1. Viral Metagenomic Analysis of Cerebrospinal Fluid from Patients with Acute Central Nervous System Infections of Unknown Origin, Vietnam
- Author
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Anh, Nguyen To, Nhu, Le Nguyen Truc, Hong, Nguyen Thi Thu, Phuc, Tran My, Tam, Pham Thi Thanh, Huong, Dang Thao, Anh, Tran Tuan, Deng, Xutao, Nghia, Ho Dang Trung, Nguyen, Tran Thua, Van Hung, Nguyen, Thuan, Nguyen Dac, Phuong, Pham Thi Hong, Chau, Nguyen Van Vinh, Baker, Stephen, Delwart, Eric, Thwaites, Guy, Van Tan, Le, VIZIONS Consortium1, Baker, Stephen [0000-0003-1308-5755], and Apollo - University of Cambridge Repository
- Subjects
Microbiology (medical) ,Viral metagenomics ,Echovirus ,Epidemiology ,viruses ,030231 tropical medicine ,lcsh:Medicine ,medicine.disease_cause ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,Central Nervous System Infections ,0302 clinical medicine ,Cerebrospinal fluid ,central nervous system infection ,Enterovirus Infections ,medicine ,Humans ,lcsh:RC109-216 ,030212 general & internal medicine ,Pathogen ,Cerebrospinal Fluid ,Hepatitis B virus ,Molluscum contagiosum virus ,biology ,enterovirus ,business.industry ,Research ,lcsh:R ,virus diseases ,biology.organism_classification ,Virology ,Infectious Diseases ,Vietnam ,Etiology ,Enterovirus ,next-generation sequencing ,meningitis/encephalitis ,Metagenomics ,viral metagenomics ,business ,Viral Metagenomic Analysis of Cerebrospinal Fluid from Patients with Acute Central Nervous System Infections of Unknown Origin, Vietnam - Abstract
Central nervous system (CNS) infection is a serious neurologic condition, although the etiology remains unknown in >50% of patients. We used metagenomic next-generation sequencing to detect viruses in 204 cerebrospinal fluid (CSF) samples from patients with acute CNS infection who were enrolled from Vietnam hospitals during 2012-2016. We detected 8 viral species in 107/204 (52.4%) of CSF samples. After virus-specific PCR confirmation, the detection rate was lowered to 30/204 (14.7%). Enteroviruses were the most common viruses detected (n = 23), followed by hepatitis B virus (3), HIV (2), molluscum contagiosum virus (1), and gemycircularvirus (1). Analysis of enterovirus sequences revealed the predominance of echovirus 30 (9). Phylogenetically, the echovirus 30 strains belonged to genogroup V and VIIb. Our results expanded knowledge about the clinical burden of enterovirus in Vietnam and underscore the challenges of identifying a plausible viral pathogen in CSF of patients with CNS infections.
- Published
- 2021
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