1. A luminance-based heart chip assay for assessing the efficacy of graft preservation solutions in heart transplantation in rats
- Author
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Eiji Kobayashi, Masashi Maeda, Yuki Iijima, Naoya Kasahara, Takeshi Kikuchi, Junshi Doi, and Takumi Teratani
- Subjects
Heart transplantation ,CHIP assay ,medicine.medical_specialty ,Pathology ,business.industry ,medicine.medical_treatment ,Urology ,Transplantation ,In vitro system ,medicine ,Preservation solutions ,Viability assay ,Cardiology and Cardiovascular Medicine ,business ,Saline ,Original Research ,Graft preservation - Abstract
Objective We developed a novel luciferase-based viability assay for assessing the viability of hearts preserved in different solutions. We examined whether this in vitro system could predict heart damage and survival after transplantation in rats. Design By our novel system, preserved heart viability evaluation and transplanted heart-graft functional research study. Setting University basic science laboratory. Interventions Isolated Luciferase-transgenic Lewis (LEW) rat cardiac-tissue-chips were plated on 96-well tissue-culture plates and incubated in preservation solutions at 4°C. Viability was measured as photon intensity by using a bio-imaging system. Heart-grafts preserved in University of Wisconsin (UW), extracellular-trehalose-Kyoto (ETK), Euro-Collins (EC), histidin-tryptophan-ketoglutarat solution (HTK), lactated Ringer's (LR) or normal saline solution were transplanted cervically by using a cuff-technique or into the abdomens of syngeneic wild-type LEW rats by using conventional microsurgical suture techniques. Main outcome measures Imaging an evaluation of preservation heart-graft and functional analysis. Results Cardiac-tissue-chips preserved with UW, HTK or ETK solution gave higher luminance than those preserved with EC, LR or normal saline (p
- Published
- 2013
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