1. Catalase expression impairs oxidative stress-mediated signalling in Trypanosoma cruzi.
- Author
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FREIRE, ANNA CLÁUDIA GUIMARÃES, ALVES, CERES LUCIANA, GOES, GRAZIELLE RIBEIRO, RESENDE, BRUNO CARVALHO, MORETTI, NILMAR SILVIO, NUNES, VINÍCIUS SANTANA, AGUIAR, PEDRO HENRIQUE NASCIMENTO, TAHARA, ERICH BIRELLI, FRANCO, GLÓRIA REGINA, MACEDO, ANDRÉA MARA, PENA, SÉRGIO DANILO JUNHO, GADELHA, FERNANDA RAMOS, GUARNERI, ALESSANDRA APARECIDA, SCHENKMAN, SERGIO, VIEIRA, LEDA QUERCIA, and MACHADO, CARLOS RENATO
- Subjects
TRYPANOSOMA cruzi ,OXIDATIVE stress ,HYDROGEN peroxide ,CELL lines ,CELL proliferation - Abstract
Trypanosoma cruzi is exposed to oxidative stresses during its life cycle, and amongst the strategies employed by this parasite to deal with these situations sits a peculiar trypanothione-dependent antioxidant system. Remarkably, T. cruzi’s antioxidant repertoire does not include catalase. In an attempt to shed light on what are the reasons by which this parasite lacks this enzyme, a T. cruzi cell line stably expressing catalase showed an increased resistance to hydrogen peroxide (H2O2) when compared with wild-type cells. Interestingly, preconditioning carried out with low concentrations of H2O2 led untransfected parasites to be as much resistant to this oxidant as cells expressing catalase, but did not induce the same level of increased resistance in the latter ones. Also, presence of catalase decreased trypanothione reductase and increased superoxide dismutase levels in T. cruzi, resulting in higher levels of residual H2O2 after challenge with this oxidant. Although expression of catalase contributed to elevated proliferation rates of T. cruzi in Rhodnius prolixus, it failed to induce a significant increase of parasite virulence in mice. Altogether, these results indicate that the absence of a gene encoding catalase in T. cruzi has played an important role in allowing this parasite to develop a shrill capacity to sense and overcome oxidative stress. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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