1. Neoplastic transformation of mast cells by Abelson-MuLV: abrogation of IL-3 dependence by a nonautocrine mechanism.
- Author
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Pierce JH, Di Fiore PP, Aaronson SA, Potter M, Pumphrey J, Scott A, and Ihle JN
- Subjects
- Animals, Cell Differentiation, Cell Division, Cell Line, Cells, Cultured, Clone Cells, Interleukin-3, Liver, Lymphokines biosynthesis, Mast Cells metabolism, Mast Cells physiology, Mast-Cell Sarcoma etiology, Mice, Mice, Inbred Strains, Receptors, Immunologic analysis, Receptors, Interleukin-3, Viral Proteins analysis, Abelson murine leukemia virus physiology, Cell Transformation, Neoplastic, Cell Transformation, Viral, Leukemia Virus, Murine physiology, Lymphokines pharmacology, Mast Cells microbiology
- Abstract
Normal mast cells can be propagated in culture when medium is supplemented with interleukin-3 (IL-3). We demonstrate that Abelson-MuLV (Ab-MuLV) infection of mast cells eliminates dependence on IL-3 for growth. By contrast, Harvey, BALB, and Moloney MSV, which also productively infect mast cells, are unable to relieve IL-3 dependence. Ab-MuLV-induced IL-3-independent lines express the v-abl-specific transforming protein and have phenotypic characteristics of mast cells. These cells also possess high cloning efficiencies in soft agarose and are tumorigenic in nude mice. In addition, Ab-MuLV induces transplantable mastocytomas in pristane-primed adult mice resistant to lymphoid transformation, defining a new hematopoietic target for malignant transformation by this virus. None of the Ab-MuLV-derived transformants express or secrete detectable levels of IL-3 nor is their growth inhibited by anti-IL-3 serum. These results argue that Ab-MuLV abrogation of the IL-3 requirement is not due to an autocrine mechanism.
- Published
- 1985
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