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With the rapid development of electronic technology, many new promising ideas and technologies were introduced into thermal management, one of which is pulsating heat pipe (PHP), which is different from traditional heat transfer strategy. Hence, an investigation on pulsating heat pipe with multi turns will be conducted in order to examine nanoparticles effect on heat transport ability. The pulsating heat pipe consists of copper tube having inside radius of 1 mm, thickness of 0.5 mm and total length of 1605 mm. The investigation is done with acetone with a filling proportion of 60% for different % mass concentration of 1%, 2%, 3%, 4% and 5% of Al2O3 nanoparticles for differed heat contribution from 20 W to 60 W . From the results of different % mass concentration, the most reduced value of thermal resistance is 0.28 K/W at 1% mass concentration. Hence the PHP operates better with 1% mass concentration of Al2O3 nanoparticles.