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Introduction. Streptococcus agalactiae are gram-positive, non-motile and encapsulated cocci. On blood agar, they produce an narrow zone of beta-haemolysis. This pathogen causes invasive bacterial diseases in newborns, including sepsis, meningitis, septicaemia, and pneumonia, when transmitted from infected mothers. Since S. agalactiae is a pathogen of primary concern for public health, this research has been conducted on it. The objective of the study is the isolation and molecular detection of virulence gene of S. agalactiae group B (GBS), and evaluation of the percentage of mother-to-child transmission of the pathogen. Materials and methods. A prospective cohort study was designed that included 300 pregnant women who were at more than 35 weeks of pregnancy. The gynaecologist collected 300 vaginal swabs from all participants in this study and followed up on all GBS-positive pregnant women after delivery to take swabs from their neonates. Traditional microbiological and molecular approaches were used to study isolated bacteria. Result. Sixty (20%) of three hundred pregnant women and 16 (26.6%) of their newborns were enrolled in this study. GBS was detected via culture methods and was confirmed by PCR with primers employed for the detection of atr gene (housekeeping gene). Positive isolates were 100% susceptible to antibiotics such as ceftriaxone, penicillin, and vancomycin, 93% were sensitive to chloramphenicol, 83% to erythromycin, and only 13% to tetracycline. Conclusion. Our data showed a high frequency of GBS infection in pregnant women and their newborns. A mandatory screening test and preventative medicine should be adopted to minimize the potentially fatal repercussions of this sickness.