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There are 176 endemic plants spread in southern Colchis, of which 45 can be used for some medical treatments. The bioecology and detailed phytochemical content of some medicinal plant populations have not been studied so far. The research objectiveis to study the phytochemical content of endemic species of Helleborus caucasicus, Helleborus abchasicus and Ficaria popovii spread in southern Colchis. The research method for the phytochemical content included separation analysis, whichwas performed byusing UPLC-MS (Waters Acquity QDa detector). Three Steroidal glycosideswere isolated from the MeOH extract of the plants of Helleborus caucasicus and Helleborus abchasicus: Hellebrigenin-D-glucose, 20 – Hydroxyecdysone and Hydroxyecdysone – 3 glucoside. Two saponins (Hederagenin 3-O -α-L-arabino pyranoside, Hederagenin28-O-[α-L-rhamno-pyranosyl(1→4)-β-D-glucopyranosyl-(1→6)]β-D-lucopyranoside) and four flavonoids (kaempferol 3-O-β-ᴅ- (6ʺ-α-ʟ-rhamnopyranosyl)-glucopyranoside (nicotiflorin), apigenin 8-C-β-ᴅ-glucopyranoside (vitexin), luteolin 8-C-β-ᴅ-glucopyranoside (orientin), quercetin 3-O-rutinoside) were isolated from the tubers and flowers of Ficaria Popovii. Three Steroidal glycosides and Hydroxyecdysone -3 glucoside were isolated from the MeOH extract of Helleborus caucasicus.In addition, two saponins and four flavonoids were isolated from the tubers and leaves of FicariaPopovii.

Wild plants form the basis of biological resources both for Georgia and the whole world. A strategic task of any country is to preserve the biological diversity of plants. In the territory of Ajara, a large species diversity of plants grows, among which there are rare, endemic and relict plants. In particular, Cyclamen adzharicum. Modern systematics of wild plants in Georgia is based on classical methods of botany. In this regard, it is relevant to conduct genetic studies of species diversity and genetic polymorphism of species and populations using molecular genetic markers, in particular RAPD-PCR methods. The purpose of this study was to identify genetic polymorphism in Cyclamen L species using RAPD methods. As a result of the conducted research the 65 RAPD-markers in length from 150 to 1500 BP have been revealed. The number of the amplified fragments DNA varied depending on the primer from 6 (OPA-2) to 11 (OPB-4). The results of grouping Cyclamen adzharicum and C. coum samples allowed two clusters to be identified. In the first cluster were samples of three populations Cyclamen adzharicum and showed a low stubble in the intra-species variability. Cyclamen coum was attributed to the second cluster. The used primers gave the opportunity to identify polymorphism between the tested types of cyclamen.

We have researched Crataegus Monogina Jacq. microclonal propagation and in vitro mass regeneration processes. For this experiment we used the asleep buds isolated from maternal plants and in vitro cultivated apical and axillary buds as the effective explants. The high coefficient of micropropagation was provided with Gamborg Medium (B5) nutrient medium added alongside the hormones of cytokinin nature. The best result was achieved in the case of introduction of 2-Isopentenyladenine (2-iP) at concentration of 5-10 mcm and Benzilaminopurine (BAP) at concentration of 10-15 mcm. For stimulation of the mictropropagation process it is reasonable to add a small dose of the auxin hormone 1-Naphtylacetic acid (NAA) together with the cytokinins to the nutritive medium. It has been shown that the growth of micropropagation coefficient in pro rata the kinds and concentration of phytohormones. Regenerated microshoots had a good capacity of rooting. The most effective method for rooting purpose was the introduction of Indole- 3 –butyric acid (IBA) at concentration of 5mcm into 1 /2 B5 nutrient medium. The rooted regenerant plants successfully adopted in vivo.