1. An in vitro assay to quantify satellite cell activation using isolated mouse myofibers.
- Author
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Canibano-Fraile R, Boertjes E, Bozhilova S, Pijnappel WWMP, and Schaaf GJ
- Subjects
- Animals, Mice, Cytological Techniques methods, Microscopy methods, Muscle Fibers, Skeletal cytology, Satellite Cells, Skeletal Muscle cytology, Satellite Cells, Skeletal Muscle metabolism, Satellite Cells, Skeletal Muscle physiology
- Abstract
Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation., Competing Interests: The authors declare no competing interests., (© 2021 The Author(s).)
- Published
- 2021
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