1. The Intracellular Cargo Receptor ERGIC-53 Is Required for the Production of Infectious Arenavirus, Coronavirus, and Filovirus Particles.
- Author
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Klaus, Joseph P., Eisenhauer, Philip, Russo, Joanne, Mason, Anne B., Do, Danh, King, Benjamin, Taatjes, Douglas, Cornillez-Ty, Cromwell, Boyson, Jonathan E., Thali, Markus, Zheng, Chunlei, Liao, Lujian, Yates, John R., Zhang, Bin, Ballif, Bryan A., and Botten, Jason W.
- Abstract
Summary: Arenaviruses and hantaviruses cause severe human disease. Little is known regarding host proteins required for their propagation. We identified human proteins that interact with the glycoproteins (GPs) of a prototypic arenavirus and hantavirus and show that the lectin endoplasmic reticulum (ER)-Golgi intermediate compartment 53 kDa protein (ERGIC-53), a cargo receptor required for glycoprotein trafficking within the early exocytic pathway, associates with arenavirus, hantavirus, coronavirus, orthomyxovirus, and filovirus GPs. ERGIC-53 binds to arenavirus GPs through a lectin-independent mechanism, traffics to arenavirus budding sites, and is incorporated into virions. ERGIC-53 is required for arenavirus, coronavirus, and filovirus propagation; in its absence, GP-containing virus particles form but are noninfectious, due in part to their inability to attach to host cells. Thus, we have identified a class of pathogen-derived ERGIC-53 ligands, a lectin-independent basis for their association with ERGIC-53, and a role for ERGIC-53 in the propagation of several highly pathogenic RNA virus families. [Copyright &y& Elsevier]
- Published
- 2013
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