1. Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed
- Author
-
Nikolaus Rajewsky, Osnat Bartok, Panagiotis Papavasileiou, Reut Ashwal-Fluss, Mor Hanan, Christin Stottmeister, Petar Glažar, Agnieszka Rybak-Wolf, Marie Öhman, Sebastian Kadener, Natalia S. Pino, Margareta Herzog, Sebastian A. Giusti, Mikaela Behm, Damian Refojo, Marvin Jens, Andranik Ivanov, and Luisa Schreyer
- Subjects
Gene isoform ,Nervous system ,Genetics ,Gene knockdown ,Sequence analysis ,brain ,non-coding RNA ,RNA ,Cell Biology ,Biology ,Bioquímica y Biología Molecular ,Non-coding RNA ,biology.organism_classification ,Cell biology ,Ciencias Biológicas ,purl.org/becyt/ford/1 [https] ,medicine.anatomical_structure ,Circular RNA ,medicine ,Drosophila melanogaster ,purl.org/becyt/ford/1.6 [https] ,Molecular Biology ,CIENCIAS NATURALES Y EXACTAS ,circular RNAs - Abstract
Circular RNAs (circRNAs) are an endogenous class of animal RNAs. Despite their abundance, their function and expression in the nervous system are unknown. Therefore, we sequenced RNA from different brain regions, primary neurons, isolated synapses, as well as during neuronal differentiation. Using these and other available data, we discovered and analyzed thousands of neuronal human and mouse circRNAs. circRNAs were extraordinarily enriched in the mammalian brain, well conserved in sequence, often expressed as circRNAs in both human and mouse, and sometimes even detected in Drosophila brains. circRNAs were overall upregulated during neuronal differentiation, highly enriched in synapses, and often differentially expressed compared to their mRNA isoforms. circRNA expression correlated negatively with expression of the RNA-editing enzyme ADAR1. Knockdown of ADAR1 induced elevated circRNA expression. Together, we provide a circRNA brain expression atlas and evidence for important circRNA functions and values as biomarkers. Fil: Rybak Wolf, Agnieszka. Max-Delbrück Center for Molecular Medicine; Alemania Fil: Stottmeister, Christin. Max-Delbrück Center for Molecular Medicine; Alemania Fil: Glažar, Petar. Max-Delbrück Center for Molecular Medicine; Alemania Fil: Jens, Marvin. Max-Delbrück Center for Molecular Medicine; Alemania Fil: Pino, Natalia. Max Planck Institute of Psychiatry; Alemania Fil: Giusti, Sebastian. Max Planck Institute of Psychiatry; Alemania Fil: Hanan, Mor. The Hebrew University of Jerusalem; Israel Fil: Behm, Mikaela. Stockholms Universitet; Suecia Fil: Bartok, Osnat. The Hebrew University of Jerusalem; Israel Fil: Ashwal Fluss, Reut. The Hebrew University of Jerusalem; Israel Fil: Herzog, Margareta. Max-Delbrück Center for Molecular Medicine; Alemania Fil: Schreyer, Luisa. Max-Delbrück Center for Molecular Medicine; Alemania Fil: Papavasileiou, Panagiotis. Max-Delbrück Center for Molecular Medicine; Alemania Fil: Ivanov, Andranik. Max-Delbrück Center for Molecular Medicine; Alemania Fil: Öhman, Marie. Stockholms Universitet; Suecia Fil: Refojo, Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina. Max Planck Institute of Psychiatry; Alemania Fil: Kadener, Sebastian. The Hebrew University of Jerusalem; Israel Fil: Rajewsky, Nikolaus. Max-Delbrück Center for Molecular Medicine; Alemania
- Published
- 2014