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proyecto Malaspina-2010 (CSD2008-00077) del programa CONSOLIDER-INGENIO 2010, Ministerio de Ciencia e Innovación y proyecto EURO-BASIN (FP7-ENV-2010 264933) e Instituto Español de Oceanografia (IEO). C.M. recibió un contrato PFPI del IEO., The spatial variability of biomass and stable isotopes in plankton size fractions in the upper 200 m was studied in a high spatial resolution transect along 24°N from the Canary Islands to Florida to determine nitrogen and carbon sources. Vertical advection of waters predominated in lateral zones, while the central Atlantic (30–70°W) was characterized by strong stratification and oligotrophic surface waters. Plankton biomass was low in the central zone and high on both the eastern and the western sides, with most of the variability due to either large (>2000 µm) or small plankton (50% of organic nitrogen in the central zone, and even >30% in the eastern and the western zones. The impact of diazotrophy increased with the size of the organisms, supporting the wide participation of all trophic levels in the processing of recently fixed nitrogen. These results indicate that atmospheric sources of carbon and nitrogen prevail over deep water sources in the subtropical North Atlantic and that the zone influenced by diazotrophy is much larger than reported in previous studies., CONSOLIDER-INGENIO, 7FP, 1,7490