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The understanding of the increasing population density of copepods in culture tanks contributes to establish their stable cultures at high productions for aquaculture industry, which are preferred live feeds for fish larvae. In the present study, the semibenthic copepod Pseudodiaptomus nihonkaiensis were used as target species. This genus is often found from brackish water and its tolerance to a wide range of salinity and water quality has attracted attention as a potential commercial live feed for aquaculture. Population growth and egg production in the semi-benthic copepod P. nihonkaiensis are investigated. Copepods were cultured in tanks without mesh, with mesh lining tank walls, and with 2, 4 or 8 mesh partitions in each tank to identify the effect of substratum surface area on copepod population density. Linear regression revealed peaks in nauplius, copepodite, adult and total densities, and the cumulative number of eggs for all sampling events to correlate positively with substratum surface area (p<0.01). Greater substratum area within a culture tank may improve egg production by increasing habitat for ovigerous females. The simple installation of inexpensive artificial substrata in existing copepod culture tanks might improve the feasibility of using copepods as a live food in commercial-scale aquaculture [ABSTRACT FROM AUTHOR]