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This paper studies the effect of gibberellic acid (GA3), herbal preparation (LAB1) and the pre-cooling treatment (Ph) on seed germination of Origanum heracleoticum L.and Origanum vulgare L. As a control variant, seed treatment with distilled water was used. The highest germination energy (EK), germination after 14 days (14D) and a total germination values (UK) were achieved with seeds of O. heracleoticum treated with 400ppm GA3 (50.3%, 57.3% and 64.8%, respectively) and the lowest values were observed with 3-day pre-cooling treatment (28.3%, 28.8% and 28.8%, respectively). The highest values for all seed quality parameters for O. vulgare were achieved by the treatment with the highest GA3 concentration, 1000 ppm (60.0%, 67.0% and 74.8%, respectively), while the lowest germination values were recorded in 4-day pre-cooling treatment (26.0%, 26.8% and 27.5%, respectively). Overall, higher germination was observed in seeds of O. vulgare. The best germination stimulitor for both species proved to be gibberellic acid (GA3) - a hormone of plant origin, followed by the herbal preparation (LAB1), then the control variant (DW). On the other hand, the most unfavourable to stimulate germination proved to be pre-cooling treatment (Ph), which had no impact on the increase of seeds germination. U radu je ispitivan uticaj giberelinske kiseline (GA3), pripravka od lekovitog bilja (LAB1) i prethodnog hlađenja (Ph) na klijavost semena vrsta Origanum heracleoticum L. i Origanum vulgare L. Kao kontrolna varijanta, korišćen je tretman semena destilovanom vodom (DW). Najveća energija klijanja (EK), klijavost nakon 14 dana (14D) i ukupna klijavost (UK) vrste O. heracleoticum ostvarene su kod semena tretiranog sa 400 ppm GA3 (50,3%, 57,3%, odnosno 64,8%), a najmanje vrednosti istih parametara su zabeležene pri tretmanu sa trodnevnim prethodnim hlađenjem (28,3%, 28,8%, odnosno 28,8%). Najveće vrednosti za sve ispitivane parametre kvaliteta semena vrste O. vulgare ostvarene su pri najvećoj GA3 koncentraciji od 1000 ppm (60,0%, 67,0%, odnosno 74,8%), dok su najmanje vrednosti kodove biljne vrste zabeležene pri tretmanu semena prethodnim četvorodnevnim hlađenjem (26,0%, 26,8% i 27,5%). Ukupno gledano, veću klijavost imalo je seme vrste O. vulgare.