1. A MADS-box gene associated with protocorm-like body formation in Rosa canina alters floral organ development in Arabidopsis
- Author
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Jianxin Wu, Chunjing Li, Kun Liu, Mengzhu Zhao, Youwei Wang, Liangjun Zhao, Wei Wang, Yuling Zhu, Chengwei Li, and Kedong Xu
- Subjects
0106 biological sciences ,0301 basic medicine ,Somatic embryogenesis ,biology ,Callus formation ,Rosa canina ,fungi ,Plant Science ,Horticulture ,biology.organism_classification ,01 natural sciences ,Cell biology ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Arabidopsis ,Botany ,Ectopic expression ,Agronomy and Crop Science ,Abscisic acid ,Gene ,MADS-box ,010606 plant biology & botany - Abstract
We isolated a gene encoding a putative MADS-box protein designated RcAGL15 from a protocorm-like body (PLB), a somatic embryogenetic structure) of Rosa canina L. RcAGL15 encodes a predicted protein of 251 amino acid residues with a molecular weight of 28 kDa and a theoretical isoelectric point of 7.5. Reverse-transcription polymerase chain reaction shows that RcAGL15 is mainly expressed in PLB and regenerated PLB, but not in leaves of aseptic seedlings, incubated leaflets before callus formation, calli, rhizoids, or leaves of regenerated shoots. Transgenic, ectopic expression of RcAGL15 in Arabidopsis changes numbers of floral organs and reduces content of indole-3-acetic acid, but increases levels of abscisic acid. Thus, RcAGL15 appears to be a new MADS-box gene that plays regulatory roles in morphological development and the number of flower organs, as well as somatic embryogenesis.
- Published
- 2017
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