1. Distinct Polysaccharide Utilization Profiles of Human Intestinal Prevotella copri Isolates.
- Author
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Fehlner-Peach, Hannah, Magnabosco, Cara, Raghavan, Varsha, Scher, Jose U., Tett, Adrian, Cox, Laura M., Gottsegen, Claire, Watters, Aaron, Wiltshire-Gordon, John D., Segata, Nicola, Bonneau, Richard, and Littman, Dan R.
- Abstract
Gut-dwelling Prevotella copri (P. copri), the most prevalent Prevotella species in the human gut, have been associated with diet and disease. However, our understanding of their diversity and function remains rudimentary because studies have been limited to 16S and metagenomic surveys and experiments using a single type strain. Here, we describe the genomic diversity of 83 P. copri isolates from 11 human donors. We demonstrate that genomically distinct isolates, which can be categorized into different P. copri complex clades, utilize defined sets of polysaccharides. These differences are exemplified by variations in susC genes involved in polysaccharide transport as well as polysaccharide utilization loci (PULs) that were predicted in part from genomic and metagenomic data. Functional validation of these PULs showed that P. copri isolates utilize distinct sets of polysaccharides from dietary plant, but not animal, sources. These findings reveal both genomic and functional differences in polysaccharide utilization across human intestinal P. copri strains. • Human P. copri isolates have diverse genomes making up four distinct clades • Genome diversity is exemplified by differences in susC genes and predicted PULs • P. copri isolates utilize distinct sets of plant polysaccharides Fehlner-Peach et al. describe 83 P. copri isolates from the stool of 11 donors. Isolates have extensive genome variation and differences in polysaccharide utilization genes. In vitro growth experiments confirm that P. copri isolates utilize distinct sets of polysaccharides from dietary plant, but not animal, sources. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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