1. Single-cell sequencing of immune cells after marathon and symptom-limited cardiopulmonary exercise.
- Author
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Yu Y, Zhang X, Chen Y, Li Y, Bian S, Yang Y, Song H, Zhang C, Dong Z, Li G, Xu L, Jia Z, Liu Y, He X, Yang M, Guo J, Zhou Z, and Zhang Y
- Abstract
Vigorous-intensity leisure-time physical activity, such as marathon, has become increasingly popular, but its effect on immune functions and health is poorly understood. Here, we performed scRNA-seq analysis of peripheral blood mononuclear cells (PBMCs) after a bout of symptom-limited cardiopulmonary exercise (CPX) test or marathon. Time-series single-cell analysis revealed the detailed series of landscapes of immune cells in response to short and long vigorous-intensity activities. Reduction of effective T cells was observed with the cell migration and motility pathways enriched in circulation following marathon. Baseline values of PBMCs abundance were reached around 1 h after CPX and 24 h following marathon, but longer time was required for expression recovery of cytotoxicity genes. The ratio of effector/naive T cells was found to change uniformly among the participants and could serve as a better indicator for exercise intensity than the CD4+/CD8+ T cell ratio. Moreover, we identified time-dependent monocyte state transitions after marathon., Competing Interests: Authors declare that they have no competing interests., (© 2023 The Author(s).)
- Published
- 2023
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