the presence of electron acceptors. Biochemical characteristics strongly supported classification of the species into fructophilic lactic acid bacteria (FLAB). Furthermore, genetic analyses suggested that the species underwent extensive gene reduction, similar to that recorded for Lactobacillus kunkeei and other FLAB. These data clearly indicated that L. apinorum is the second fructophilic species within the genus Lactobacillus.

Published
2017
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