1. Syntaxin 2 and Endobrevin Are Required for the Terminal Step of Cytokinesis in Mammalian Cells
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Noriko Kudo, Thomas Weimbs, Xin Li, Seng Hui Low, Masumi Miura, and Beatriz Quiñones
- Subjects
Time Factors ,Cell division ,Syntaxin 1 ,Nerve Tissue Proteins ,CHO Cells ,Ingression ,Biology ,Septin ,General Biochemistry, Genetics and Molecular Biology ,Cell Line ,R-SNARE Proteins ,Dogs ,Abscission ,Cricetinae ,Animals ,Syntaxin ,Molecular Biology ,Membrane Proteins ,Lipid bilayer fusion ,Cell Biology ,Rats ,Cell biology ,Midbody ,Antigens, Surface ,Cell Division ,Cytokinesis ,Developmental Biology - Abstract
The terminal step of cytokinesis in animal cells is the abscission of the midbody, a cytoplasmic bridge that connects the two prospective daughter cells. Here we show that two members of the SNARE membrane fusion machinery, syntaxin 2 and endobrevin/VAMP-8, specifically localize to the midbody during cytokinesis in mammalian cells. Inhibition of their function by overexpression of nonmembrane-anchored mutants causes failure of cytokinesis leading to the formation of binucleated cells. Time-lapse microscopy shows that only midbody abscission but not further upstream events, such as furrowing, are affected. These results indicate that successful completion of cytokinesis requires a SNARE-mediated membrane fusion event and that this requirement is distinct from exocytic events that may be involved in prior ingression of the plasma membrane.
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