1. Isolation and characterization of the CRIPTO autosomal gene and its X-linked related sequence.
- Author
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Dono R, Montuori N, Rocchi M, De Ponti-Zilli L, Ciccodicola A, and Persico MG
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Blotting, Southern, Cell Line, Chromosome Mapping, GPI-Linked Proteins, Humans, Intercellular Signaling Peptides and Proteins, Mice, Molecular Sequence Data, Multigene Family genetics, Mutation genetics, Tumor Cells, Cultured, Chromosomes, Human, Pair 3, Epidermal Growth Factor, Growth Substances genetics, Membrane Glycoproteins, Neoplasm Proteins genetics, X Chromosome
- Abstract
We have previously reported on the identification of a cDNA clone encoding a novel human growth factor, named "CRIPTO," that is abundantly expressed in undifferentiated human NTERA-2 clone D1 (NT2/D1) and mouse (F9) teratocarcinoma cells. We now report the organization and nucleotide sequence of two related genomic sequences. One (CR-1) corresponds to the structural gene encoding the human CRIPTO protein expressed in the undifferentiated human teratocarcinoma cells, and the other (CR-3) corresponds to a complete copy of the mRNA containing seven base substitutions in the coding region representing both silent and replacement substitutions. The 440 bp 5' to the CAP site of CR-1 are preserved in CR-3. CR-1 maps to chromosome 3, and CR-3 maps to Xq21-q22. Southern blot analysis reveals that multiple CRIPTO-related DNA sequences are present in the human as well as in the mouse genome.
- Published
- 1991