1. Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis.
- Author
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Richon S, Zajac O, Perez Gonzalez C, and Matic Vignjevic D
- Subjects
- Mice, Animals, Disease Models, Animal, Immunohistochemistry, Tumor Microenvironment, Colonic Neoplasms genetics, Colonic Neoplasms pathology
- Abstract
The microenvironment plays an essential role in tumor development and metastatic progression. Here, we describe a simple and rapid protocol to generate tumors in mice using colon cancer cell lines or tumoroids in the correct microenvironment, colonic mucosa. We also detail steps for monitoring the growth of the primary tumor in real time using colonoscopy or in vivo imaging system, as well as monitoring metastasis development. Finally, we describe tissue collection and sample preparation for subsequent immunohistochemistry analysis., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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