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Your search keyword '"Bravata, V"' showing total 2 results
Start Over Author "Bravata, V" Publisher british institute of radiology
2 results on '"Bravata, V"'

1. Gene expression profiling of breast cancer cell lines treated with proton and electron radiations

Author

Bravata, V, Minafra, L, Cammarata, F, Pisciotta, P, Lamia, D, Marchese, V, Petringa, G, Manti, L, Cirrone, G, Gilardi, M, Cuttone, G, Forte, G, Russo, G, Bravata V., Minafra L., Cammarata F. P., Pisciotta P., Lamia D., Marchese V., Petringa G., Manti L., Cirrone G. A. P., Gilardi M. C., Cuttone G., Forte G. I., Russo G., Bravata, V, Minafra, L, Cammarata, F, Pisciotta, P, Lamia, D, Marchese, V, Petringa, G, Manti, L, Cirrone, G, Gilardi, M, Cuttone, G, Forte, G, Russo, G, Bravata V., Minafra L., Cammarata F. P., Pisciotta P., Lamia D., Marchese V., Petringa G., Manti L., Cirrone G. A. P., Gilardi M. C., Cuttone G., Forte G. I., and Russo G.

Abstract

objective: Technological advances in radiation therapy are evolving with the use of hadrons, such as protons, indicated for tumors where conventional radiotherapy does not give significant advantages or for tumors located in sensitive regions, which need the maximum of dose-saving of the surrounding healthy tissues. The genomic response to conventional and non-conventional linear energy transfer exposure is a poor investigated topic and became an issue of radiobiological interest. The aim of this work was to analyze and compare molecular responses in term of gene expression profiles, induced by electron and proton irradiation in breast cancer cell lines. Methods: We studied the gene expression profiling differences by cDNA microarray activated in response to electron and proton irradiation with different linear energy transfer values, among three breast cell lines (the tumorigenic MCF7 and MDA-MB-231 and the non-tumorigenic MCF10A), exposed to the same sublethal dose of 9 Gy. results: Gene expression profiling pathway analyses showed the activation of different signaling and molecular networks in a cell line and radiation type-dependent manner. MCF10A and MDA-MB-231 cell lines were found to induce factors and pathways involved in the immunological process control. conclusion: Here, we describe in a detailed way the gene expression profiling and pathways activated after electron and proton irradiation in breast cancer cells. Summarizing, although specific pathways are activated in a radiation type-dependent manner, each cell line activates overall similar molecular networks in response to both these two types of ionizing radiation.

Published
2018

2. Gene expression profiling of breast cancer cell lines treated with proton and electron radiations

Author

Francesco Paolo Cammarata, Maria Carla Gilardi, Giada Petringa, Debora Lamia, Valentina Bravatà, G.A.P. Cirrone, V. Marchese, Luigi Minafra, Giacomo Cuttone, Lorenzo Manti, Pietro Pisciotta, Giorgio Ivan Russo, Giusi Irma Forte, Bravata, V., Minafra, L., Cammarata, F. P., Pisciotta, P., Lamia, D., Marchese, V., Petringa, G., Manti, L., Cirrone, G. A. P., Gilardi, M. C., Cuttone, G., Forte, G. I., Russo, G., Bravata, V, Minafra, L, Cammarata, F, Pisciotta, P, Lamia, D, Marchese, V, Petringa, G, Manti, L, Cirrone, G, Gilardi, M, Cuttone, G, Forte, G, and Russo, G

Subjects
0301 basic medicine, Proton, Quantitative Biology::Tissues and Organs, medicine.medical_treatment, Nuclear Theory, Physics::Medical Physics, Gene Expression, Breast Neoplasms, Electrons, Electron, Adenocarcinoma, Radiation Tolerance, Cell Line, 03 medical and health sciences, 0302 clinical medicine, Conventional radiotherapy, Breast cancer cell line, Adenocarcinoma, Breast Neoplasms, Carcinoma, Ductal, Breast, Cell Line, Cell Line, Tumor, Electrons, Gene Expression, Humans, Linear Energy Transfer, Oligonucleotide Array Sequence Analysis, Precision Medicine, Radiation Tolerance, Gene Expression Profiling, Proton Therapy, Cell Line, Tumor, Proton Therapy, medicine, Humans, Linear Energy Transfer, Radiology, Nuclear Medicine and imaging, Precision Medicine, Nuclear Experiment, Oligonucleotide Array Sequence Analysis, FACTOR-KAPPA-B, TRANSCRIPTION FACTOR, INDUCED APOPTOSIS, ENERGY-TRANSFER, MONTE-CARLO, THERAPY, RADIOTHERAPY, SIMULATION, INHIBITION, PATHWAY, Full Paper, Chemistry, Gene Expression Profiling, Carcinoma, Ductal, Breast, General Medicine, Gene expression profiling, Radiation therapy, 030104 developmental biology, 030220 oncology & carcinogenesis, Cancer research
Abstract

OBJECTIVE: Technological advances in radiation therapy are evolving with the use of hadrons, such as protons, indicated for tumors where conventional radiotherapy does not give significant advantages or for tumors located in sensitive regions, which need the maximum of dose-saving of the surrounding healthy tissues. The genomic response to conventional and non conventional Linear Energy Transfer exposure is a poor investigated topic and became an issue of radiobiological interest. The aim of this work was to analyze and compare molecular responses in term of gene expression profiles, induced by electron and proton irradiation in breast cancer cell lines. METHODS: We studied the gene expression profiling differences by cDNA microarray activated in response to electron and proton irradiation with different Linear Energy Transfer values, among three breast cell lines (the tumorigenic MCF7 and MDA-MB-231 and the non tumorigenic MCF10A), exposed to the same sub-lethal dose of 9 Gy. RESULTS: Gene expression profiling pathway analyses showed the activation of different signaling and molecular networks in a cell line and radiation type-dependent manner. MCF10A and MDA-MB-231 cell lines were found to induce factors and pathways involved in the immunological process control. CONCLUSIONS: Here we describe in a detailed way the gene expression profiling and pathways activated after electron and proton irradiation in breast cancer cells. Summarizing, although specific pathways are activated in a radiation type-dependent manner, each cell line activates overall similar molecular networks in response to both these two types of ionizing radiation. Advances in knowledge: In the era of personalized medicine and breast cancer target-directed intervention, we trust that this study could drive radiation therapy towards personalized treatments, evaluating possible combined treatments, based on the molecular characterization.

Published
2018

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